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Possible protective effect of the algae spirulina against nephrotoxicity induced by cyclosporine A and/or gamma radiation in rats

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Abstract

The present study was conducted to evaluate the possible protective role of the algae spirulina (Sp) against nephrotoxicity and oxidative stress which are the main secondary effects induced by the immunosuppressant drug CSA and/or ionizing radiation. In this study, male rats were given Sp (1 g/kg) either for 15 days before irradiation (6.5 Gy) or 5 days before and 10 days concomitant with CSA (25 mg/kg). Markers used to assess renal injury included serum creatinine, urea, glucose, albumin, protein, and lipid profile as well as kidney content of reduced glutathione (GSH); lipid peroxidation (thiobarbituric acid reactive substances (TBARS)); nitrite and superoxide dismutase (SOD) activity. In addition, some trace elements (Zn and Mg) were estimated in kidney. Apoptosis was assessed by immunohistochemical estimation of caspase-3 expression in addition to histopathological examination. Results revealed that gamma radiation and/or CSA induced elevation in urea, creatinine, lipids, and glucose while decreasing albumin and protein levels. There was a noticeable increase in kidney content of GSH, TBARS, and nitrite. Meanwhile, profound decrease in kidney SOD activity was observed. Treatment with Sp significantly reversed the changes induced by CSA and/or gamma radiation in renal function tests. Spirulina also ameliorated kidney oxidative stress through decreasing GSH, TBARS, and nitrite kidney content while increasing SOD activity. Histopathological examination further confirmed Sp protective efficacy. Moreover, kidney caspase-3 expression that was triggered by CSA and/or gamma radiation was decreased. In conclusion, spirulina can be regarded as a promising renoprotective natural agent against renal injury induced by CSA and/or gamma radiation.

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Acknowledgements

The authors gratefully acknowledge the help of Dr. Sahar Samir Abdel-Rahman, Professor of Pathology, Faculty of Veterinary Medicine, Cairo University, for carrying out the histological examinations.

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Authors and Affiliations

  1. Department of Drug Radiation Research, National Center for Radiation Research and Technology, Atomic Energy Authority, Nasr City, PO Box 29, Cairo, Egypt

    Maha M. Aziz, Ahmed S. Nada & Nour El-Din Amin

  2. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Giza, Egypt

    Nihad I. Eid & Afaf A. Ain-Shoka

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  1. Maha M. Aziz
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  2. Nihad I. Eid
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Correspondence to Maha M. Aziz.

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The study was conducted in accordance with the guidelines set by the EEC regulations (Revised Directive 86/609/EEC) and approved by the Ethics Committee for Experimental and Clinical Studies at Faculty of Pharmacy, Cairo University (PT.1086).

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Aziz, M.M., Eid, N.I., Nada, A.S. et al. Possible protective effect of the algae spirulina against nephrotoxicity induced by cyclosporine A and/or gamma radiation in rats. Environ Sci Pollut Res 25, 9060–9070 (2018). https://doi.org/10.1007/s11356-017-1146-0

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