Abstract
Objectives
To evaluate the inhibitory effects of maritime pine bark extract (Pycnogenol®) on the deterioration of bone mineral density (BMD) and trabecular structure due to osteoporosis in ovariectomized (OVX) mice.
Materials and methods
Five-week-old OVX ICR mice were divided into three groups: (1) OVX mice given Pycnogenol (Pycnogenol), (2) sham-operated mice (sham), and OVX mice not given Pycogenol (OVX control). All mice received standard feed; drinking water was provided ad libitum, with tap water for the sham and OVX control groups, and water containing Pycnogenol (120 mg/L) for the Pycnogenol group. Mice were housed for 3 months under these conditions, and then the femurs were resected and blood samples collected. The BMD of the distal femoral epiphysis was analyzed by peripheral quantitative computed tomography. Micro-computed tomography was also performed to evaluate the three-dimensional structure. Deterioration of BMD and trabecular structure was compared between the groups.
Results
The Pycnogenol group showed a reduced loss of BMD compared to the OVX control group, which led to a significantly higher trabecular BMD in the former group. Additionally, surface area, number, content and complexity of the trabeculae, intertrabecular distance, and trabecular connectivity were all preserved in the Pycnogenol group. Pycnogenol thus significantly prevented trabecular architectural deterioration.
Conclusions
Our findings suggest that Pycnogenol may be useful in preventing BMD loss and trabecular architectural deterioration in osteoporosis.
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References
Drehsen G. From ancient pine bark uses to Pycnogenol. In: Packer L, Miramatzu M, Yoshikawa T, editors. Antioxidant food supplements in human health. New York: Academic Press; 1999. p. 311–22.
Packer L, Rimbach G, Virgili F. Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (Pinus maritime) bark, Pycnogenol. Free Radic Biol Med. 1999;27:704–24.
Rohdewald P. A review of the French maritime pine bark extract (Pycnogenol®), a herbal medication with a diverse clinical pharmacology. Int Clin Pharmacol Ther. 2002;40:158–68.
Hosseini S, Pishnamazi S, Sadrzadeh SMH, Farid R, Watson RR. Pycnogenol® in the management of asthma. J Med Food. 2001;4:201–9.
Lau BHS, Riesen SK, Truong KP, Lau EW, Rohdewald P, Barreta RA. Pycnogenol® as an adjunct in the management of childhood asthma. J Asthma. 2004;41:825–32.
Sime S, Reeve VE. Protection from inflammation, immunosuppression and carcinogenesis induced by UV radiation in mice by topical Pycnogenol. Photochem Photobiol. 2004;79:193–8.
Cisar P, Jany R, Waczulikova I, Sumegova K, Muchova J, Vojtassak J, et al. Effect of pine bark extract (Pycnogenol®) on symptoms of knee osteoarthritis. Phytother Res. 2008;22:1087–92.
Grimm T, Schäfer A, Högger P. Antioxidant activity and inhibition of matrix metalloproteinases by metabolites of maritime pine bark extract (Pycnogenol®). Free Radic Biol Med. 2004;36:811–22.
Grimm T, Chovanová Z, Muchová J, Sumegova K, Liptakova A, Durackova A, et al. Inhibition of NF-kB activation and MMP-9 secretion by plasma of human volunteers after ingestion of maritime pine bark extract (Pycnogenol®). J Inflamm. 2006;3:1–6.
Gack S, Vallon R, Schmidt J, Grigoriadis A, Tuckermann J, Schenkel J, et al. Expression of interstitial collagenase during skeletal development of the mouse is restricted to osteoblast-like cells and hypertrophic chondrocytes. Cell Growth Differ. 1995;6:759–67.
Johansson N, Saarialho-Kere U, Airoka K, Herva R, Nissinen L, Westermarck J, et al. Collagenase-3 (MMP-13) is expressed by hypertrophic chondrocytes, periosteal cells, and osteoblasts during human fetal bone development. Dev Dyn. 1997;208:387–97.
Chin JR, Werb Z. Matrix metalloproteinases regulate morphogenesis, migration and remodeling of epithelium, tongue skeletal muscle and cartilage in the mandibular arch. Development. 1997;124:1519–30.
Tezuka K, Nemoto K, Tezuka Y, Sato T, Ikeda Y, Kobori M, et al. Identification of matrix metalloproteinase 9 in rabbit osteoclasts. J Biol Chem. 1994;269:1506–9.
Katsunuma N. Molecular mechanisms of bone collagen degradation in bone resorption. J Bone Miner Metab. 1997;15:1–8.
Tezuka K, Tezuka Y, Maejima A, Sato T, Nemoto K, Kamioka Y, et al. Molecular cloning of a possible cysteine proteinase predominantly expressed in osteoclasts. J Biol Chem. 1994;269:1106–9.
Ferreti JL. Peripheral quantitative computed tomography for evaluating structural and mechanical properties of small bone. In: An YH, Draughn RA, editors. Mechanical testing of bone and the bone-implant interface. Boca Raton: CRC Press; 2000. p. 390–2.
