Multi-wavelength spectrophotometric determination of hydrogen peroxide in water by oxidative coloration of ABTS via Fenton reaction

Abstract

In this study, a sensitive and low-cost multi-wavelength spectrophotometric method for the determination of hydrogen peroxide (H₂O₂) in water was established. The method was based on the oxidative coloration of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) via Fenton reaction, which resulted in the formation of green radical (ABTS^•+) with absorbance at four different wavelengths (i.e., 415 nm, 650 nm, 732 nm, and 820 nm). Under the optimized conditions (C_ABTS = 2.0 mM, C_Fe²⁺ = 1.0 mM, pH = 2.60 ± 0.02, and reaction time (t) = 1 min), the absorbance of the generated ABTS^•+ at 415 nm, 650 nm, 732 nm, and 820 nm were well linear with H₂O₂ concentrations in the range of 0–40 μM (R² > 0.999) and the sensitivities of the proposed Fenton-ABTS method were calculated as 4.19 × 10⁴ M^–1 cm^–1,1.73 × 10⁴ M^–1 cm^–1, 2.18 × 10⁴ M^–1 cm^–1, and 1.96 × 10⁴ M^–1 cm^–1, respectively. Meanwhile, the detection limits of the Fenton-ABTS method at 415 nm, 650 nm, 732 nm, and 820 nm were respectively calculated to be 0.18 μM, 0.12 μM, 0.10 μM, and 0.11 μM. The absorbance of the generated ABTS^•+ in ultrapure water, underground water, and reservoir water was quite stable within 30 min. Moreover, the proposed Fenton-ABTS method could be used for monitoring the variations of H₂O₂ concentration during the oxidative decolorization of RhB in alkali-activated H₂O₂ system.