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A metabolomics approach shows that catechin-enriched green tea attenuates ultraviolet B-induced skin metabolite alterations in mice

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Abstract

In this study, catechin-enriched green tea (CGT) administration significantly attenuated ultraviolet (UV) B-induced mouse skin alterations, i.e., increases in wrinkle formation, thickness, erythema, and transepidermal water loss, and decreases in hydration, elasticity, and collagen fiber content. The metabolites in the mouse skin after UVB irradiation and CGT administration were profiled using comprehensive MS-based metabolomics techniques such as ultra-performance liquid chromatography-quadrupole time-of-flight-mass spectrometry (TOF-MS), gas chromatography-TOF-MS, and NanoMate tandem-MS. From these analysis, we revel that Normal, UVB-irradiated, and UVB-irradiated with CGT administrated groups clearly discriminated from each other in partial least squares-discriminant analysis models, and CGT administration attenuated UVB-induced alteration of skin metabolites such as lysophospholipids, fatty acids, ceramides, amino acids, organic compounds, lipids, and nucleobases. Among them, purine nucleobases (inosine and hypoxanthine), ascorbic acid, and lactose were remarkably influenced by CGT administration, which indicated that these metabolites could be biomarkers to explain CGT effects on UVB-irradiated skin. Our results suggested that CGT administration was effective in reducing the levels of UVB-induced alterations of numerous mouse skin metabolites, and these metabolites were might highly relevant to observed changes in skin conditions.

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Acknowledgments

This study was supported by a grant of the Korea Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (Grant No.: HN13C0076) and of the Cooperative Research Program for Agricultural Science & Technology Development, Rural Department Administration, Republic of Korea. (No.: PJ 009826).

Conflict of interest

The all authors declared that they have no conflict of interest in the submission of this manuscript.

Compliance with Ethics Requirements

All procedures performed in studies involving animals were in accordance with ethical standards of the institution or practice at which the studies were conducted.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 143-701, Republic of Korea

    Eun Sung Jung, Hye Min Park & Choong Hwan Lee

  2. Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749, Republic of Korea

    Kyung-Eun Lee, Sukyeong Mun & Jae-Kwan Hwang

  3. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Jung-Hoon Shin & Kwang-Hyeon Liu

  4. Department of Biomaterials Science and Engineering, Yonsei University, Seoul, 120-749, Republic of Korea

    Jae-Kwan Hwang

  5. Food Research Institute, AmorePacific R&D Center, Yongin, 446-729, Republic of Korea

    Jeong Kee Kim & Sang Jun Lee

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  1. Eun Sung Jung
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  2. Hye Min Park
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Correspondence to Jae-Kwan Hwang or Choong Hwan Lee.

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Jung, E.S., Park, H.M., Lee, KE. et al. A metabolomics approach shows that catechin-enriched green tea attenuates ultraviolet B-induced skin metabolite alterations in mice. Metabolomics 11, 861–871 (2015). https://doi.org/10.1007/s11306-014-0743-x

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  • DOI: https://doi.org/10.1007/s11306-014-0743-x

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