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Adenosine enhances progenitor cell recruitment and nerve growth via its A2B receptor during adult fin regeneration

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Abstract

A major issue in regenerative medicine is the control of progenitor cell mobilisation. Apoptosis has been reported as playing a role in cell plasticity, and it has been recently shown that apoptosis is necessary for organ and appendage regeneration. In this context, we explore its possible mode of action in progenitor cell recruitment during adult regeneration in zebrafish. Here, we show that apoptosis inhibition impairs blastema formation and nerve growth, both of which can be restored by exogenous adenosine acting through its A2B receptor. Moreover, adenosine increases the number of progenitor cells. Purinergic signalling is therefore an early and essential event in the pathway from lesion to blastema formation and provides new targets for manipulating cell plasticity in the adult.

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Acknowledgments

The authors thank Alain Prochiantz for the helpful discussions and constant support.

Author information

Authors and Affiliations

  1. Centre Interdisciplinaire de Recherche en biologie (CIRB), CNRS UMR 7241//INSERM U1050, Collège de France, 11, Place Marcelin Berthelot, 75231, Paris Cedex 05, France

    Christine Rampon, Carole Gauron, Francesca Meda, Michel Volovitch & Sophie Vriz

  2. Biology Department, Université Paris Diderot, Sorbonne Paris Cité, 75205, Paris Cedex 13, France

    Christine Rampon & Sophie Vriz

  3. École Normale Supérieure, Institute of Biology at the Ecole Normale Supérieure (IBENS), CNRS UMR8197, INSERM U1024, PSL Research University, 75005, Paris, France

    Francesca Meda & Michel Volovitch

Authors
  1. Christine Rampon
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  2. Carole Gauron
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  3. Francesca Meda
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  4. Michel Volovitch
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  5. Sophie Vriz
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Corresponding author

Correspondence to Sophie Vriz.

Additional information

Christine Rampon and Carole Gauron contributed equally to this work.

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Fig. S1

Effect of AMP, ATP and adenosine receptor agonists and an antagonist on fin regeneration. Fish were incubated in drugs (nM) from the time of amputation to 3 dpa, and then the efficiency of regeneration was quantified and expressed as a percentage of the control. Representative fins are shown. Dotted lines indicate the amputation plane. Error bars represent the SEM (* p < 0.05, **p < 0.01, ***p < 0.001). n values were indicated on the bottom of each bar of the graph (JPEG 293 kb)

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Rampon, C., Gauron, C., Meda, F. et al. Adenosine enhances progenitor cell recruitment and nerve growth via its A2B receptor during adult fin regeneration. Purinergic Signalling 10, 595–602 (2014). https://doi.org/10.1007/s11302-014-9420-9

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