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Oxidative damage induced by H2O2 reveals SOS adaptive transcriptional response of Dietzia cinnamea strain P4

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Abstract

The oxidative stress response of the highly resistant actinomycete Dietzia cinnamea P4 after treatment with hydrogen peroxide (H2O2) was assessed in order to depict the possible mechanisms underlying its intrinsic high resistance to DNA damaging agents. We used transcriptional profiling to monitor the magnitude and kinetics of changes in the mRNA levels after exposure to different concentrations of H2O2 at 10 min and 1 h following the addition of the stressor. Catalase and superoxide dismutase genes were induced in different ways, according to the condition applied. Moreover, alkyl hydroperoxide reductase ahpCF, thiol peroxidase, thioredoxin and glutathione genes were upregulated in the presence of H2O2. Expression of peroxidase genes was not detected during the experiment. Overall results point to an actinomycete strain endowed with a set of enzymatic defenses against oxidative stress and with the main genes belonging to a functional SOS system (lexA, recA, uvrD), including suppression of lexA repressor, concomitantly to recA and uvrD gene upregulation upon H2O2 challenge.

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Authors and Affiliations

  1. Microbial Corrosion Laboratory, Universidade Estácio de Sá, Bispo Street, 83, Rio Comprido, Rio de Janeiro, RJ, CEP 20261-063, Brazil

    Luciano Procópio

  2. Laboratório de Diagnóstico Molecular e Hematologia, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Marcelo Pádula

  3. Microbial Ecology Laboratory, Goningen University, Groningen, The Netherlands

    Jan Dirk van Elsas

  4. Laboratório de Genética Microbiana, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Lucy Seldin

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  1. Luciano Procópio
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  2. Marcelo Pádula
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  3. Jan Dirk van Elsas
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  4. Lucy Seldin
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Correspondence to Luciano Procópio.

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Procópio, L., Pádula, M., van Elsas, J.D. et al. Oxidative damage induced by H2O2 reveals SOS adaptive transcriptional response of Dietzia cinnamea strain P4. World J Microbiol Biotechnol 35, 53 (2019). https://doi.org/10.1007/s11274-019-2628-7

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  • DOI: https://doi.org/10.1007/s11274-019-2628-7

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