Skip to main content
SpringerLink
Log in

MR Detection of LPS-Induced Neutrophil Activation using Mannan-Coated Superparamagnetic Iron Oxide Nanoparticles

  • Research Article
  • Published:
Molecular Imaging and Biology Aims and scope Submit manuscript

Abstract

Purpose

The purpose of this study was to investigate the potential of the phagocytic activity of lipopolysaccharide (LPS) induced neutrophils that are mannan coated with superparamagnetic iron oxide nanoparticles (M-SPION).

Procedures

Human neutrophils were divided into control and LPS groups. The neutrophils were labeled with M-SPION and dextran-coated SPION. After labeling of M-SPION, the mean signal intensity (SI) of the LPS group was significantly lower than that of the control group.

Results

The labeling of both control and LPS groups with M-SPION showed significantly lower SI than those labeled with D-SPION. After labeling with M-SPION, the intracellular iron uptake of neutrophil in Prussian blue staining was markedly demonstrated in the LPS group, but not in the control group. M-SPION was more effective than D-SPION in the labeling of neutrophils in vitro.

Conclusions

The in vitro labeling technique of LPS neutrophil with M-SPION on MR imaging could be developed into a diagnostic method of LPS-induced neutrophils.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Bone RC, Balk RA, Cerra FB et al (1992) Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine. Chest 101:1644–1655

    Article  PubMed  CAS  Google Scholar 

  2. Kibe S, Adams K, Barlow G (2011) Diagnostic and prognostic biomarkers of sepsis in critical care. J Antimicrob Chemother 66(Suppl 2):ii33–ii40

    Article  PubMed  CAS  Google Scholar 

  3. Wheeler AP, Bernard GR (1999) Treating patients with severe sepsis. N Engl J Med 340:207–214

    Article  PubMed  CAS  Google Scholar 

  4. Meisner M, Tschaikowsky K, Palmaers T, Schmidt J (1999) Comparison of procalcitonin (PCT) and C-reactive protein (CRP) plasma concentrations at different SOFA scores during the course of sepsis and MODS. Crit Care 3:45–50

    Article  PubMed  Google Scholar 

  5. Weissleder R, Elizondo G, Wittenberg J, Rabito CA, Bengele HH, Josephson L (1990) Ultrasmall superparamagnetic iron oxide: characterization of a new class of contrast agents for MR imaging. Radiology 175:489–493

    PubMed  CAS  Google Scholar 

  6. Daldrup-Link HE, Rudelius M, Oostendorp RA et al (2003) Targeting of hematopoietic progenitor cells with MR contrast agents. Radiology 228:760–767

    Article  PubMed  Google Scholar 

  7. Fleige G, Seeberger F, Laux D et al (2002) In vitro characterization of two different ultrasmall iron oxide particles for magnetic resonance cell tracking. Investig Radiol 37:482–488

    Article  CAS  Google Scholar 

  8. Zelivyanskaya ML, Nelson JA, Poluektova L et al (2003) Tracking superparamagnetic iron oxide labeled monocytes in brain by high-field magnetic resonance imaging. J Neurosci Res 73:284–295

    Article  PubMed  CAS  Google Scholar 

  9. Sipe JC, Filippi M, Martino G et al (1999) Method for intracellular magnetic labeling of human mononuclear cells using approved iron contrast agents. Magn Reson Imaging 17:1521–1523

    Article  PubMed  CAS  Google Scholar 

  10. Metz S, Bonaterra G, Rudelius M, Settles M, Rummeny EJ, Daldrup-Link HE (2004) Capacity of human monocytes to phagocytose approved iron oxide MR contrast agents in vitro. Eur Radiol 14:1851–1858

    Article  PubMed  Google Scholar 

  11. Alves-Filho JC, de Freitas A, Spiller F, Souto FO, Cunha FQ (2008) The role of neutrophils in severe sepsis. Shock 30(Suppl 1):3–9

    Article  PubMed  CAS  Google Scholar 

  12. Stein M, Keshav S, Harris N, Gordon S (1992) Interleukin 4 potently enhances murine macrophage mannose receptor activity: a marker of alternative immunologic macrophage activation. J Exp Med 176:287–292

    Article  PubMed  CAS  Google Scholar 

  13. Wong R, Shou J, Wang Y (2010) Probing sepsis and sepsis-like conditions using untargeted SPIO nanoparticles. Conf Proc 2010:3053–3056, Annual International Conference of the IEEE Engineering in Medicine and Biology Society IEEE Engineering in Medicine and Biology Society Conference

