Abstract
Cariogenic biofilms have a matrix rich in exopolysaccharides (EPS), mutans and dextrans, that contribute to caries development. Although several physical and chemical treatments can be employed to remove oral biofilms, those are only partly efficient and use of biofilm-degrading enzymes represents an exciting opportunity to improve the performance of oral hygiene products. In the present study, a member of a glycosyl hydrolase family 66 from Flavobacterium johnsoniae (FjGH66) was heterologously expressed and biochemically characterized. The recombinant FjGH66 showed a hydrolytic activity against an early EPS-containing S. mutans biofilm, and, when associated with a α-(1,3)-glucosyl hydrolase (mutanase) from GH87 family, displayed outstanding performance, removing more than 80% of the plate-adhered biofilm. The mixture containing FjGH66 and Prevotella melaninogenica GH87 α-1,3-mutanase was added to a commercial mouthwash liquid to synergistically remove the biofilm. Dental floss and polyethylene disks coated with biofilm-degrading enzymes also degraded plate-adhered biofilm with a high efficiency. The results presented in this study might be valuable for future development of novel oral hygiene products.
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Funding
Maria Júlia Pozelli Macedo is a recipient of Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) scientific initiation scholarship (grant number 2023/10037-0), Pedro Ricardo Vieira Hamann is recipient of FAPESP postdoctoral fellowship (grant number 2023/07897-8), Igor Polikarpov is recipient of Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grants # 306852/2021-7 and 440180/2022-8 and FAPESP grant number 2021/08780-1.
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M.J.P.M. did experimental work, data acquisition, data analysis and original draft writing. M.Q. concieved study conception and design, establishing S. mutans biofilm growth protocols. A.N.G.D. did bioinformatic analysis and primer design. A.P.R.F. participated in study conception and design, and contributed S. mutans strain, establishing biofilms growth protocols. P.R.V.H. contributed with the study conception and design, original draft writing, data acquisition, data analysis. I.P. participated in study conception and design, data analysis, data verification, manuscript writing, supervision, and grant acquisition. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Pozelli Macedo, M.J., Xavier-Queiroz, M., Dabul, A.N.G. et al. Biochemical properties of a Flavobacterium johnsoniae dextranase and its biotechnological potential for Streptococcus mutans biofilm degradation. World J Microbiol Biotechnol 40, 201 (2024). https://doi.org/10.1007/s11274-024-04014-x
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DOI: https://doi.org/10.1007/s11274-024-04014-x