Abstract
In vitro digestion test can be applied to evaluate the bioaccessibility of soil metals by measuring the solubility of the metals in synthetic human digestive tract. Physiologically based extraction test (PBET), composed of sequential digestion of gastric and intestinal phase, is one of the frequently used in vitro digestion tests. In this study, the PBET was chosen to determine the bioaccessibility of Cu, Zn, and Pb in 14 mildly acidic and alkali (pH 5.87–8.30) soils. The phytoavailability of Cu, Zn, and Pb in the same soils was also measured using six single-extraction methods (0.1 M HNO3, 0.4 M HOAc, 0.1 M NaNO3, 0.01 M CaCl2, 0.05 M EDTA, and 0.5 M DTPA). The extraction efficiencies of the methods were compared. The PBET had a strong ability to extract metals from soil, which was much greater than neutral salt extraction and close to dilute acid and complex extraction in spite of the last 2 h neutral intestinal digestion. The amounts of bioaccessible Cu, Zn, and Pb in the gastric phase and in the gastrointestinal phase were both largely determined by the total content of soil Cu, Zn, and Pb. But the results of gastrointestinal digestion reflected more differences resulting from element and soil types than those of gastric digestion did. It was noticed that most of variations in the amounts of soil Cu, Zn, and Pb extracted by EDTA were well explained by the total soil Cu, Zn, and Pb, as same as the PBET. Moreover, the solubility of Cu, Zn, and Pb in the gastric phase and gastrointestinal phase were all positively linearly correlated with the results of EDTA. It was suggested that EDTA extraction can be used to predict the bioaccessibility of Cu, Zn, and Pb in mildly acidic and alkali (pH > 5.8) soils, and the PBET and EDTA could be applied to measure, in a certain extent, the bioaccessibility and phytoavailability of Cu, Zn, and Pb in mildly acidic and alkali (pH > 5.8) soils at the same time.
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References
Basta N, Gradwohl R (2000) Estimation of Cd, Pb, and Zn bioavailability in smelter-contaminated soils by a sequential extraction procedure. J Soil Contam 9(2):149–164
Cao X, Wahbi A, Ma L et al (2009) Immobilization of Zn, Cu, and Pb in contaminated soils using phosphate rock and phosphoric acid. J Hazard Mater 164(2–3):555–564
Carrizales L, Razo I, Tellez-Hernandez JI et al (2006) Exposure to arsenic and lead of children living near a copper-smelter in San Luis Potosi, Mexico: importance of soil contamination for exposure of children. Environ Res 101:1–10
Cave M, Wragg J, Klinck B et al (2006) Preliminary assessment of a unified bioaccessibility method for potentially harmful elements in soils. Epidemiology 17:39
Cieslinski G, Van Rees KCJ, Szmigielska AM et al (1998) Low-molecular-weight organic acids in rhizosphere soils of durum wheat and their effect on cadmium bioaccumulation. Plant Soil 203:109–117
Dean JR, Ma R (2007) Approaches to assess the oral bioaccessibility of persistent organic pollutants: a critical review. Chemosphere 68:1399–1407
Denys S, Caboche J, Tack K et al (2012) In vivo validation of the unified BARGE method to assess the bioaccessibility of arsenic, antimony, cadmium, and lead in soils. Environ Sci Technol 46:6252–6260
Dickman SR, Bray RH (1940) Colorimetric determination of phosphate. Ind Eng Chem Res 12(11):665–668
Fan J, He Z, Ma LQ, Stoffella PJ (2011a) Accumulation and availability of copper in citrus grove soils as affected by fungicide application. J Soil Sediments 11:639–648
Fan J, He Z, Ma LQ, Yang Y, Yang X, Stoffella PJ (2011b) Immobilization of copper in contaminated sandy soils using calcium water treatment residue. J Hazard Mater 189:710–718