    Google Scholar 

  14. Yoo MK, Park IY, Kim IY et al (2008) Superparamagnetic iron oxide nanoparticles coated with mannan for macrophage targeting. J Nanosci Nanotechnol 8:5196–5202

    Article  PubMed  CAS  Google Scholar 

  15. Vu-Quang H, Yoo MK, Jeong HJ et al (2011) Targeted delivery of mannan-coated superparamagnetic iron oxide nanoparticles to antigen-presenting cells for magnetic resonance-based diagnosis of metastatic lymph nodes in vivo. Acta Biomater 7:3935–3945

    Article  PubMed  CAS  Google Scholar 

  16. Abraham E, Gyetko MR, Kuhn K et al (2003) Urokinase-type plasminogen activator potentiates lipopolysaccharide-induced neutrophil activation. J Immunol 170:5644–5651

    PubMed  CAS  Google Scholar 

  17. Schnaitman CA, Klena JD (1993) Genetics of lipopolysaccharide biosynthesis in enteric bacteria. Microbiol Rev 57:655–682

    PubMed  CAS  Google Scholar 

  18. Suffredini AF, Fromm RE, Parker MM et al (1989) The cardiovascular response of normal humans to the administration of endotoxin. N Engl J Med 321:280–287

    Article  PubMed  CAS  Google Scholar 

  19. Martich GD, Danner RL, Ceska M, Suffredini AF (1991) Detection of interleukin 8 and tumor necrosis factor in normal humans after intravenous endotoxin: the effect of antiinflammatory agents. J Exp Med 173:1021–1024

    Article  PubMed  CAS  Google Scholar 

  20. Jung CW, Jacobs P (1995) Physical and chemical properties of superparamagnetic iron oxide MR contrast agents: ferumoxides, ferumoxtran, ferumoxsil. Magn Reson Imaging 13:661–674

    Article  PubMed  CAS  Google Scholar 

  21. Dousset V, Delalande C, Ballarino L et al (1999) In vivo macrophage activity imaging in the central nervous system detected by magnetic resonance. Magn Reson Med 41:329–333, Official Journal of the Society of Magnetic Resonance in Medicine/Society of Magnetic Resonance in Medicine

    Article  PubMed  CAS  Google Scholar 

  22. Floris S, Blezer EL, Schreibelt G et al (2004) Blood–brain barrier permeability and monocyte infiltration in experimental allergic encephalomyelitis: a quantitative MRI study. Brain 127:616–627

    Article  PubMed  CAS  Google Scholar 

  23. Krieg FM, Andres RY, Winterhalter KH (1995) Superparamagnetically labelled neutrophils as potential abscess-specific contrast agent for MRI. Magn Reson Imaging 13:393–400

    Article  PubMed  CAS  Google Scholar 

  24. Matuszewski L, Persigehl T, Wall A et al (2005) Cell tagging with clinically approved iron oxides: feasibility and effect of lipofection, particle size, and surface coating on labeling efficiency. Radiology 235:155–161

    Article  PubMed  Google Scholar 

  25. Burgdorf S, Lukacs-Kornek V, Kurts C (2006) The mannose receptor mediates uptake of soluble but not of cell-associated antigen for cross-presentation. J Immunol 176:6770–6776

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (MEST) (2011–0030034) and supported by a grant (CRI 12051–22) Chonnam National University Hospital Biomedical Research Institute.

Conflict of Interest

The authors have no conflicts of interest regarding this study publication.

Author information

Authors and Affiliations

  1. Department of Radiology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju, 501-757, South Korea

    Han Shanhua, Myeong Ju Moon, Suk Hee Heo, Hyo Soon Lim & Yong Yeon Jeong

  2. Department of Anesthesiolgy, Chonnam National University Hospital, Chonnam National University Medical School, Gwangju, 501-757, South Korea

    Han Huijing & Sang Hyun Kwak

  3. Department of Biomedical Sciences and Center for Biomedical Human Resources (BK-21 project), Chonnam National University Medical School, Gwangju, 501-757, South Korea

    In-Kyu Park

  4. Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, South Korea

    Chong-Su Cho

Authors
  1. Han Shanhua
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Han Huijing
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Myeong Ju Moon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Suk Hee Heo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hyo Soon Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. In-Kyu Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Chong-Su Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Sang Hyun Kwak
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yong Yeon Jeong
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yong Yeon Jeong.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shanhua, H., Huijing, H., Moon, M.J. et al. MR Detection of LPS-Induced Neutrophil Activation using Mannan-Coated Superparamagnetic Iron Oxide Nanoparticles. Mol Imaging Biol 15, 685–692 (2013). https://doi.org/10.1007/s11307-013-0643-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11307-013-0643-x

Key words

Search

Navigation