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Clinical applications of breast cancer metabolomics using high-resolution magic angle spinning proton magnetic resonance spectroscopy (HRMAS 1H MRS): systematic scoping review

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Abstract

Introduction

Breast cancer is a heterogeneous disease with different prognoses and responses to systemic treatment depending on its molecular characteristics, which makes it imperative to develop new biomarkers for an individualized diagnosis and personalized oncological treatment. Ex vivo high-resolution magic angle spinning proton magnetic resonance spectroscopy (HRMAS 1H MRS) is the most common technique for metabolic quantification in human surgical and biopsy tissue specimens.

Objective

To perform a review of the current available literature on the clinical applications of HRMAS 1H MRS metabolic analysis in tissue samples of breast cancer patients.

Methods

This systematic scoping review included original research papers published in the English language in peer-reviewed journals. Study selection was performed independently by two reviewers and preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines were followed.

Results

The literature search returned 159 studies and 26 papers were included as part of this systematic review. There was considerable variation regarding tissue type, aims, and statistical analysis methods across the different studies. To facilitate the interpretation of the results, the included studies were grouped according to their aims or main outcomes into: feasibility and tumor diagnosis (n = 6); tumor heterogeneity (n = 2); correlation with proteomics/transcriptomics (n = 3); correlation with prognostic factors (n = 11); and response evaluation to NAC (n = 4).

Conclusion

There is a lot of potential in including metabolic information of breast cancer tissue obtained with HRMAS 1H MRS. To date, studies show that metabolic concentrations quantified by this technique can be related to the diagnosis, prognosis, and treatment response in breast cancer patients.

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Funding

This work was partially supported by the NIH/NCI Cancer Center Support Grant (P30 CA008748) and the Breast Cancer Research Foundation

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Authors and Affiliations

  1. Breast Imaging Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY, USA

    Almir G. V. Bitencourt, Katja Pinker & Sunitha B. Thakur

  2. Department of Imaging, A.C.Camargo Cancer Center, São Paulo, SP, Brazil

    Almir G. V. Bitencourt

  3. MSK Library, Memorial Sloan Kettering Cancer Center, New York, NY, USA

    Johanna Goldberg

  4. Department of Medical Physics, Memorial Sloan Kettering Cancer Center, 300 E 66th St, New York, NY, 10065, USA

    Sunitha B. Thakur

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  1. Almir G. V. Bitencourt
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  2. Johanna Goldberg
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Contributions

JG conducted the literature review and constructed the article database. AGVB and SBT performed the study selection and reviewed the selected studies. All authors were involved in writing the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Sunitha B. Thakur.

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Conflicts of interest

Katja Pinker received payment for activities not related to the present article including lectures including service on speakers bureaus and for travel/accommodations/meeting expenses unrelated to activities listed from the European Society of Breast Imaging (MRI educational course, annual scientific meeting) and the IDKD 2019 (educational course).

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Bitencourt, A.G.V., Goldberg, J., Pinker, K. et al. Clinical applications of breast cancer metabolomics using high-resolution magic angle spinning proton magnetic resonance spectroscopy (HRMAS 1H MRS): systematic scoping review. Metabolomics 15, 148 (2019). https://doi.org/10.1007/s11306-019-1611-5

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