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EDTA-enhanced phytoremediation of lead-contaminated soil by the halophyte Sesuvium portulacastrum

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Abstract

The low bioavailability of Pb and low number of Pb-tolerant plant species represent an important limitation for Pb phytoextraction. It was recently suggested that halophyte plant species may be a promising material for this purpose, especially in polluted salt areas while Pb mobility may be improved by synthetic chelating agents. This study aims to evaluate Pb extraction by the halophyte Sesuvium portulacastrum in relation to the impact of EDTA application. Seedling were cultivated during 60 days on Pb artificially contaminated soil (200, 400, and 800 ppm Pb) in the presence or in the absence of EDTA (3 g kg−1 soil). Results showed that upon to 400 ppm, Pb had no impact on plant growth. However, exogenous Pb induce a decrease in shoot K+ while it increased shoot Mg2+ and had no impact on shoot Ca2+ concentrations. Lead concentration in the shoots increased with increasing external Pb doses reaching 1,390 ppm in the presence of 800 ppm lead in soil. EDTA addition had no effect on plant growth but strongly increased Pb accumulation in the shoot which increased from 1,390 ppm in the absence of EDTA to 3,772 ppm in EDTA-amended plants exposed to 800 ppm exogenous Pb. Both Pb absorption and translocation from roots to shoots were significantly enhanced by EDTA application, leading to an increase in the total amounts of extracted Pb per plant. These data suggest that S. portulacastrum is very promising species for decontamination of Pb2+-contaminated soil and that its phytoextraction potential was significantly enhanced by addition of EDTA to the polluted soil.

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Acknowledgments

This work was conducted in the Laboratory of Extremophile Plants in the Biotechnology Center of Borj Cedria Tunisia and supported by the Tunisian Ministry of Higher Education and Scientific Research (LR10CBBC02).

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Authors and Affiliations

  1. Laboratoire des Plantes Extremophiles, Centre de Biotechnologie de Borj-Cédria, BP 901, Hammam Lif, 2050, Tunisia

    Hanen Zaier, Tahar Ghnaya, Rim Ghabriche, Abelbasset Lakhdar & Chedly Abdelly

  2. Laboratoire de chimie Eaux-Sols-Boues, Institut National de Recherche en Génie Rurale, Eaux et Forêts, Tunis, Tunisia

    Walid Chmingui

  3. Institut Préparatoire aux Études d’Ingénieurs de Sfax, Menzel Chaker km 0,5, 3018, Sfax, Tunisia

    Abelbasset Lakhdar

  4. Groupe de Recherche en Physiologie végétale, Earth and Life Institute – Agronomy, Université Catholique de Louvain, 1348, Louvain-la-Neuve, Belgium

    Stanley Lutts

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  1. Hanen Zaier
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  2. Tahar Ghnaya
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Correspondence to Hanen Zaier.

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Responsible editor: Elena Maestri

Both authors Hanen Zaier and Tahar Ghnaya equally contributed to this work.

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Zaier, H., Ghnaya, T., Ghabriche, R. et al. EDTA-enhanced phytoremediation of lead-contaminated soil by the halophyte Sesuvium portulacastrum . Environ Sci Pollut Res 21, 7607–7615 (2014). https://doi.org/10.1007/s11356-014-2690-5

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