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Pseudomonas sp. UW4 acdS gene promotes primordium initiation and fruiting body development of Agaricus bisporus

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Abstract

To simplify industrial mushroom cultivation, we introduced a bacterial Pseudomonas sp. UW4 acdS gene, encoding 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (AcdS), into fungus Agaricus bisporus. Transformant A. bisporus-acdS14 cased with sterilized-vermiculite generated primordia 5 days sooner than wild-type strain, confirming the specific role of the AcdS enzyme. Being consistent with the AcdS enzyme activity increased by 84%, the mycelium growth rate was increased by 25%; but, the ACC and ethylene concentrations were reduced by 71% and 36%, respectively, in the A. bisporus-acdS14 transformant. And the bacterium P. sp. UW4 attachment on the mycelium of the A. bisporus-acdS14 transformant was drastically reduced. We conclude that the heterogeneously expressed bacterial acdS gene degrades ACC and reduces ethylene-synthesis, eliminating ethylene inhibition on the mycelium growth and primordium formation in A. bisporus. Our results provide new insights into the mechanism underlying casing soil bacterium, and help formulate a casing-less cultivation for the next-generation mushroom industry.

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Acknowledgements

This work was supported by the National Spark Program of China (Grant No. 2015GA750013).

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, 135 Xingang Xi Road, Guangzhou, 510275, People’s Republic of China

    Chaohui Zhang, Fanmei Meng & Yuncan Ai

  2. Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural University, No. 63 Nongye Road, 450002, Henan, People’s Republic of China

    Tao Li, Yuqian Gao & Liyou Qiu

  3. College of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, People’s Republic of China

    Chaohui Zhang & Guang Zhang

  4. Zhoukou Academy of Agricultural Sciences, Zhoukou, 466000, People’s Republic of China

    Yamei Wen

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  1. Chaohui Zhang
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  2. Guang Zhang
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  3. Yamei Wen
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  4. Tao Li
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  7. Liyou Qiu
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  8. Yuncan Ai
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Correspondence to Liyou Qiu or Yuncan Ai.

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Zhang, C., Zhang, G., Wen, Y. et al. Pseudomonas sp. UW4 acdS gene promotes primordium initiation and fruiting body development of Agaricus bisporus. World J Microbiol Biotechnol 35, 163 (2019). https://doi.org/10.1007/s11274-019-2741-7

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  • DOI: https://doi.org/10.1007/s11274-019-2741-7

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