Environmental Science and Pollution Research

, Volume 24, Issue 30, pp 23815–23824 | Cite as

Effects of modified biochar on rhizosphere microecology of rice (Oryza sativa L.) grown in As-contaminated soil

  • Shusi Liu
  • Yixin Lu
  • Chen Yang
  • Chuanping Liu
  • Lin Ma
  • Zhi Dang
Research Article


Biochar was carbon-rich and generated by high-temperature pyrolysis of biomass under oxygen-limited conditions. Due to the limitations of surface functional groups and the weakness of surface activity in the field of environmental remediation, the raw biochar frequently was chemically modified to improve its properties with a new performance. In this study, a kind of high-efficiency and low-cost amino biochar modified by nano zero-valent iron (ABC/NZVI) was synthesized and applied to paddy soil contaminated with arsenic (As). Dynamic changes of soil properties, arsenic speciations and rhizosphere microbial communities have been investigated over the whole growth period of rice plants. Pot experiments revealed that the ABC/NZVI could decrease the arsenic concentration in rice straw by 47.9% and increase the content of nitrogen in rice straw by 47.2%. Proportion of Geobacter in soil with ABC/NZVI treatment increased by 175% in tillering period; while Nitrososphaera decreased by 61 and 20% in tillering and maturity, respectively, compared to that of control. ABC/NZVI promotes arsenic immobilization in rhizosphere soil and precipitation on root surface and reduces arsenic accumulation in rice. At the same time, ABC/NZVI would inhibit Nitrososphaera which is related to ammonia oxidation process, and it would have a promising potential as soil amendment to reduce nitrogen loss probably.


Soil ABC/NZVI Arsenic Rice Microbial diversity Rhizosphere 



This study was funded by the National High Technology Research and Development Program of China (2013AA06A209) and the Science and Technology Planning Project of Guangdong Province, China (2016B020242004).


