Abstract
Purpose
We investigated the early-stage fatty streaks/plaques detection using magnetomotive optical coherence tomography (MM-OCT) in conjunction with αvβ3 integrin-targeted magnetic microspheres (MSs). The targeting of functionalized MSs was investigated by perfusing ex vivo aortas from an atherosclerotic rabbit model in a custom-designed flow chamber at physiologically relevant pulsatile flow rates and pressures.
Procedures
Aortas were extracted and placed in a flow chamber. Magnetic MS contrast agents were perfused through the aortas and MM-OCT, fluorescence confocal, and bright field microscopy were performed on the ex vivo aorta specimens for localizing the MSs.
Results
The results showed a statistically significant and stronger MM-OCT signal (3.30 ± 1.73 dB) from the aorta segment perfused with targeted MSs, compared with the nontargeted MSs (1.18 ± 0.94 dB) and control (0.78 ± 0.41 dB) aortas. In addition, there was a good co-registration of MM-OCT signals with confocal microscopy.
Conclusions
Early-stage fatty streaks/plaques have been successfully detected using MM-OCT in conjunction with αvβ3 integrin-targeted magnetic MSs.
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Acknowledgments
This research was supported in part by grants from the National Institutes of Health (NIBIB R01 EB009073) and a sponsored research agreement with Samsung, Inc. Jongsik Kim was funded by a Carle Foundation Hospital-Beckman Institute fellowship. Adeel Ahmad was funded at the University of Illinois by the NIH National Cancer Institute Alliance for Nanotechnology in Cancer (Midwest Cancer Nanotechnology Training Center) Grant R25-CA154015A.
Disclosures
All other authors declare that they have no conflict of interest except for Stephen A. Boppart who receives royalties from the Massachusetts Institute of Technology for patents related to optical coherence tomography.
Conference presentation
Ahmad A, Kim JS, Li J, et al. Magnetomotive contrast in optical coherence tomography for detecting early-stage atherosclerosis using targeted microspheres, Optical Society of America, Biomedical Optics (BIOMED), Miami, Florida, 29 April–2 May 2012.
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Jongsik Kim and Adeel Ahmad both contributed equally to this work.
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Kim, J., Ahmad, A., Marjanovic, M. et al. Magnetomotive Optical Coherence Tomography for the Assessment of Atherosclerotic Lesions Using αvβ3 Integrin-Targeted Microspheres. Mol Imaging Biol 16, 36–43 (2014). https://doi.org/10.1007/s11307-013-0671-6
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DOI: https://doi.org/10.1007/s11307-013-0671-6