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Activation of phenylpropanoid pathway and PR of potato tuber against Fusarium sulphureum by fungal elicitor from Trichothecium roseum

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Abstract

The induced resistance of potato tuber (Solanum tuberosum cv. Xindaping) tissue against Fusarium sulphureum by a fungal elicitor from the incompatible pathogen Trichothecium roseum and its possible mechanism were studied. The results showed that the lesion development of the wound-inoculated potato tuber was significantly reduced by treatment with the fungal elicitor from T. roseum (P < 0.05). Inoculation with F. sulphureum on the 16th day after treatment with the fungal elicitor80 at 15.0 μg/ml had the best resistant effect in the potato tuber, with the diameter being only reduced by 47 % that of the control. In addition, the results also showed that the potato tuber treated with the fungal elicitor80 could systemically induce lignin deposition, total phenolic content, flavonoid content and defense enzymes, including three keys phenylpropanoid pathway (PAL, 4CL and C4H) and pathogenesis-related (GLU and CHT) enzymes. The fungal elicitor80 also enhanced the up-regulation of the transcription and expression of PAL, C4H, 4CL, GLU and CHT genes. The treatment with the fungal elicitor80 + F. sulphureum caused the marked and/or prompt enhancement of all indexes when compared to treatment with the fungal elicitor80 or inoculation with the pathogen alone. The results suggested that the fungal elicitor of T. roseum could significantly enhance defense responses in potato tuber against dry rot mainly due to the up-regulation of the transcription and expression of resistance-related genes as well as increasing the activity of resistance-related enzymes and antifungal compounds.

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References

  • Abeles FB, Forrence LE (1979) Temporal and hormonal control of β-1, 3-glucanase in Phaseolus vulgaris L. Plant Physiol 45:395–400

    Article  Google Scholar 

  • Adam AL, Pike S, Hoyos ME, Stone JM, Walker JC, Novacky AR (1997) Rapid and transient activation of a myelin basic protein kinase in tobacco leaves treated with harpin from Erwinia amylovora. Plant Physiol 115:853–861

    CAS  Google Scholar 

  • Allen RL, Bittner-Eddy PD, Grenville-Briggs LJ, Meitz JC, Rehmany AP, Rose LE, Beynon JL (2004) Host-parasite coevolutionary conflict between Arabidopsis and downy mildew. Science 306:1957–1960

    Article  CAS  Google Scholar 

  • Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399

    Article  CAS  Google Scholar 

  • Bao G, Bi Y, Li Y, Kou ZH, Hu LG, Ge YH, Wang Y (2014) Overproduction of reactive oxygen species involved in the pathogenicity of Fusarium in potato tubers. Physiol Mol Plant Pathol 86:35–42

    Article  CAS  Google Scholar 

  • Bi Y, Tian SP, Liu HX, Zhao J, Cao JK, Li YC, Zhang WY (2003) Effect of temperature on chilling injury, decay and quality of Hami melon during storage. Postharvest Biol Technol 29:229–232

    Article  Google Scholar 

  • Bi Y, Tian SP, Zhao J, Ge YH (2005) Harpin induces local and systemic resistance against Trichothecium roseum in harvested Hami melons. Postharvest Biol Technol 38:183–187

    Article  Google Scholar 

  • Boller T, Gehri A, Mauch F, Vögeli U (1983) Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function. Planta 157:22–31

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Bu B, Qiu D, Zeng H (2014) A fungal protein elicitor PevD1 induces Verticillium wilt resistance in cotton. Plant Cell Rep 33:461–470

    Article  CAS  Google Scholar 

  • Chen M, Zeng H, Qiu D, Guo L, Yang X, Shi H, Zhou T, Zhao J (2012) Purification and characterization of a novel hypersensitive response-inducing elicitor from Magnaporthe oryzae that triggers defense response in rice. PLoS One 7:e37654

