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Natural and non-toxic products from Fabaceae Brazilian plants as a replacement for traditional antifouling biocides: an inhibition potential against initial biofouling

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Abstract

In this study, we screened for the antifouling activity of 15 species plant extracts from Brazilian the Brazilian Caatinga Fabaceae against the initial colonization of natural marine bacterial biofilm. We also investigated the potential toxicity of extracts against planktonic and benthic non-target organisms. Aqueous extracts of plants collected in the Caatinga biome (PE, Brazil) were prepared and tested at different concentration levels (0, 0.5, 1, 2, 4, and 8 mg mL−1). Natural marine bacterial consortium was inoculated in multi-well plates and incubated with the different treatments for 48 h. The biofilm and planktonic bacterial density and biomass inhibition were evaluated along with biofilm biomass eradication. The extracts that showed the highest bacterial biofilm inhibition were evaluated for toxicity against microalgae and crustaceans. The biofilm and planktonic bacterial inhibition potential were evaluated through flow cytometry and spectrophotometry. The selected treatments were evaluated for their toxicity using the microalgae Chaetoceros calcitrans, the copepod Nitokra sp., and the brine shrimp Artemia salina as bioindicators. Our work demonstrates the biotechnological potential of Fabaceae plant compounds as a safe antifouling alternative. Anadenanthera colubrina var. cebil fruits and Apuleia leiocarpa leaf extracts showed antibiofilm activity (≥ 80%), while Myroxylon peruiferum and Dioclea grandiflora leaf extracts showed antibiotic activity. These extracts were safe to planktonic and benthic non-target organisms. The results of this study point to potential substitutes to highly toxic antifouling paints and shed light on the prospect of a yet to be explored biome for more sustainable alternatives in biofouling research.

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Acknowledgments

The authors acknowledge the support of Universidade Federal of Rio Grande (FURG), Universidade Federal do Rio Grande do Sul (UFRGS), Universidade Federal de Pernambuco (UFPE), Centro de Microscopia Eletrônica do Sul (CEME-SUL), and PNPD-CAPES scholarship.

Funding

This work was supported by the PRONEM FAPERGS/CNPq (16/2551-000244-4).

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Authors and Affiliations

  1. Laboratório de Microcontaminantes Orgânicos e Ecotoxicologia Aquática - Instituto de Oceanografia da Universidade Federal do Rio Grande (FURG), Caixa Postal, 474, Rio Grande, RS, CEP: 96203-900, Brazil

    Vanessa Ochi Agostini & Grasiela Lopes Leães Pinho

  2. Programa de Pós-graduação em Oceanologia (PPGO), Programa Nacional de Pós-Doutorado da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (PNPD-CAPES), Rio Grande, RS, Brazil

    Vanessa Ochi Agostini

  3. Laboratório de Biofilmes e Diversidade Microbiana - Faculdade de Farmácia e Centro de Biotecnologia da Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga, 2752, Bairro Azenha, Porto Alegre, RS, 90610-000, Brazil

    Alexandre José Macedo

  4. Laboratório de Zooplâncton - Instituto de Oceanografia da Universidade Federal do Rio Grande (FURG), Av. Itália, Km 8, Caixa Postal, 474, Rio Grande, RS, 96203-900, Brazil

    Erik Muxagata

  5. Laboratório de Produtos Naturais - Departamento de Bioquímica da Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE, 50670-901, Brazil

    Márcia Vanusa da Silva

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  1. Vanessa Ochi Agostini
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  2. Alexandre José Macedo
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  3. Erik Muxagata
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  4. Márcia Vanusa da Silva
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  5. Grasiela Lopes Leães Pinho
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Correspondence to Vanessa Ochi Agostini.

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The present research is in accordance with Brazilian legislation for the use of genetic patrimony and tradition-associated knowledge, under the record numbers: SisGen A08E18B and SisGen A50301E.

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The authors declare that they have no competing interests.

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Highlights

Traditional antifouling compounds are toxic and unsafe. Thus, new alternatives are needed. Caatinga plants from the Fabaceae family could be an effective friendly antifouling alternative. Fabaceae extracts showed marine antibiofilm activity (≥ 80%) and were safe to planktonic and benthic non-target organisms.

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Agostini, V.O., Macedo, A.J., Muxagata, E. et al. Natural and non-toxic products from Fabaceae Brazilian plants as a replacement for traditional antifouling biocides: an inhibition potential against initial biofouling. Environ Sci Pollut Res 26, 27112–27127 (2019). https://doi.org/10.1007/s11356-019-05744-4

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  • DOI: https://doi.org/10.1007/s11356-019-05744-4

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