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Analysis of various effects of abamectin on erythrocyte morphology in Japanese quails (Coturnix japonica)

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Abstract

Although previous studies have already confirmed the toxicological potential of abamectin (ABA) in different experimental models (from invertebrates to vertebrates), almost nothing is known about the impacts the exposure to this pesticide can cause on birds. Thus, the aim of our study is to investigate the cytotoxic effects on the erythrocytes of female Japanese quails (Coturnix japonica) exposed to low abamectin concentrations. In order to do so, three experimental groups were proposed: “control,” composed of quails exposed to abamectin-free drinking water; “ABA 1% median lethal dose (LD50),” comprising birds exposed to water containing 15.5 mg a.i./L of abamectin (via commercial formulation Kraft® 36EC), and “ABA 10% LD50,” composed by birds exposed to water containing 155.0 mg a.i./L of abamectin. The micronucleus test and the tests applied to other nuclear abnormalities in the peripheral blood of birds were conducted 40 days after exposure. Our study revealed significant physical abnormalities in nuclear shapes (erythrocytes with asymmetric constriction nuclei, notched nuclei, indented and moved nucleus) of those birds exposed to higher abamectin levels. When all nuclear abnormalities were tallied, a significant dose-dependent trend was noted. Therefore, our study presents initial imprints on determination of abamectin-mediated cellular toxicity in avifauna which can be instrumental in checking polluted ecosystems.

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Acknowledgments

The authors are grateful to the Brazilian National Council for Research (CNPq) (Brazilian research agency) (Proc. No. 467801/2014-2) and Instituto Federal Goiano for the financial support. Moreover, the authors are grateful to the CNPq for supporting scholarship to the student who developed this study.

Funding

This study was funded by the Brazilian National Council for Research (CNPq) (Brazilian research agency) (Proc. No. 467801/2014-2) and Instituto Federal Goiano – Campus Urutaí (GO, Brazil).

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Authors and Affiliations

  1. Post-Graduation Program in Conservation of Natural Resources in Cerrado, Goiano Federal Institute of Education, Science and Technology – Urutaí Campus, Urutaí, GO, Brazil

    Denise Braga Gomes de Faria, Mateus Flores Montalvão, Joyce Moreira de Souza, Bruna de Oliveira Mendes, Guilherme Malafaia & Aline Sueli de Lima Rodrigues

  2. Laboratório de Pesquisas Biológicas, Instituto Federal Goiano – Campus Urutaí, Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, 75790-000, Brazil

    Guilherme Malafaia

  3. Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, CEP: 75790-000, Brazil

    Guilherme Malafaia

Authors
  1. Denise Braga Gomes de Faria
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  2. Mateus Flores Montalvão
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  3. Joyce Moreira de Souza
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  4. Bruna de Oliveira Mendes
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  5. Guilherme Malafaia
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  6. Aline Sueli de Lima Rodrigues
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Corresponding author

Correspondence to Guilherme Malafaia.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All the procedures were approved by The Ethics Committee on Animal Use of Goiano Federal Institute (Comissão de Ética no Uso de Animais do Instituto Federal Goiano), GO, Brazil (protocol No. 7257130516). Meticulous efforts were made to assure that the animals suffered the least possible and to reduce external sources of stress, pain, and discomfort. The current study did not exceed the number of animals necessary to produce trustworthy scientific data. This article does not contain any studies with human participants performed by any of the authors.

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Responsible editor: Philippe Garrigues

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de Faria, D.B.G., Montalvão, M.F., de Souza, J.M. et al. Analysis of various effects of abamectin on erythrocyte morphology in Japanese quails (Coturnix japonica). Environ Sci Pollut Res 25, 2450–2456 (2018). https://doi.org/10.1007/s11356-017-0677-8

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  • DOI: https://doi.org/10.1007/s11356-017-0677-8

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