Abstract
Aiming to broaden the base of knowledge about wild yeasts, four new indigenous strains were isolated from corn residues, and phylogenetic-tree assemblings on ITS and LSU regions indicated they belong to Meyerozyma caribbica. Yeasts were cultivated under full- and micro-aerobiosis, starting with low or high cell-density inoculum, in synthetic medium or corn hydrolysate containing glucose and/or xylose. Cells were able to assimilate both monosaccharides, albeit by different metabolic routes (fermentative or respiratory). They grew faster in glucose, with lag phases ~ 10 h shorter than in xylose. The hexose exhaustion occurred between 24 and 34 h, while xylose was entirely consumed in the last few hours of cultivation (44–48 h). In batch fermentation in synthetic medium with high cell density, under full-aerobiosis, 18–20 g glucose l−1 were exhausted in 4–6 h, with a production of 6.5–7.0 g ethanol l−1. In the xylose medium, cells needed > 12 h to consume the carbohydrate, and instead of ethanol, cells released 4.4–6.4 g l−1 xylitol. Under micro-aerobiosis, yeasts were unable to assimilate xylose, and glucose was more slowly consumed, although the ethanol yield was the theoretical maximum. When inoculated into the hydrolysate, cells needed 4–6 h to deplete glucose, and xylose had a maximum consumption of 57%. Considering that the hydrolysate contained ~ 3 g l−1 acetic acid, it probably has impaired sugar metabolism. Thus, this study increases the fund of knowledge regarding indigenous yeasts and reveals the biotechnological potential of these strains.
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Acknowledgements
This work was supported in part by grants and fellowships from the Brazilian National Council for Scientific and Technological Development—CNPq (Grant Numbers 454215/2014-2, 303735/2018-0, 305258/2018-4 and 308389/2019-0), the Santa Catarina State Research and Innovation Support Foundation—FAPESC (Grant Number 749/2016; T.O. 2016TR2188), and the Research Promotion Program from Federal University of Fronteira Sul—UFFS (Grant Numbers PES-2019-0638 and PES-2020-0213). VT, LMM, ETB, LB, and AD are grateful to the Support Program for Scientific and Technological Initiation from Federal University of Fronteira Sul (PRO-ICT/UFFS), and CM to the Coordination for the Improvement of Higher Education Personnel (CAPES/PNPD, No. 88887.352933/2019-00), for scholarships.
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VT, LMM, EVT and CWB performed the yeast isolation, the cellular growths and the batch fermentations. LB and AD performed the pretreatment and the hydrolysis of corn biomass. RH sequenced the ITS region of the strains. OF carried out the HPLC analysis. CM analyzed the data and contributed new methods. GMM, JPB, HT, BUS and SLAJ participated in designing the study and provided financial support. SLAJ also assembled the phylogenetic trees and wrote the manuscript, which was revised and approved by all authors.
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Tadioto, V., Milani, L.M., Barrilli, É.T. et al. Analysis of glucose and xylose metabolism in new indigenous Meyerozyma caribbica strains isolated from corn residues. World J Microbiol Biotechnol 38, 35 (2022). https://doi.org/10.1007/s11274-021-03221-0
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DOI: https://doi.org/10.1007/s11274-021-03221-0