Abstract
P2X1 receptors, the major subtype of P2X receptors in the vascular smooth muscle, are essential for α,β-methylene adenosine 5′-triphosphate (α,β-MeATP)-induced vasoconstriction. However, relative physiological significance of P2X1 receptor-regulated vasoconstriction in the different types of arteries in the rat is not clear as compared with α1-adrenoceptor-regulated vasoconstriction. In the present study, we found that vasoconstrictive responses to noncumulative administration of α,β-MeATP in the rat isolated mesenteric arteries were significantly smaller than those to single concentration administration of α,β-MeATP. Therefore, we firstly reported the characteristic of α,β-MeATP-regulated vasoconstrictions in rat tail, internal carotid, pulmonary, mesenteric arteries, and aorta using single concentration administration of α,β-MeATP. The rank order of maximal vasoconstrictions for α,β-MeATP (E max·α,β-MeATP) was the same as that of maximal vasoconstrictions for noradrenaline (E max·NA) in the internal carotid, pulmonary, mesenteric arteries, and aorta. Moreover, the value of (E max·α,β-MeATP/E max·KCl)/(E max·NA/E max·KCl) was 0.4 in each of the four arteries, but it was 0.8 in the tail artery. In conclusion, P2X1 receptor-mediated vasoconstrictions are equally important in rat internal carotid, pulmonary, mesenteric arteries, and aorta, but much greater in the tail artery, suggesting its special role in physiological function.
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Kennedy C, Saville VL, Burnstock G (1986) The contributions of noradrenaline and ATP to the response of the rabbit central ear artery to sympathetic nerve stimulation depend on the parameters of stimulation. Eur J Pharmacol 122:291–300
Burnstock G, Warland JJ (1987) A pharmacological study of the rabbit saphenous artery in vitro: a vessel with a large purinergic contractile response to sympathetic nerve stimulation. Br J Pharmacol 90:111–120
Brizzolara AL, Burnstock G (1990) Evidence for noradrenergic–purinergic cotransmission in the hepatic artery of the rabbit. Br J Pharmacol 99:835–839
Ren LM, Burnstock G (1997) Prominent sympathetic purinergic vasoconstriction in the rabbit splenic artery: potentiation by 2,2′-pyridylisatogen tosylate. Br J Pharmacol 120:530–536
Bo X, Burnstock G (1993) Heterogeneous distribution of [3H]α, β-methylene ATP binding sites in blood vessels. J Vasc Res 30:87–101
Ramme D, Regenold JT, Starke K, Busse R, Illes P (1987) Identification of the neuroeffector transmitter in jejunal branches of rabbit mesenteric artery. Naunyn–Schmiedeberg’s Arch Pharmacol 336:267–273
Boarder MR, Hourani SM (1998) The regulation of vascular function by P2 receptors: multiple sites and multiple receptors. Trends Pharmacol Sci 19:99–107
Valera S, Hussy N, Evans RJ, Adami N, North RA, Surprenant A, Buell G (1994) A new class of ligand-gated ion channel defined by P2X receptor for extracellular ATP. Nature 371:516–519
Collo G, North RA, Kawashima E, Merlo-Pich E, Neidhart S, Surprenant A, Buell G (1996) Cloning of P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels. J Neurosci 16:2495–2507
Galligan JJ, Hess MC, Miller SB, Fink GD (2001) Differential localization of P2 receptor subtypes in mesenteric arteries and veins of normotensive and hypertensive rats. J Pharmacol Exp Ther 296:478–485
Vial C, Evans RJ (2002) P2X1 receptor-deficient mice establish the native P2X receptor and a P2Y6-like receptor in arteries. Mol Pharmacol 62:1438–1445
Erlinge D, Burnstock G (2008) P2 receptors in cardiovascular regulation and disease. Purinergic Signal 4:1–20
Steinmetz M, Bierer S, Hollah P, Rahn KH, Schlatter E (2000) Heterogenous vascular effects of AP5A in different rat resistance arteries are due to heterogenous distribution of P2X and P2Y1 purinoceptors. J Pharmacol Exp Ther 294:1182–1187
Malmsjö M, Bergdahl A, Möller S, Zhao XH, Sun XY, Hedner T, Edvinsson L, Erlinge D (1999) Congestive heart failure induces downregulation of P2X1-receptors in resistance arteries. Cardiovasc Res 43:219–227
Wihlborg AK, Slätt J, Sun X, Zhao XH, Malmsjö M, Bergman J, Hedner T, Erlinge D (2003) 2,2′-Nitrophenylisatogen potentiates P2X1 receptor mediated vascular contraction and blood pressure elevation. Drug Dev Res 59:82–87
Liu SF, McCormack DG, Evans TW, Barnes PJ (1989) Characterization and distribution of P2-purinoceptor subtypes in rat pulmonary vessels. J Pharmacol Exp Ther 251:1204–1210
Mombouli JV, Vanhoutte PM (1993) Purinergic endothelium-dependent and -independent contractions in rat aorta. Hypertension 22:577–583
