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Changes of metal-induced toxicity by H2O2/NO modulators in Scenedesmus quadricauda (Chlorophyceae)

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Abstract

Effect of nitric oxide donor (sodium nitroprusside, SNP, 500 μM) or hydrogen peroxide scavenger (dithiothreitol, DTT, 500 μM) on cadmium (Cd) or copper (Cu) uptake (150 μM solutions) and toxicity using Scenedesmus quadricauda was studied. Combined treatments (Cd or Cu + DTT or SNP) usually ameliorated metal-induced toxicity at the level of pigments, proteins, and mineral nutrients in comparison with respective metal alone. Viability tests (MTT and TTC) showed the lowest values preferentially in Cu treatments, indicating higher toxicity in comparison with Cd. Cd showed low impact on amino acids while strong Cu-induced depletion was mitigated by DTT and SNP. Amount of ROS and NO showed the most pronounced responses in SNP variants being rather reciprocal than parallel and regulated ascorbate peroxidase activity. Blot gel analyses of hsp70 protein did not reveal extensive changes after given exposure period. Phenols were elevated by DTT alone while all Cu treatments revealed depletion. Total Cu content decreased while total Cd content increased in metal + SNP or metal + DTT. Subsequent experiment using lower Cd, SNP or DTT doses (10 and 100 μM) revealed concentration-dependent impact on Cd uptake. Overall, DTT was found to be more suitable for the amelioration of metal-induced toxicity.

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Acknowledgments

This work was supported by the Slovak Grant Agency VEGA (1/1238/12) and the CEITEC—Central European Institute of Technology with research infrastructure supported by the project CZ.1.05/1.1.00/02.0068 financed from European Regional Development Fund. The authors thank Dr. Jaromír Mikeš for help with the assay of ROS by flow cytometry.

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The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Department of Botany, Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 041 67, Košice, Slovak Republic

    František Štork & Martin Bačkor

  2. Institute of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00, Brno, Czech Republic

    Bořivoj Klejdus, Josef Hedbavny & Jozef Kováčik

  3. CEITEC—Central European Institute of Technology, Mendel University in Brno, Zemědělská 1, 613 00, Brno, Czech Republic

    Bořivoj Klejdus & Jozef Kováčik

Authors
  1. František Štork
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  2. Martin Bačkor
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  3. Bořivoj Klejdus
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  4. Josef Hedbavny
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  5. Jozef Kováčik
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Correspondence to Jozef Kováčik.

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Responsible editor: Philippe Garrigues

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Štork, F., Bačkor, M., Klejdus, B. et al. Changes of metal-induced toxicity by H2O2/NO modulators in Scenedesmus quadricauda (Chlorophyceae). Environ Sci Pollut Res 20, 5502–5511 (2013). https://doi.org/10.1007/s11356-013-1541-0

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  • DOI: https://doi.org/10.1007/s11356-013-1541-0

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