RATOC System Engineering Co. Ltd. TRI/3D-BON. Basic operation manual. Tokyo, Japan: RATOC System Engineering Co Ltd. 2002.
Parfitt AM, Matthews CHE, Villanueva AR, Kleerekoper M, Frame B, Rao DS. Relationships between surface, volume, and thickness of iliac trabecular bone in aging and in osteoporosis. J Clin Invest. 1983;72:1396–409.
Odgaad A. Three-dimensional methods fore quantification of cancellous bone architecture. Bone. 1997;20:315–28.
Feldkamp LA, Goldstein SA, Parfitt AM, Jesion G, Kleerekoper M. The direct examination of three-dimensional bone architecture in vitro by computed tomography. J Bone Miner Res. 1989;4:3–11.
Ikuta A, Kumasaka S, Kashima I. Quantitative analysis using the star volume method applied to skeleton patterns extracted with a morphological filter. J Bone Miner Metab. 2000;18:271–7.
Kumasaka S, Kiyohara S, Takahashi T, Asai H, Kashima I. Morphologically extracted trabecular skeleton superimposed upon digital radiograph structure. J Bone Miner Metab. 2000;18:208–11.
Vesterby A, Gunndersen HJG, Melsen F. Star volume of marrow space and trabeculae of the first lumber vertebra: sampling efficiency and biological variation. Bone. 1989;10:7–13.
Nakamura K, Matsubara M, Asai H, Koyama A, Fujikawa T, Kashima I. Mathematical morphology for extraction of bone trabecural pattern: preliminary investigation of quantitative analysis using the star volume. J Jpn Soc Bone Morphom. 1999;9:45–51.
Garrahan NJ, Mellish RW, Compston JE. A new method for the two-dimensional analysis of bone structure in human iliac crest biopsies. J Microsc. 1986;142:341–9.
Croucher PI, Garrahan NJ, Compston JE. Assessment of cancellous bone structure: comparison of strut analysis, trabecular bone pattern factor, and marrow space star volume. J Bone Miner Res. 1996;11:955–61.
Sinaki M, Itoi E, Wahner HW, Wollan P, Gelzcer R, Mullan BP, et al. Stronger back muscles reduce the incidence of vertebral fractures; a prospective 10 year follow-up of postmenopausal women. Bone. 2002;30:836–41.
Steiner E, Jergas M, Genant H. Radiology of osteoporosis. In: Marcus R, Feldman D, Kelsey J, editors. osteoporosis. San Diego: Academic Press; 1996. p. 1019–54.
Arnold JS. Trabecular patterns and shapes in aging and osteoporosis. Metab Bone Dis Rel Res. 1980;2S:297–308.
Mosekilde L. Age-related changes in vertebral trabecular bone architecture assessed by a new method. Bone. 1988;9:247–50.
Thomsen JS, Ebbesen EN, Mosekilde LI. Age-related differences between thinning of horizontal and vertical trabeculae in human lumbar bone as assessed by a new computerized method. Bone. 2002;31:136–42.
Singh YM, Nagrath AR, Maini PS. Changes in trabecular pattern of the upper end of the femur as an index of osteoporosis. J Bone Joint Surg Am. 1970;52:457–67.
Ciarelli TE, Fyhrie DP, Schaffler MB, Goldstein SA. Variations in three-dimensional cancellous bone architecture of the proximal femur in female hip fractures and in controls. J Bone Miner Res. 2000;15:32–40.
Homminga J, McCreadie BR, Ciarelli TE, Weinans H, Goldstein SA, Huiskes R. Cancellous bone mechanical properties from normals and patients with hip fractures differ on the structure level, not on the bone hard tissue level. Bone. 2002;30:759–64.
Ito M, Ikeda K, Nishiguchi M, Shindo H, Uetani M, Hosoi T, et al. Multi-detector-row CT imaging of vertebral microstructure for evaluation of facture risk. J Bone Miner Res. 2005;20:1828–36.
Kinney JH, Ladd AJ. The relationship between three-dimensional connectivity and the elastic properties of trabecular bone. J Bone Miner Res. 1998;13:839–45.
Dempster DW. Exploiting and bypassing the bone remodeling cycle to optimize the treatment of osteoporosis. J Bone Miner Res. 1997;12:1152–4.
Tsugawa N, Shiraki M, Suhara Y, Kamao M, Tanaka K, Okano T. Vitamin K status of healthy Japanese women; age-related vitamin K requirement for γ-carboxylation of osteocalcin. Am Clin Nutr. 2006;83:380–6.
Ichikawa T, Horie IK, Ikeda K, Blumberg B, Inoue S. Steroid and xenobiotic receptor SXR mediates vitamin K2-activated transcription of extracellular matrix-related genes and collagen accumulation in osteoblastic cells. J Biol Chem. 2006;281:16927–34.
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Takano, T., Kozai, Y., Kawamata, R. et al. Inhibitory effect of maritime pine bark extract (Pycnogenol®) on deterioration of bone structure in the distal femoral epiphysis of ovariectomized mice. Oral Radiol 27, 8–16 (2011). https://doi.org/10.1007/s11282-010-0052-7
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DOI: https://doi.org/10.1007/s11282-010-0052-7