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Development of environmental DNA chip for monitoring the invasive alien fishes in dam reservoirs

  • Special Feature: Original Paper
  • Environmental DNA as a Practical Tool for Aquatic Conservation and Restoration
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A Correction to this article was published on 16 September 2022

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Abstract

Control and prevention of invasive alien species (IAS) are important issues for the environmental management of dam reservoirs. Recently, environmental DNA (eDNA) analysis has been used for monitoring IAS in aquatic ecosystems. In this study, as a simple and user-friendly eDNA method for monitoring IAS, we developed an eDNA chip analysis for monitoring three IAS, largemouth bass Micropterus salmoides, smallmouth bass Micropterus dolomieu and bluegill sunfish Lepomis macrochirus, in dam reservoirs. The eDNA chip analysis using each IAS-specific primer and probe only amplified and detected the target species. The developed eDNA chip detected three IAS from water samples collected in five dam reservoirs, indicating that eDNA chip analysis can be used as a monitoring tool for IAS. However, IAS were not detected by the eDNA chip at some survey sites where the qPCR assays showed low eDNA concentrations, indicating a lower sensitivity of the eDNA chip at low eDNA concentrations. For the establishment of an eDNA chip for biomonitoring, some challenges faced by other techniques like qPCR and metabarcoding remain, such as sensitivity at low target concentrations and “real-time” detections in the field and further improvement of performance in eDNA chip will be required.

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Acknowledgements

We thank N. Kashiwadate, R. Nakatsugawa, M. Goto, Y. Maebara, Y. Kato, A. Shibahara, K. Kume, N. Yasuda, T. Sugiyama for supporting the field work and laboratory experiments. We also thank S. Miyazono for valuable help to check the manuscript. This work was supported by WEC Applied Ecological Study Fund (no. 2019-03).

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Authors and Affiliations

  1. Graduate School of Science and Technology for Innovation, Yamaguchi University, 2-16-1, Tokiwadai, Ube, Yamaguchi, 755-8611, Japan

    Ryohei Nakao & Yoshihisa Akamatsu

  2. Toyo Kohan Co., Ltd, 2-18-1, Higashi-Gotanda, Shinagawa-ku, Tokyo, Japan

    Ryo Miyata, Noriaki Nakamura, Marie Muramatsu & Hiroshi Okamura

  3. Research and Development Center, Nippon Koei Co., Ltd, 2304, Inarihara, Tsukuba, Ibaraki, Japan

    Fumiko Imamura

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  1. Ryohei Nakao
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  2. Ryo Miyata
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  3. Noriaki Nakamura
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  5. Hiroshi Okamura
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Correspondence to Ryohei Nakao.

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We have read the journal’s policy and the authors of this manuscript have the following competing interests: RM, NN, MM, HO is currently a paid employee of Toyo Kohan.

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The original online version of this article was revised as dam descriptions were replaced with Reservoir A, B, C, D, E in the body text, Figure 2, Table 2 and in supplementary file.

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Nakao, R., Miyata, R., Nakamura, N. et al. Development of environmental DNA chip for monitoring the invasive alien fishes in dam reservoirs. Landscape Ecol Eng 19, 33–41 (2023). https://doi.org/10.1007/s11355-022-00513-x

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  • DOI: https://doi.org/10.1007/s11355-022-00513-x

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