  1. Atkinson CJ, Fitzgerald JD, Hipps NA (2010) Potential mechanisms for achieving agricultural benefits from biochar application to temperate soils: a review. Plant Soil 337:1–18CrossRefGoogle Scholar
  2. Bian R, Chen D, Liu X, Cui L, Li L, Pan G, Xie D, Zheng J, Zhang X, Zheng J, Chang A (2013) Biochar soil amendment as a solution to prevent Cd-tainted rice from China: results from a cross-site field experiment. Ecol Eng 58:378–383CrossRefGoogle Scholar
  3. Bian R, Joseph S, Cui L, Pan G, Li L, Liu X, Zhang A, Rutlidge H, Wong S, Chia C, Marjo C, Gong B, Munroe P, Donne S (2014) A three-year experiment confirms continuous immobilization of cadmium and lead in contaminated paddy field with biochar amendment. J Hazard Mater 272:121–128CrossRefGoogle Scholar
  4. Bostick BC, Chen C, Fendorf S (2004) Arsenite retention mechanisms within estuarine sediments of Pescadero, CA. Environ Sci Technol 38:3299–3304CrossRefGoogle Scholar
  5. Bostick BC, Fendorf S (2003) Arsenite sorption on troilite (FeS) and pyrite (FeS2). Geochim Cosmochim Acta 67:909–921CrossRefGoogle Scholar
  6. Buttry DA, Peng JCM, Donnet J-B, Rebouillat S (1999) Immobilization of amines at carbon fiber surfaces. Carbon 37:1929–1940CrossRefGoogle Scholar
  7. Chan KY, Van Zwieten L, Meszaros I, Downie A, Joseph S (2007) Agronomic values of greenwaste biochar as a soil amendment. Soil Res 45:629–634CrossRefGoogle Scholar
  8. Childers SE, Ciufo S, Lovley DR (2002) Geobacter metallireducens accesses insoluble Fe(III) oxide by chemotaxis. Nature 416:767–769CrossRefGoogle Scholar
  9. China, N. S. (1995) Environmental quality standard for soils. GB 15618-1995. National Environmental Protection Agency of ChinaGoogle Scholar
  10. Driehaus W, Jekel M, Hildebrandt U (1998) Granular ferric hydroxide—a new adsorbent for the removal of arsenic from natural water. J Water Supply Res Technol AQUA 47:30–35Google Scholar
  11. Fellet G, Marchiol L, Delle Vedove G, Peressotti A (2011) Application of biochar on mine tailings: effects and perspectives for land reclamation. Chemosphere 83:1262–1267CrossRefGoogle Scholar
  12. Fendorf S, Michael HA, van Geen A (2010) Spatial and temporal variations of groundwater arsenic in south and southeast Asia. Science 328:1123–1127CrossRefGoogle Scholar
  13. Gao Y, Mucci A (2003) Individual and competitive adsorption of phosphate and arsenate on goethite in artificial seawater. Chem Geol 199:91–109CrossRefGoogle Scholar
  14. Glaser B, Haumaier L, Guggenberger G, Zech W (2001) The ‘Terra Preta’ phenomenon: a model for sustainable agriculture in the humid tropics. Naturwissenschaften 88:37–41CrossRefGoogle Scholar
  15. Glaser B, Lehmann J, Zech W (2002) Ameliorating physical and chemical properties of highly weathered soils in the tropics with charcoal—a review. Biol Fertil Soils 35:219–230CrossRefGoogle Scholar
  16. Guan X-H, Wang J, Chusuei CC (2008) Removal of arsenic from water using granular ferric hydroxide: macroscopic and microscopic studies. J Hazard Mater 156:178–185CrossRefGoogle Scholar
  17. Guo X, Chen F (2005) Removal of arsenic by bead cellulose loaded with iron oxyhydroxide from groundwater. Environ Sci Technol 39:6808–6818CrossRefGoogle Scholar
  18. Handler RM, Frierdich AJ, Johnson CM, Rosso KM, Beard BL, Wang C, Latta DE, Neumann A, Pasakarnis T, Premaratne WAPJ, Scherer MM (2014) Fe(II)-catalyzed recrystallization of goethite revisited. Environ Sci Technol 48:11302–11311CrossRefGoogle Scholar
  19. Hansel CM, Benner SG, Fendorf S (2005) Competing Fe(II)-induced mineralization pathways of ferrihydrite. Environ Sci Technol 39:7147–7153CrossRefGoogle Scholar
  20. Hossain MK, Strezov V, Yin Chan K, Nelson PF (2010) Agronomic properties of wastewater sludge biochar and bioavailability of metals in production of cherry tomato (Lycopersicon esculentum). Chemosphere 78:1167–1171CrossRefGoogle Scholar
  21. Houben D, Evrard L, Sonnet P (2013) Beneficial effects of biochar application to contaminated soils on the bioavailability of Cd, Pb and Zn and the biomass production of rapeseed (Brassica napus L.) Biomass Bioenergy 57:196–204CrossRefGoogle Scholar
  22. Jeon B-H, Dempsey BA, Burgos WD (2003) Kinetics and mechanisms for reactions of Fe(II) with iron(III) oxides. Environ Sci Technol 37:3309–3315CrossRefGoogle Scholar
  23. Khan S, Chao C, Waqas M, Arp HPH, Zhu Y-G (2013) Sewage sludge biochar influence upon rice (Oryza sativa L) yield, metal bioaccumulation and greenhouse gas emissions from acidic paddy soil. Environ Sci Technol 47:8624–8632CrossRefGoogle Scholar
  24. Latta DE, Gorski CA, Scherer MM (2012) Influence of Fe2+-catalysed iron oxide recrystallization on metal cycling. Biochem Soc Trans 40:1191–1197CrossRefGoogle Scholar
  25. Lehmann J (2007) A handful of carbon. Nature 447:143–144CrossRefGoogle Scholar
  26. Lehmann J, Gaunt J, Rondon M (2006) Bio-char sequestration in terrestrial ecosystems—a review. Mitig Adapt Strateg Glob Chang 11:395–419CrossRefGoogle Scholar
  27. Leininger S, Urich T, Schloter M, Schwark L, Qi J, Nicol GW, Prosser JI, Schuster SC, Schleper C (2006) Archaea predominate among ammonia-oxidizing prokaryotes in soils. Nature 442:806–809CrossRefGoogle Scholar
  28. Liu C-P, Luo C-L, Gao Y, Li F-B, Lin L-W, Wu C-A, Li X-D (2010) Arsenic contamination and potential health risk implications at an abandoned tungsten mine, southern China. Environ Pollut 158:820–826CrossRefGoogle Scholar
