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Response to Deep Brain Stimulation in the Lateral Hypothalamic Area in a Rat Model of Obesity: In Vivo Assessment of Brain Glucose Metabolism

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An Erratum to this article was published on 09 February 2016

Abstract

Purpose

To investigate changes in glucose brain metabolism after deep brain stimulation (DBS) in the lateral hypothalamic area (LHA) in a rat model of obesity.

Procedures

Ten obese male Zucker rats were divided into two groups: LHA-control and LHA-DBS. Concentric bipolar platinum-iridium electrodes were implanted bilaterally. After 7 days, DBS was applied for 15 days. Weight and food and water intake were monitored. 2-Deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) PET-CT imaging studies were performed the day after the end of DBS. Differences in glucose uptake between the groups were assessed with statistical parametric mapping.

Results

A difference in weight gain of 3.19 percentage points was found between groups. Average food consumption during the first 15 days was lower in DBS-treated animals than in non-stimulated animals. DBS increased metabolism in the mammillary body, subiculum-hippocampal area, and amygdala, while a decrease in metabolism was recorded in the thalamus, caudate, temporal cortex, and cerebellum.

Conclusions

DBS produced significant changes in brain regions associated with the control of food intake and the brain reward system. DBS seems to normalize the impaired hippocampal functioning that has been described in obese rats. The smaller weight gain in the DBS group suggests that this technique could be considered an option for the treatment of obesity.

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Acknowledgements

We thank Alexandra de Francisco and Yolanda Sierra for their support in stereotactic surgery and animal handling. This research was supported by the following grants: Ministerio de Ciencia e Innovación (PI11/00616, PI10/02986, TEC2010-21619-C04-01) and Comunidad de Madrid (ARTEMIS S2009/DPI-1802). Support was also received from Fundación Mapfre.

Conflicts of Interest

None.

Authorship

MLSM designed the study, analyzed the data, and wrote the manuscript. JP and MD contributed to data analysis and discussion of the manuscript.

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Authors and Affiliations

  1. Unidad de Medicina y Cirugía Experimental, Instituto de Investigación Sanitaria Gregorio Marañón, Dr. Esquerdo, 46, Madrid, Spain

    María Luisa Soto-Montenegro & Manuel Desco

  2. CIBER de Salud Mental (CIBERSAM), Madrid, Spain

    María Luisa Soto-Montenegro, Javier Pascau & Manuel Desco

  3. Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, Madrid, Spain

    Javier Pascau & Manuel Desco

Authors
  1. María Luisa Soto-Montenegro
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Correspondence to María Luisa Soto-Montenegro.

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Soto-Montenegro, M.L., Pascau, J. & Desco, M. Response to Deep Brain Stimulation in the Lateral Hypothalamic Area in a Rat Model of Obesity: In Vivo Assessment of Brain Glucose Metabolism. Mol Imaging Biol 16, 830–837 (2014). https://doi.org/10.1007/s11307-014-0753-0

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  • DOI: https://doi.org/10.1007/s11307-014-0753-0

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