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Integrative metabolomic analysis reveals diet supplementation with green tea alleviates UVB-damaged mouse skin correlated with ascorbate metabolism and urea cycle

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Abstract

Introduction

While green tea is known to protect skin from ultraviolet (UV) light, underlying damage-repair mechanisms remain unclear.

Objectives

The major objective of this study was to investigate, using multi-omics analysis, the metabolic mechanisms associated with the effects of a diet supplemented with green tea (SGT) in UVB-damaged mice.

Methods

Six to eight weeks old female Skh:HR-1 mice were randomly divided into three experimental groups i.e., non-irradiated with control diet (NOR), UVB-irradiated with control diet (UND), and UVB-irradiated with SGT (UGD), and subjected to experimental conditions over 10 weeks. The skin samples were analyzed by metabolomics, transcriptomics, and in silico modeling.

Results

Our results revealed that SGT significantly alleviated UVB-induced metabolite alterations (aspartic acid, ornithine, ascorbic acid, ethanolamine, and C20:0-lysophosphatidylethanolamine) and gene expressions (keratin sulfate biosynthesis/degradation, fatty acid oxidation, and steroid metabolism) in the skin. Among these changes, key metabolic pathways, including ascorbate metabolism and the urea cycle, were the major pathways mitigated through SGT diet among UGD group mice. Additionally, SGT treatment also affected serum and hepatic lysophospholipid levels through attenuating and intensifying UVB-induced metabolic changes, respectively.

Conclusion

Our results suggested that the SGT diets primarily influenced ascorbate metabolism and the urea cycle in UVB-irradiated mouse skin, alleviating deleterious UVB-induced skin wrinkles, epidermal thickening, and collagen-fiber destruction.

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Acknowledgements

This study was supported by the Bio & Medical Technology Development Program of the National Research Foundation funded by the Korean government, MSIP (2016M3A9A5923160); and the Korea Food Research Institute (E0164503-01).

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea

    Eun Sung Jung, Hye Min Park & Choong Hwan Lee

  2. Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin, 17104, Republic of Korea

    Seung Min Hyun & Jae Sung Hwang

  3. College of Pharmacy and Research Institute of Pharmaceutical Science, Kyungpook National University, Daegu, 41566, Republic of Korea

    Jong Cheol Shon & Kwang-Hyeon Liu

  4. Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01 Centros, Singapore, 138668, Singapore

    Meiyappan Lakshmanan, Hock Chuan Yeo & Dong-Yup Lee

  5. Natural Product Research Institute, Seoul National University, Seoul, 08826, Republic of Korea

    Minsoo Noh

  6. Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585, Singapore

    Hock Chuan Yeo & Dong-Yup Lee

  7. NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, 28 Medical Drive, Singapore, 117456, Singapore

    Dong-Yup Lee

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  1. Eun Sung Jung
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Correspondence to Jae Sung Hwang or Choong Hwan Lee.

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Jung, E.S., Park, H.M., Hyun, S.M. et al. Integrative metabolomic analysis reveals diet supplementation with green tea alleviates UVB-damaged mouse skin correlated with ascorbate metabolism and urea cycle. Metabolomics 13, 82 (2017). https://doi.org/10.1007/s11306-017-1218-7

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