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Development of EST-SSR markers and investigation of genetic relatedness in tung tree

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Abstract

The tung tree is an important non-edible oilseed source and consists of two species Vernicia fordii and Vernicia montana native to southern China and southeast Asia. In this study, the frequency and distribution of simple sequence repeats (SSRs) in expressed sequence tags (ESTs) of V. fordii were characterized based on 2,407 available EST sequences from the National Center for Biotechnology Information database. Twenty-two EST-SSR markers were developed by screening the genomic DNAs of six individuals of V. fordii from three accessions and 120 individuals of V. montana from 30 indigenous V. montana accessions collected from different geographic areas in southwestern China and northern Laos. The 22 EST-SSR markers exhibited a moderate level of polymorphism in V. montana with an average of 3.36 alleles per locus and PIC of 0.401. Genetic relatedness investigation showed that there was not only distinct genetic differentiation among tung trees but also a distinct geographic pattern among V. montana accessions. The current study is the first report of the development of EST-SSRs and genetic relatedness investigation in tung trees. These EST-SSR markers reported here will be valuable resources for future genetic studies, like construction of linkage maps, diversity analysis, quantitative trait locus/association mapping, and molecular breeding of the tung tree. The genetic relatedness identified in V. montana would provide potential clues in choosing germplasms in interest as progenitors for cross breeding and variety improvement of V. montana in practice.

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Acknowledgments

This research was jointly supported by the “100 Talents” Program of the Chinese Academy of Sciences (to AL) and research programs of the Yunnan Natural Science and Technology (2009FXS0021).

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Authors and Affiliations

  1. Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 88 Xuefu Road, Kunming, 650223, Yunnan, China

    Wei Xu, Qing Yang & Aizhong Liu

  2. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, Jiangsu, China

    Wei Xu & Huyin Huai

Authors
  1. Wei Xu
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  2. Qing Yang
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  3. Huyin Huai
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  4. Aizhong Liu
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Corresponding author

Correspondence to Aizhong Liu.

Additional information

Communicated by Y. Tsumura

Electronic supplementary material

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Table 1

(Electronic supplementary material) Location and the original source of tung trees in the accession collection of this study. All tung tree accessions were planted at XTBG, and their voucher specimens were deposited at XTBG Herbarium (DOC 57 kb)

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Xu, W., Yang, Q., Huai, H. et al. Development of EST-SSR markers and investigation of genetic relatedness in tung tree. Tree Genetics & Genomes 8, 933–940 (2012). https://doi.org/10.1007/s11295-012-0481-z

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  • DOI: https://doi.org/10.1007/s11295-012-0481-z

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