Abstract
Serious infections caused by Pseudomonas aeruginosa are usually related to quorum sensing (QS)-dependent virulence factors. Hence, QS inhibition is a promising approach to overcoming P. aeruginosa infections. This study aimed to investigate the effect of naproxen on biofilm formation and QS-related virulence traits of P. aeruginosa. Furthermore, the anti-QS potential of naproxen was evaluated using real-time PCR and molecular docking analysis. Our findings supported the anti-QS activity of naproxen, as evidenced by down-regulation of the lasI and rhlI genes expression as well as the attenuation of bacterial protease, hemolysin, pyocyanin, biofilm, and motility. Additionally, the high binding affinity of naproxen with QS regulatory proteins was determined in the molecular docking simulation. Altogether, these findings suggest that naproxen has a promising potential in inhibiting QS-associated traits of P. aeruginosa.
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The majority of the data used to support the findings of this study were included in the manuscript. In addition, the additional data are available from the corresponding author upon reasonable request.
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FE, DR, and Hossein Zahmatkesh: study design, lab experiments, computational analysis, data analysis, writing-original draft, writing & editing. Hojjatolah Zamani: supervision, study design, technical assistance, writing, verification, review & editing.
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Esnaashari, F., Rostamnejad, D., Zahmatkesh, H. et al. In vitro and in silico assessment of anti-quorum sensing activity of Naproxen against Pseudomonas aeruginosa. World J Microbiol Biotechnol 39, 244 (2023). https://doi.org/10.1007/s11274-023-03690-5
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DOI: https://doi.org/10.1007/s11274-023-03690-5