Fan J, He Z, Ma LQ, Nogueira TAR, Wang Y, Liang Z, Stoffella PJ (2012) Calcium water treatment residue reduces copper phytotoxicity in contaminated sandy soils. J Hazard Mater 199–200:375–382
Florido MC, Madrid F, Ajmone-Marsan F (2011) Variations of metal availability and bio-accessibility in water-logged soils with various metal contents: in vitro experiments. Water Air Soil Pollut 217:149–156
Freeman GB, Johnson JD, Liao SC, Davis AO, Ruby MV, Schoof RA, Chaney RL, Bergstrom PD (1994) Absolute bioavailability of lead acetate and mining waste lead in rats. Toxicology 91:151–163
Freeman GB, Dill JA, Johnson JD, Kurtz PJ, Parham F, Matthews HB (1996) Comparative absorption of lead from contaminated soil and lead salts by weanling Fischer 344 rats. Fundam Appl Toxicol 33:109–119
Gupta SK, Aten C (1993) Comparison and evaluation of extraction media and their suitability in a simple model to predict the biological relevance of heavy metal concentrations in contaminated soils. Int J Environ Anal Chem 51:25–46
Houba VJG, Novozamsky I, Huybregets AWM et al (1986) Comparison of soil extractions by 0.01 M CaCl2, by EUF and by some conventional extraction procedures. Plant Soil 96:433–437
Houba VJG, Lexmond Th M, Novozamsky I et al (1996) State of the art and future developments in soil analysis for bioavailability assessment. Sci Total Environ 178:21–28
Intawongse M, Dean JR (2006) In-vitro testing for assessing oral bioaccessibility of trace metals in soil and food samples. TrAc-Trend Anal Chem 25:876–886
Intawongse M, Dean JR (2008) Use of the physiologically-based extraction test to assess the oral bioaccessibility of metals in vegetable plants grown in contaminated soil. Environ Pollut 152:60–72
Krishnamurtim GSR, Cieslinski G, Huang PM et al (1997) Kinetics of cadmium release from soils as influenced by organic acids: implication in cadmium availability. J Environ Qual 26:271–277
Lindsay WL, Norvell WA (1978) Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Sci Soc Am J 42:421–428
Lo IMC, Yang XY (1999) EDTA extraction of heavy metals from different soil fractions and synthetic soils. Water Air Soil Pollut 109:219–236
Luo X, Yu S, Li X (2011) Distribution, availability, and sources of trace metals in different particle size fractions of urban soils in Hong Kong: implications for assessing the risk to human health. Environ Pollut 159:1317–1326
Luo XS, Ding J, Xu B et al (2012a) Incorporating bioaccessibility into human health risk assessment of heavy metals in urban park soils. Sci Total Environ 424:88–96
Luo X, Yu S, Li X (2012b) The mobility, bioavailability, and human bioaccessibility of trace metals in urban soils of Hong Kong. Appl Geochem 27:995–1004
Madrid F, Diaz-Barrientos E, Madrid L (2008) Availability and bio-accessibility of metals in the clay fraction of urban soils of Sevilla. Environ Pollut 156:605–610
Mench M, Morel JL, Guckert A et al (1988) Metal binding with root exudates of low molecular weight. J Soil Sci 39:521–527
Mercier G, Duchesne J, Carles-Gibergues A (2002) A simple and fast screening test to detect soils polluted by lead. Environ Pollut 118:285–296
Nakos G (1987) Phosphorus adsorption by forest soils. Commun Soil Sci Plan 18:279–286
Oomen AG, Hack A, Minekus M et al (2002) Comparison of five in vitro digestion models to study the bioaccessibility of soil contaminants. Environ Sci Technol 36:3326–3334
Pelfrene A, Waterlot C, Mazzuca M et al (2011) Assessing Cd, Pb, Zn human bioaccessibility in smelter-contaminated agricultural topsoils (northern France). Environ Geochem Health 33:477–493
Poggio L, Vrscaj B, Schulin R et al (2009) Metals pollution and human bioaccessibility of topsoils in Grugliasco (Italy). Environ Pollut 157:680–689
