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Three major nucleolar proteins migrate from nucleolus to nucleoplasm and cytoplasm in root tip cells of Vicia faba L. exposed to aluminum

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Abstract

Results from our previous investigation indicated that Al could affect the nucleolus and induce extrusion of silver-staining nucleolar particles containing argyrophilic proteins from the nucleolus into the cytoplasm in root tip cells of Vicia faba L. So far, the nucleolar proteins involved have not been identified. It is well known that nucleophosmin (B23), nucleolin (C23), and fibrillarin are three major and multifunctional nucleolar proteins. Therefore, effects of Al on B23, C23, and fibrillarin in root tip cells of V. faba exposed to 100 μM Al for 48 h were observed and analyzed using indirect immunofluorescence microscopy and Western blotting. The results from this work demonstrated that after 100 μM of Al treatment for 48 h, B23 and C23 migrated from the nucleolus to the cytoplasm and fibrillarin from the nucleolus to the nucleoplasm. In some cells, fibrillarin was present only in the cytoplasm. Western blotting data revealed higher expression of the three major nucleolar proteins in Al-treated roots compared with the control and that the B23 content increased markedly. These findings confirmed our previous observations.

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Acknowledgments

This project was supported by the National Natural Science Foundation of China (#30972331) and the Scientific Research Foundation of Graduate School of South China Normal University (C1045210). The authors wish to express their appreciation to the reviewers for this paper. The authors are grateful to Professor Lars Olof Björn (South China Normal University) for critical reading of the manuscript and Professor Helen Ghiradella, The University at Albany, for language improvement.

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Authors and Affiliations

  1. Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, 510631, Guangzhou, People’s Republic of China

    Rong Qin & Shaoshan Li

  2. Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, 300387, Tianjin, People’s Republic of China

    Rong Qin, Huaning Zhang, Wusheng Jiang & Donghua Liu

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  1. Rong Qin
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  2. Huaning Zhang
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  3. Shaoshan Li
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  4. Wusheng Jiang
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  5. Donghua Liu
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Correspondence to Donghua Liu.

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Responsible editor: Elena Maestri

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Qin, R., Zhang, H., Li, S. et al. Three major nucleolar proteins migrate from nucleolus to nucleoplasm and cytoplasm in root tip cells of Vicia faba L. exposed to aluminum. Environ Sci Pollut Res 21, 10736–10743 (2014). https://doi.org/10.1007/s11356-014-3057-7

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  • DOI: https://doi.org/10.1007/s11356-014-3057-7

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