O’Connor SE, Wood BE, Leff P (1990) Characterization of P2x -receptors in rabbit isolated ear artery. Br J Pharmacol 101:640–644
Zhao D, Ren LM (2005) Non-adrenergic inhibition at prejunctional sites by agmatine of purinergic vasoconstriction in rabbit saphenous artery. Neuropharmacology 48:597–606
Zhao D, Ren LM, Lu HG, Zhang X (2008) Potentiation by yohimbine of α-adrenoceptor-mediated vasoconstriction in response to clonidine in the rabbit ear vein. Eur J Pharmacol 589:201–205
Park JY, Shin HK, Lee YJ, Choi YW, Bae SS, Kim CD (2009) The mechanism of vasorelaxation induced by Schisandra chinensis extract in rat thoracic aorta. J Ethnopharmacol 121:69–73
Oriowo MA, Chandrasekhar B, Kadavil EA (2003) α1-Adrenoceptor subtypes mediating noradrenaline-induced contraction of pulmonary artery from pulmonary hypertensive rats. Eur J Pharmacol 482:255–263
Massett MP, Lewis SJ, Bates JN, Kregel KC (1998) Effect of heating on vascular reactivity in rat mesenteric arteries. J Appl Physiol 85:701–708
Jähnichen S, Eltze M, Pertz HH (2004) Evidence that α1B-adrenoceptors are involved in noradrenaline-induced contractions of rat tail artery. Eur J Pharmacol 488:157–167
Leff P, Wood BE, O’Connor SE (1990) Suramin is a slowly-equilibrating but competitive antagonist at P2X-receptors in the rabbit isolated ear artery. Br J Pharmacol 101:645–649
Burnstock G, Kennedy C (1985) Is there a basis for distinguishing two types of P2-purinoceptor? Gen Pharmacol 16:433–440
Ren LM, Zhang M (2002) Distribution of functional P2X1-like receptor in isolated rabbit arteries. Acta Pharmacol Sin 23:721–726
Muramatsu I, Ohmura T, Kigoshi S, Hashimoto S, Oshita M (1990) Pharmacological subclassification of α-adrenoceptors in vascular smooth muscle. Br J Pharmacol 99:197–201
Chootip K, Ness KF, Wang Y, Gurney AM, Kennedy C (2002) Regional variation in P2 receptor expression in the rat pulmonary arterial circulation. Br J Pharmacol 137:637–646
Vails AJ, Crowe R, Burnstock G (1997) A neuromodulatory role for neuronal nitric oxide in the rabbit renal artery. Br J Pharmacol 121:213–220
Bo X, Sexton A, Xiang Z, Nori SL, Burnstock G (1998) Pharmacological and histochemical evidence for P2X receptors in human umbilical vessels. Eur J Pharmacol 353:59–65
Garcia-Villalon AL, Garcia JL, Fernandez N, Monge L, Gomez B, Dieguez G (1996) Regional differences in the arterial response to vasopressin: role of endothelial nitric oxide. Br J Pharmacol 118:1848–1854
Nori S, Fumagalli L, Bo X, Bogdanov Y, Burnstock G (1998) Coexpression of mRNAs for P2X1, P2X2 and P2X4 receptors in rat vascular smooth muscle: an in situ hybridization and RT-PCR study. J Vasc Res 35:179–185
Ralevic V, Burnstock G (1998) Receptors for purines and pyrimidines. Pharmacol Rev 50:413–492
Hansen MA, Dutton JL, Balcar VJ, Barden JA, Bennett MR (1999) P2X (purinergic) receptor distributions in rat blood vessels. J Auton Nerv Syst 75:147–155
Brake AJ, Wagenbach MJ, Julius D (1994) New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor. Nature 371:519–523
Wallace A, Knight GE, Cowen T, Burnstock G (2006) Changes in purinergic signalling in developing and ageing rat tail artery: importance for temperature control. Neuropharmacology 50:191–208
Rand RP, Burton AC, Ing T (1965) The tail of the rat, in temperature regulation and acclimatization. Can J Physiol Pharmacol 43:257–267
Raman ER, Vanhuyse VJ, Roberts MF (1987) Mathematical circulation model for the blood-flow–heat-loss relationship in the rat tail. Phys Med Biol 32:859–875
Ajay M, Achike FI, Mustafa MR (2007) Modulation of vascular reactivity in normal, hypertensive and diabetic rat aortae by a non-antioxidant flavonoid. Pharmacol Res 55:385–391
Yu J, Tokinaga Y, Kuriyama T, Uematsu N, Mizumoto K, Hatano Y (2005) Involvement of Ca2+ sensitization in ropivacaine-induced contraction of rat aortic smooth muscle. Anesthesiology 103:548–555
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This work was supported by a grant from the National Program on Key Basic Research Project of China (973 Program; No. 2005CB523301).
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Li, L., Jia, ZH., Chen, C. et al. Physiological significance of P2X receptor-mediated vasoconstriction in five different types of arteries in rats. Purinergic Signalling 7, 221–229 (2011). https://doi.org/10.1007/s11302-011-9226-y
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DOI: https://doi.org/10.1007/s11302-011-9226-y