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Effect of tebuconazole and trifloxystrobin on Ceratocystis fimbriata to control black rot of sweet potato: processes of reactive oxygen species generation and antioxidant defense responses

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Abstract

Black rot, caused by Ceratocystis fimbriata, is one of the most destructive disease of sweet potato worldwide, resulting in significant yield losses. However, a proper management system can increase resistance to this disease. Therefore, this study investigated the potential of using tebuconazole (TEB) and trifloxystrobin (TRI) to improve the antioxidant defense systems in sweet potato as well as the inhibitory effects on the growth of and antioxidant activity in C. fimbriata. Four days after inoculating cut surfaces of sweet potato disks with C. fimbriata, disease development was reduced by different concentrations of TEB + TRI. Infection by C. fimbriata increased the levels of hydrogen peroxide (H2O2), malondialdehyde (MDA), and electrolyte leakage (EL), and the activity of lipoxygenase (LOX) by 138, 152, 73, and 282%, respectively, in sweet potato disks, relative to control. In the sweet potato disks, C. fimbriata reduced the antioxidant enzyme activities as well as the contents of ascorbate (AsA) and reduced glutathione (GSH) by 82 and 91%, respectively, compared with control. However, TEB + TRI reduced the oxidative damage in the C. fimbriata-inoculated sweet potato disks by enhancing the antioxidant defense systems. On the other hand, applying TEB + TRI increased the levels of H2O2, MDA, and EL, and increased the activity of LOX in C. fimbriata, in which the contents of AsA and GSH decreased, and therefore, inhibited the growth of C. fimbriata. These results suggest that TEB + TRI can significantly control black rot disease in sweet potato by inhibiting the growth of C. fimbriata.

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Acknowledgements

This study was funded by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. Thanks to Dennis Murphy, Ehime University, Japan for English language correction of the manuscript.

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Authors and Affiliations

  1. Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan

    Sayed Mohammad Mohsin & Masayuki Fujita

  2. Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Sayed Mohammad Mohsin

  3. Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Mirza Hasanuzzaman

  4. Department of Horticulture, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Khursheda Parvin

  5. Laboratory of Crop Husbandry, Department of Bioresource Production Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan

    Masahiro Morokuma

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  1. Sayed Mohammad Mohsin
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  2. Mirza Hasanuzzaman
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Contributions

SMM designed, conceived, and executed the experiment and drafted the manuscript; MH designed the experiment, analyzed the data, and edited the manuscript; KP actively participated in the experiment; MM edited and reviewed the manuscript; MF supervised the experiment, and edited and reviewed the manuscript.

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Correspondence to Masayuki Fujita.

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Mohsin, S.M., Hasanuzzaman, M., Parvin, K. et al. Effect of tebuconazole and trifloxystrobin on Ceratocystis fimbriata to control black rot of sweet potato: processes of reactive oxygen species generation and antioxidant defense responses. World J Microbiol Biotechnol 37, 148 (2021). https://doi.org/10.1007/s11274-021-03111-5

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