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Molecular Imaging of Cell Death in an Experimental Model of Parkinson’s Disease with a Novel Apoptosis-Targeting Peptide

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Abstract

Purpose

We used a novel apoptosis-targeting peptide called ApoPep-1 in order to evaluate whether ApoPep-1 can be used as a diagnostic indicator in a model of Parkinson’s disease (PD).

Procedures

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was given to mice to produce the PD model. Cy7.5-labeled ApoPep-1 was given intravenously and optical imaging was taken at 1, 2, and 3 weeks after MPTP treatment. Immunohistochemical study was performed with brain sections.

Results

Increased ApoPep-1 signal was observed in the brain of MPTP-treated mice by in vivo and ex vivo imaging study. With histological evaluation, ApoPep-1 signal demonstrated a strong correlation with loss of dopaminergic neurons or increase of apoptotic cells. Moreover, the neuroprotective effect of amantadine in the MPTP model was effectively evaluated using optical imaging of ApoPep-1.

Conclusions

We conclude that ApoPep-1 is the effective probe for imaging of apoptosis in the MPTP model and can be applied in brain diseases with apoptosis.

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Abbreviations

PD:

Parkinson’s disease

SNpc:

Substantia nigra parts compacta

MPTP:

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine

TUNEL:

Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay

DNA:

Deoxyribonucleic acid

PBS:

Phosphate-buffered saline

BSA:

Bovine serum albumin

TH:

Tyrosine hydroxylase

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Acknowledgment

This work was supported by grant no. RTI04-01-01 from the Regional Technology Innovation Program of the Ministry of Knowledge Economy (MKE) & Converging Research Center Program through the Ministry of Education, Science and Technology (2010K001054).

Conflict of Interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Physiology, Kyungpook National University School of Medicine, 101 Dongin Dong 2 Ga, Jung Gu, Daegu, 700-422, Republic of Korea

    Min-Jeong Lee & Hyung Soo Han

  2. Department of Biochemistry and Cell Biology, Kyungpook National University School of Medicine, 101 Dongin Dong 2 Ga, Jung Gu, Daegu, 700-422, Republic of Korea

    Kai Wang, In-San Kim & Byung-Heon Lee

  3. Cell & Matrix Research Institute, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea

    Kai Wang, In-San Kim & Byung-Heon Lee

  4. Brain Science & Engineering Institute, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea

    Min-Jeong Lee & Hyung Soo Han

  5. Department of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea

    Kai Wang

Authors
  1. Min-Jeong Lee
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  2. Kai Wang
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Corresponding authors

Correspondence to Byung-Heon Lee or Hyung Soo Han.

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Lee, MJ., Wang, K., Kim, IS. et al. Molecular Imaging of Cell Death in an Experimental Model of Parkinson’s Disease with a Novel Apoptosis-Targeting Peptide. Mol Imaging Biol 14, 147–155 (2012). https://doi.org/10.1007/s11307-011-0497-z

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  • DOI: https://doi.org/10.1007/s11307-011-0497-z

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