Abstract
Biogas, produced in anaerobic digestion, is a sustainable alternative for generating energy from agro-industrial and municipal waste. Information from the microbiota active in the process expands the possibilities for technological innovation. In this study, taxonomic annotations, and functional prediction of the microbial community of the inoculum of two processes were carried out: an industrial unit (pilot-scale urban solid waste plant—IU) and a laboratory-scale reactor fed with swine and cattle waste (LS). The biochemical potential of biogas was obtained using tested inoculum with microcrystalline cellulose, obtaining 682 LN/kgVS (LSC—laboratory scale inoculum and microcrystalline cellulose), and 583 LN/kgVS (IUC—industrial unit inoculum and microcrystalline cellulose), which is equivalent to a recovery of 91.5% of total biogas to LSC. The phyla Synergistota and Firmicutes were more abundant in LS/LSC. In the IU/IUC (treatment of restaurant waste and customs seizures), there was a greater microbiological variety and a predominance of the Bacteroidota, Cloacimonadota, Firmicutes and Caldatribacteriota. The genus Methanosaeta predominated in the process, and it was possible to infer the genes (K01895, K00193 and K00625) related to acetoclastic pathway, as well as endoglucanases that are involved in the metabolism of cellulose (LSC). Terpenoids, polyketides, cofactors, and vitamin metabolism were higher in reactors that received different substrates (IU; IUC). The taxonomic and functional differences revealed the importance of determining the microbiota in the analysis of the potential of an inoculum, combined with the use of microcrystalline cellulose, which can provide optimization information in the production of clean energy.
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Acknowledgements
We thank the International Center for Renewable Energies—Biogás supported by Itaipu Binacional (supported by Itaipu Technological Park Foundation) and EDITAL 105/2020/PRPPG-Priority Latin America and the Caribbean.
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This work was supported by EDITAL 105/2020/PRPPG-Priority Latin America and the Caribbean.
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FNL analyze the production of biogas and wrote the paper; MRZP analyzed the data and wrote the paper; FEV analyzed the microbiological data and wrote the paper; JG bioinformatics analysis; JGS analyzes of the results and wrote the paper; RFM carried out the experiments and performed analyses; SP wrote the paper and supervised the research. All authors read and approved the final manuscript.
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Luiz, F.N., Passarini, M.R.Z., Magrini, F.E. et al. Metataxonomic characterization of the microbial community involved in the production of biogas with microcrystalline cellulose in pilot and laboratory scale. World J Microbiol Biotechnol 39, 184 (2023). https://doi.org/10.1007/s11274-023-03573-9
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DOI: https://doi.org/10.1007/s11274-023-03573-9