Pueyo M, Lopez-Sanchez JF, Rauret G (2004) Assessment of CaCl2, NaNO3 and NH4NO3 extraction procedures for the study of Cd, Cu, Pb and Zn extractability in contaminated soils. Anal Chim Acta 504:217–226
Rao CRM, Sahuquillo A, Lopez Sanchez JF et al (2008) A review of the different methods applied in environment geochemistry for single and sequential extraction of trace elements in soils and related materials. Water Air Soil Pollut 189:291–333
Rauret G (1998) Extraction procedures for the determination of heavy metals in contaminated soil and sediment. Talanta 46:449–455
Roussel H, Waterlot C, Pelfrene A et al (2010) Cd, Pb and Zn oral bioaccessiblity of urban soils contaminated in the past by atmospheric emissions from two lead and zinc smelters. Arch Environ Contam Toxicol 58(4):945–954
Ruby MV, Davis A, Link TE et al (1993) Development of an in vitro screening test to evaluate the in vivo bioaccessibility of ingested mine-waste lead. Environ Sci Technol 27:2870–2877
Ruby MV, Davis A, Schoof R et al (1996) Estimation of lead and arsenic bioavailability using a physiologically based extraction test. Environ Sci Technol 30:422–430
Schroder JL, Basta NT, Casteel SW et al (2004) Validation of the in vitro gastrointestinal (IVG) method to estimate relative bioavailable lead in contaminated soils. J Environ Qual 33:513–521
SEPA, State Environmental Protection Agency (1995) Environmental quality standard for soils, China. GB15618–1995 (in Chinese)
SEPA, State Environmental Protection Agency (1997) Soil quality-determination of copper and zinc: flame atomic absorption spectrophotemetry, China. GB/T 17138–1997 (in Chinese)
Sialelli J, Urquhart GJ, Davidson CM et al (2010) Use of a physiologically based extraction test to estimate the human bioaccessibility of potentially toxic elements in urban soils from the city of Glasgow, UK. Environ Geochem Health 32:517–527
Sialelli J, Davidson CM, Hursthouse AS et al (2011) Human bioaccessibility of Cr, Cu, Ni, Pb and Zn in urban soils from the city of Torino, Italy. Environ Chem Lett 9:197–202
Takada A, Tsukada H, Takaku Y (2006) Extractability of major and trace elements from agricultural soils using chemical extraction methods: application for phytoavailability assessment. Soil Sci Plant Nutr 52:406–417
Tipping E, Rieuwerts J, Pan G et al (2003) The solid-solution partitioning of heavy metals (Cu, Zn, Cd, Pb) in upland soils of England and Wales. Environ Pollut 125:213–225
Turner A, Ip KH (2007) Bioaccessibility of metals in dust from the indoor environment: application of a physiologically based extraction test. Environ Sci Technol 41:7851–7856
Turner A, Radford A (2010) Bioaccessibility of trace metals in boat paint particles. Ecotoxicol Environ Saf 73:817–824
Wragg J, Cave M, Basta N et al (2011) An inter-laboratory trial of the unified BARGE bioaccessibility method for arsenic, cadmium and lead in soil. Sci Total Environ 409:4016–4030
Yeomans JC, Bremner JM (1988) A rapid and precise method for routine determination of organic carbon in soil. Comm Soil Sci Plant Anal 19:1467–1476
Zapusek U, Lestan D (2009) Fractionation, mobility and bioaccessibility of Cu, Zn, Cd, Pb and Ni in aged artificial soil mixtures. Geoderma 154(1–2):164–169
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This research was financially supported by the National Natural Science Foundation of China (Grant No. 21177108).
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Li, Y., Zhang, Mk. A comparison of physiologically based extraction test (PBET) and single-extraction methods for release of Cu, Zn, and Pb from mildly acidic and alkali soils. Environ Sci Pollut Res 20, 3140–3148 (2013). https://doi.org/10.1007/s11356-012-1234-0
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DOI: https://doi.org/10.1007/s11356-012-1234-0