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Assessment of Toxoplasma gondii levels in zebra mussel (Dreissena polymorpha) by real-time PCR: an organotropism study

  • Microbial Ecology of the Continental and Coastal Environments
  • Published:
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Abstract

Water quality is a public health concern that calls for relevant biomonitoring programs. Molecular tools such as polymerase chain reaction (PCR) are progressively becoming more sensitive and more specific than conventional techniques to detect pathogens in environmental samples such as water and organisms. The zebra mussel (Dreissena polymorpha) has already been demonstrated to accumulate and concentrate various human waterborne pathogens. In this study, first, a spiking experiment to evaluate detection levels of Toxoplasma gondii DNA in zebra mussel organs using real-time PCR was conducted. Overall, lower DNA levels in the hemolymph, digestive gland, and remaining tissues (gonad and foot) were detected compared to mantle, muscle, and gills. Second, an in vivo experiment with 1000 T. gondii oocysts per mussel and per day for 21 consecutive days, followed by 14 days of depuration time in protozoa-free water was performed. T. gondii DNA was detected in all organs, but greatest concentrations were observed in hemolymph and mantle tissues compared to the others organs at the end of the depuration period. These results suggest that (i) the zebra mussel is a potential new tool for measuring T. gondii concentrations and (ii) real-time PCR is a suitable method for pathogen detection in complex matrices such as tissues.

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Acknowledgments

This Ph.D. work was supported by grants from the “Région Champagne-Ardenne” (projet INTERBIO). Financial support was provided by the CNRS-INSU (Programme EC2CO, projet IPAD) and the Programme Interdisciplinaire de Recherche sur l’Environnement de la Seine (PIREN-Seine). The authors thank Stéphanie La Carbona and Catherine Cazeaux, ACTALIA, Villers Bocage, France, for optimizing the real-time quantitative PCR procedure. We are grateful to Annie Buchwalter for the English revision.

Ethical considerations

In France, experiments conducted with Dreissena polymorpha do not require authorization. This specie is not protected and their use in scientific research does not require a specific permit. However, every effort will be made to reduce the suffering of animals.

Conflict of interest

The authors declare no competing conflict of interest.

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Authors and Affiliations

  1. Unité Stress Environnementaux et BIOsurveillance des milieux aquatiques, UMR-I 02 (SEBIO), Université de Reims Champagne-Ardenne, Reims, France

    M. Palos Ladeiro, A. Bigot-Clivot & A. Geffard

  2. Laboratoire de Parasitologie-Mycologie, EA 3800, SFR CAP-Santé FED 4231, Hôpital Maison Blanche, Reims, France

    M. Palos Ladeiro, D. Aubert & I. Villena

Authors
  1. M. Palos Ladeiro
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  2. A. Bigot-Clivot
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  3. D. Aubert
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  4. I. Villena
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  5. A. Geffard
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Corresponding author

Correspondence to A. Bigot-Clivot.

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Responsible editor: Markus Hecker

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Palos Ladeiro, M., Bigot-Clivot, A., Aubert, D. et al. Assessment of Toxoplasma gondii levels in zebra mussel (Dreissena polymorpha) by real-time PCR: an organotropism study. Environ Sci Pollut Res 22, 13693–13701 (2015). https://doi.org/10.1007/s11356-015-4296-y

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  • DOI: https://doi.org/10.1007/s11356-015-4296-y

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