  29. Liu C-P, Luo C-L, Xu X-H, Wu C-A, Li F-B, Zhang G (2012) Effects of calcium peroxide on arsenic uptake by celery (Apium graveolens L.) grown in arsenic contaminated soil. Chemosphere 86:1106–1111CrossRefGoogle Scholar
  30. Mann CC (2002) The real dirt on rainforest fertility. Science 297:920–923CrossRefGoogle Scholar
  31. Méndez A, Gómez A, Paz-Ferreiro J, Gascó G (2012) Effects of sewage sludge biochar on plant metal availability after application to a Mediterranean soil. Chemosphere 89:1354–1359CrossRefGoogle Scholar
  32. O'Dell R, Silk W, Green P, Claassen V (2007) Compost amendment of Cu–Zn minespoil reduces toxic bioavailable heavy metal concentrations and promotes establishment and biomass production of Bromus carinatus (Hook and Arn.) Environ Pollut 148:115–124CrossRefGoogle Scholar
  33. Pester M, Rattei T, Flechl S, Gröngröft A, Richter A, Overmann J, Reinhold-Hurek B, Loy A, Wagner M (2012) amoA-based consensus phylogeny of ammonia-oxidizing archaea and deep sequencing of amoA genes from soils of four different geographic regions. Environ Microbiol 14:525–539CrossRefGoogle Scholar
  34. Su H, Fang Z, Tsang PE, Zheng L, Cheng W, Fang J, Zhao D (2016) Remediation of hexavalent chromium contaminated soil by biochar-supported zero-valent iron nanoparticles. J Hazard Mater 318:533–540CrossRefGoogle Scholar
  35. Su Y-H, McGrath SP, Zhao F-J (2010) Rice is more efficient in arsenite uptake and translocation than wheat and barley. Plant Soil 328:27–34CrossRefGoogle Scholar
  36. Tosco T, Petrangeli Papini M, Cruz Viggi C, Sethi R (2014) Nanoscale zerovalent iron particles for groundwater remediation: a review. J Clean Prod 77:10–21CrossRefGoogle Scholar
  37. Tourna M, Stieglmeier M, Spang A, Könneke M, Schintlmeister A, Urich T, Engel M, Schloter M, Wagner M, Richter A, Schleper C (2011) Nitrososphaera viennensis, an ammonia oxidizing archaeon from soil. Proc Natl Acad Sci U S A 108:8420–8425CrossRefGoogle Scholar
  38. Van Herreweghe S, Swennen R, Vandecasteele C, Cappuyns V (2003) Solid phase speciation of arsenic by sequential extraction in standard reference materials and industrially contaminated soil samples. Environ Pollut 122:323–342CrossRefGoogle Scholar
  39. Vogel, A. I., and J. Mendham (1989) Vogel's textbook of quantitative chemical analysis. Longman Scientific and Technical. John wiley, New YorkGoogle Scholar
  40. Wang Z-X, Hu X-B, Xu Z-C, Cai L-M, Wang J-N, Zeng D, Hong H-J (2014) Cadmium in agricultural soils, vegetables and rice and potential health risk in vicinity of Dabaoshan Mine in Shaoguan, China. J Cent South Univ 21:2004–2010CrossRefGoogle Scholar
  41. Wang N, Xue X-M, Juhasz AL, Chang Z-Z, Li H-B (2017) Biochar increases arsenic release from an anaerobic paddy soil due to enhanced microbial reduction of iron and arsenic. Environmental Pollution 220(Part A):514–522CrossRefGoogle Scholar
  42. Waychunas GA, Kim CS, Banfield JF (2005) Nanoparticulate iron oxide minerals in soils and sediments: unique properties and contaminant scavenging mechanisms. J Nanopart Res 7:409–433CrossRefGoogle Scholar
  43. Williams PN, Villada A, Deacon C, Raab A, Figuerola J, Green AJ, Feldmann J, Meharg AA (2007) Greatly enhanced arsenic shoot assimilation in rice leads to elevated grain levels compared to wheat and barley. Environ Sci Technol 41:6854–6859CrossRefGoogle Scholar
  44. Wu C, Ye Z, Li H, Wu S, Deng D, Zhu Y, Wong M (2012) Do radial oxygen loss and external aeration affect iron plaque formation and arsenic accumulation and speciation in rice? J Exp Bot 63:2961–2970CrossRefGoogle Scholar
  45. Xu P, Wang Z (2014) A comparison study in cadmium tolerance and accumulation in two cool-season turfgrasses and Solanum nigrum L. Water Air Soil Pollut 225:1938CrossRefGoogle Scholar
  46. Yang G-X, Jiang H (2014) Amino modification of biochar for enhanced adsorption of copper ions from synthetic wastewater. Water Res 48:396–405CrossRefGoogle Scholar
  47. Yang JE, Kim HJ, Ok Y-S, Lee J-Y, Park J (2007) Treatment of abandoned coal mine discharged waters using lime wastes. Geosci J 11:111–114CrossRefGoogle Scholar
  48. Yang, C., T. X. Ma, Z. Zhang, Z. Dang., C. L. Guo, G. N. Lu, and X. Y. Yi (2016) Iron-based amino composite modified charcoal material as well as preparation and application. in S. C. U. o. Technology, editor. State Intellectual Property Office, ChinaGoogle Scholar
  49. Zhu H, Jia Y, Wu X, Wang H (2009) Removal of arsenic from water by supported nano zero-valent iron on activated carbon. J Hazard Mater 172:1591–1596CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Shusi Liu
    • 1
  • Yixin Lu
    • 1
  • Chen Yang
    • 1
    • 2
    • 3
  • Chuanping Liu
    • 4
  • Lin Ma
    • 1
  • Zhi Dang
    • 1
  1. 1.College of Environment and EnergySouth China University of TechnologyGuangzhouPeople’s Republic of China
  2. 2.The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of EducationSouth China University of Technology, Guangzhou Higher Education Mega CentreGuangzhouPeople’s Republic of China
  3. 3.Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency DisposalSouth China University of Technology, Guangzhou Higher Education Mega CentreGuangzhouPeople’s Republic of China
  4. 4.Guangdong Institute of Eco-Environmental and Soil SciencesGuangzhouPeople’s Republic of China

Personalised recommendations