Abstract
Brazilian population is one of the largest consumers of pesticides in the world, especially the Central Brazil population. Thus, the aim of this study was to evaluate the frequency of genotypes, alleles, haplotypes, and the linkage disequilibrium (DL) of the OGG1 gene in rural workers from Central Brazil, comparing with the populations of the 1000 genome. Three hundred thirty healthy individuals not related and randomly selected were included in this study. We obtained genomic DNA from peripheral blood lymphocytes. The 748-bp OGG1 gene was amplified by PCR and sequenced. Of the 330 individuals, 215 (65%) were males and 115 (35%) were females. There were no differences in the distribution of the rs1052133 and rs293795 with age and sexes. Haplotypes containing only conserved T/C alleles were the most common in our population. The frequency of the mutant alleles of rs1052133 and rs293795, in our population, was 20% and 30%, respectively, and it is noteworthy, worldwide, that mutant alleles are commonly associated to an increased risk for the development of cancer, specially due to direct or indirect contact to pesticides, as occurs in rural workers of Central Brazil population.
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Acknowledgments
AAA, HFN, DMS, and TNS thank CAPES and CNPq for their fellowships. The authors also thank FAPEG DOCFIX Edital 04/2014 (Process: 201510267000196). We finally thank the individuals that voluntarily participated of this study.
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All participants provided their informed consent and the study was approved by the Human Research Ethics Committee of the Universidade Federal de Goiás (Protocol 165.735). Demographic and clinical data were collected for a statistical survey.
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S1.
Electropherogram of three distinct aligned samples. The red arrow indicates rs 1052133. (A) Homozygous genotype of the G allele (B) Heterozygote C/G. (C) Homozygous genotype of ancestral C allele. (JPG 68 kb)
S2.
Electropherogram of three distinct aligned samples. The red arrow indicates rs 293795. (A) Homozygous genotype of the A allele. (B) Heterozygote A/G. (C) Homozygous genotype of the altered G allele. (JPG 56 kb)
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de Melo e Silva, D., Alves, A.A., Nunes, H.F. et al. Evaluating the OGG1 rs1052133 and rs293795 polymorphisms in a sample of rural workers from Central Brazil population: a comparative approach with the 1000 Genomes Project. Environ Sci Pollut Res 25, 25612–25617 (2018). https://doi.org/10.1007/s11356-018-2766-8
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DOI: https://doi.org/10.1007/s11356-018-2766-8