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Do GSTT1 and GSTM1 polymorphisms influence intoxication events in individuals occupationally exposed to pesticides?

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Abstract

This study evaluated the variability of GSTM1 and GSTT1 polymorphisms in individuals occupationally exposed to pesticides in ten Goias municipalities that present intense agricultural activity. We evaluated blood samples of 235 individuals, which 120 were rural workers occupationally exposed to pesticides and 115 formed the control group, analyzing GST polymorphisms by quantitative polymerase chain reaction (qPCR).The exposed group consisted of 111 men and nine women only getting an average of 39 ± 9 years. These workers were from ten rural municipalities situated at Goias state. It was found that 18 % of the exposed individuals had the GSTT1 null genotype and 49 % had the GSTM1 null genotype, and 10 % had both null genotypes. Data as intoxication (42 %), use of Personal Protection Equipment (PPE; 52 %) and if the worker prepared the pesticide (7 %), or if just applied the pesticide (22 %) or if the worker prepared and applied (71 %) have all been correlated with genetic polymorphisms. There were no statistically significant differences between the GSTM1 and GSTT1 polymorphisms between control and exposed groups. Finally, we could not associate a null GSTT1 or null GSTM1 polymorphisms or both to intoxication events caused by pesticides, but instead we presented the importance to use PPE to prevent such harm, once we found a statistically significant association between the use of PPE and events of intoxication (p ≤ 0.001).

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Acknowledgements

The authors thank the voluntary participation of the farmers and Dr. Sean Quail for proofreading this manuscript. This study was supported by several grants, as CNPq (Edital Universal 2010, Processo 478130/2010-4) and FAPEG (Pesquisas para Fortalecimento de Políticas Públicas em Goiás, Edital 03/2010). L.M.B, EOAC and M.P. are grateful for their government scholarship (CAPES).

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Authors and Affiliations

  1. Departamento de Biologia, Núcleo de Pesquisas Replicon, Rua 235, n. 40, Goiânia, Brazil

    Fernanda Ribeiro Godoy, Aldaires Vieira de Melo, Alex Silva Cruz, Lysa Bernardes Minasi, Cristiano Luiz Ribeiro & Aparecido Divino da Cruz

  2. Departamento de Biologia, Pontifícia Universidade Católica de Goiás, Bloco L, Área IV, Setor Universitário, Goiânia, 74605-050, GO, Brazil

    Fernanda Ribeiro Godoy, Aldaires Vieira de Melo, Alex Silva Cruz, Lysa Bernardes Minasi, Cristiano Luiz Ribeiro & Aparecido Divino da Cruz

  3. Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN quadra 916, Módulo B, Av. w5 norte, Asa Norte, Brasília, 70790-160, DF, Brazil

    Emília Oliveira Alves Costa

  4. Departamento de Biologia Geral, Programa de Pós-Graduação em Genética e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Campus Samambaia, Goiânia, 74001-970, Goiás, Brazil

    Angela Adamski da Silva Reis, Mariana Pedrosa Batista, Macks Wendhel Gonçalves, Caroline Oliveira de Araújo Melo & Daniela de Melo e Silva

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  1. Fernanda Ribeiro Godoy
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  2. Emília Oliveira Alves Costa
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  3. Angela Adamski da Silva Reis
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  4. Mariana Pedrosa Batista
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  5. Aldaires Vieira de Melo
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  6. Macks Wendhel Gonçalves
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  7. Alex Silva Cruz
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  8. Caroline Oliveira de Araújo Melo
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  9. Lysa Bernardes Minasi
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  10. Cristiano Luiz Ribeiro
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  11. Aparecido Divino da Cruz
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  12. Daniela de Melo e Silva
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Corresponding author

Correspondence to Daniela de Melo e Silva.

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Responsible editor: Philippe Garrigues

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Godoy, F.R., Costa, E.O.A., da Silva Reis, A.A. et al. Do GSTT1 and GSTM1 polymorphisms influence intoxication events in individuals occupationally exposed to pesticides?. Environ Sci Pollut Res 21, 3706–3712 (2014). https://doi.org/10.1007/s11356-013-2349-7

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  • DOI: https://doi.org/10.1007/s11356-013-2349-7

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