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Evaluating genotoxic risks in Brazilian public health agents occupationally exposed to pesticides: a multi-biomarker approach

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Abstract

This is the first study demonstrating genotoxic effects and whole transcriptome analysis on community health agents (CHAs) occupationally exposed to pesticides in Central Brazil. For the transcriptome analysis, we found some genes related to Alzheimer’s disease (LRP1), an insulin-like growth factor receptor (IGF2R), immunity genes (IGL family and IGJ), two genes related to inflammatory reaction (CXCL5 and CCL3), one gene related to maintenance of cellular morphology (NHS), one gene considered to be a strong apoptosis inductor (LGALS14), and several transcripts of the neuroblastoma breakpoint family (NBPF). Related to comet assay, we demonstrated a significant increase in DNA damage, measured by the olive tail moment (OTM), in the exposed group compared to the control group. Moreover, we also observed a statistically significant difference in OTM values depending on GSTM1 genotypes. Therefore, Brazilian epidemiological surveillance, an organization responsible for the assessment and management of health risks associated to pesticide exposure to CHA, needs to be more proactive and considers the implications of pesticide exposure for CHA procedures and processes.

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Acknowledgments

The authors acknowledged Mr. R. Smith for proofreading this manuscript. Fundação de Amparo à Pesquisa do Estado de Goiás and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Edital Universal-2012, Proc. 475418/2012-3) are also acknowledged. RPB and ADC thank CNPq fellowship. AAA thanks FAPEG for fellowship. FCF, FRG, WFC, and TBV thank CAPES for their fellowship. We thank the Brazilian Community Health Agents, their supervisor, Mr. I. Silva, and the non-exposed population for participating voluntarily in this research.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Genética e Biologia Molecular, Laboratório de Mutagênese e Radiobiologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil

    Fernanda Craveiro Franco, Alessandro Arruda Alves, Juliana Boaventura Avelar, Thays Millena Alves Pedroso & Daniela de Melo e Silva

  2. Núcleo de Pesquisas Replicon, Departamento de Biologia, Pontifícia Universidade Católica de Goiás, Goiânia, Goiás, Brazil

    Fernanda Ribeiro Godoy, Douglas Dantas Rodrigues & Aparecido Divino da Cruz

  3. Campus II, Itatiaia, Departamento de Genética, Instituto de Ciências Biológicas 1, Universidade Federal de Goiás, Goiânia, Goiás, Cep 74001-970, Brazil

    Fausto Nomura & Daniela de Melo e Silva

  4. Programa de Pós-Graduação em Ciências Ambientais, Laboratório de Mutagênese e Radiobiologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil

    Daniela de Melo e Silva

  5. Programa de Pós-Graduação em Biodiversidade Animal, Universidade Federal de Goiás, Goiânia, Goiás, Brazil

    Daniela de Melo e Silva

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  1. Fernanda Craveiro Franco
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  2. Alessandro Arruda Alves
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  3. Fernanda Ribeiro Godoy
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  4. Juliana Boaventura Avelar
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  5. Douglas Dantas Rodrigues
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  6. Thays Millena Alves Pedroso
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  7. Aparecido Divino da Cruz
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  8. Fausto Nomura
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  9. Daniela de Melo e Silva
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Correspondence to Daniela de Melo e Silva.

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Franco, F.C., Alves, A.A., Godoy, F.R. et al. Evaluating genotoxic risks in Brazilian public health agents occupationally exposed to pesticides: a multi-biomarker approach. Environ Sci Pollut Res 23, 19723–19734 (2016). https://doi.org/10.1007/s11356-016-7179-y

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