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Characterization and seasonal variation in biofilms attached to leaves of submerged plant

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Abstract

The microorganisms and functional predictions of leaf biofilms on submerged plants (Vallisneria natans (Val)) and in water samples (surface water (S) and bottom water (B)) in different seasons were evaluated in this study. S and B groups had 3249 identical operational taxonomic units (OTUs) (50.03%), while the Val group only had 1201 (18.49%) unique OTUs. There was significant overlap between microbial communities of S and B groups in the same season, while Val group showed the greater diversity. The dominant microbial clades were Proteobacteria (18.2-47.3%), Cyanobacteria (3.74-39.3%), Actinobacteria (1.64-29.3%), Bacteroidetes (1.31-21.7%), and Firmicutes (1.10-15.72%). Furthermore, there was a significant relationship between total organic carbon and the distribution of microbial taxa (p = 0.047), and TN may have altered the status of Cyanobacteria by affecting its biological nitrogen fixation capacity and reproductive capacity. The correlation network analysis results showed that the whole system consisted of 249 positive correlations and 111 negative correlations, indicating strong interactions between microbial communities. Functional predictions indicated that microbial functions were related to seasonal variation. These findings would guide the use of submerged plants to improve the diversity and stability of wetland microbial communities.

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Data Availability

The data used to support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

This work was sponsored by Natural Science Foundation of Shanghai (22ZR1446500), Open Foundation of the Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration (SHUES2022A01) and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. Department of Environmental Science and Engineering, Fudan Unersity, Shanghai, 200433, P.R. China

    Jiawei Zhang, Kexuan Liu, Yaguang Li & Jibiao Zhang

  2. Shanghai Shifang Ecology and Landscape Co., Ltd, Shanghai, 200233, P.R. China

    Jiawei Zhang, Yaguang Li & Jibiao Zhang

  3. School of Ecological and Environmental Science, Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, Institute of Eco-Chongming, East China Normal University, Shanghai, 200241, P.R. China

    Hong Deng

  4. Department of Chemistry, Fudan University, Shanghai, 200433, P.R. China

    Deying Huang

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Contributions

Jiawei Zhang: Conducting experiments, Data Analysis, Writing- Original draft preparation. Kexuan Liu: Investigation, Data Analysis. Yaguang Li: Data curation, Investigation. Hong Deng: Resources. Deying Huang: Methodology, Conducting experiments, Writing- Reviewing and Editing. Jibiao Zhang: Conceptualization, Supervision.

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Zhang, J., Liu, K., Li, Y. et al. Characterization and seasonal variation in biofilms attached to leaves of submerged plant. World J Microbiol Biotechnol 40, 19 (2024). https://doi.org/10.1007/s11274-023-03832-9

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