    Article  CAS  Google Scholar 

  • De W, Pierre JGM, Mehrabi R, Van D, Burg HA, Stergiopoulos I (2009) Fungal effector proteins: past, present and future. Mol Plant Pathol 10:735–747

    Article  Google Scholar 

  • Desjardins AE, Christ-Harned EA, McCormick SP, Secor GA (1993) Population structure and genetic analysis of field resistance to thiabendazole in gibberella pulicaris from potato tuber. Phytopathology 83:164–170

    Article  CAS  Google Scholar 

  • Dixon RA, Harrison MJ, Lamb CJ (1994) Early events in the activation of plant defense responses. Annu Rev Phytopathol 32:479–501

    Article  CAS  Google Scholar 

  • Droby S, Wisniewski M, Macarisin D, Wilson C (2009) Twenty years of postharvest biocontrol research: is it time for a new paradigm. Postharvest Biol Technol 52:137–145

    Article  Google Scholar 

  • Durrant WE, Dong X (2004) Systemic acquired resistance. Annu Rev Phytopathol 42:185–209

    Article  CAS  Google Scholar 

  • Ebel J, Mithöfer A (1998) Early events in the elicitation of plant defence. Planta 206:335–348

    Article  CAS  Google Scholar 

  • Ellis JG, Rafiqi M, Gan P, Chakrabarti A, Dodds PN (2009) Recent progress in discovery and functional analysis of effector proteins of fungal and oomycete plant pathogens. Curr Opin Plant Biol 12:399–405

    Article  CAS  Google Scholar 

  • Foyer CH, Noctor G (2005) Oxidant and antioxidant signaling in plants: a reevaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ 28:1056–1071

    Article  CAS  Google Scholar 

  • Garcia-Brugger A, Lamotte O, Vandelle E, Bourque S, Lecourieux D (2006) Early signaling events induced by elicitors of plant defenses. Mol Plant Microbe Interact 19:711–724

    Article  CAS  Google Scholar 

  • Glazebrook J (2005) Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu Rev Phytopathol 43:205–227

    Article  CAS  Google Scholar 

  • Hammerschmidt R (2008) Challenge inoculation reveals the benefits of resistance priming. Physiol Mol Plant Pathol 73:59–60

    Article  Google Scholar 

  • Hammond-Kosack KE, Parker JE (2003) Deciphering plant-pathogen communication: fresh perspectives for molecular resistance breeding. Curr Opin Biotechnol 14:177–193

    Article  CAS  Google Scholar 

  • Huang J, Gu M, Lai Z, Fan B, Shi K, Zhou YH, Yu JQ, Chen Z (2010) Functional analysis of the Arabidopsis PAL gene family in plant growth, development, and response to environmental stress. Plant Physiol 153:1526–1538

    Article  CAS  Google Scholar 

  • Kokkonen M, Ojala L, Parikka P, Jestoi M (2010) Mycotoxin production of selected Fusarium species at different culture conditions. Int J Food Microbiol 143:17–25

    Article  CAS  Google Scholar 

  • Kulye M, Liu H, Zhang YL (2012) Hrip1 a novel protein elicitor from necrotrophic fungus, Alternaria tenuissima, elicits cell death, expression of defence-related genes and systemic acquired resistance in tobacco. Plant Cell Environ 35:2104–2120

    Article  CAS  Google Scholar 

  • Li YC, Bi Y, Ge YH, Sun XJ, Wang Y (2009a) Antifungal activity of sodium silicate on Fusarium sulphureum and its effect on dry rot of potato tubers. J Food Sci 74:213–218

    Article  CAS  Google Scholar 

  • Li YC, Bi Y, Hu J, Zhang CY (2009b) Induced resistance in potato tuber tissue against Fusarium sulphureum infection by hyphal cell wall extract from Trichothecium roseum. Acta Botanica Boreal Occident Sinica 29:0749–0753

    Google Scholar 

  • Li YC, Yin Y, Bi Y (2012) Effect of riboflavin on postharvest disease of Asia pear and the possible mechanisms involved. Phytoparasitica 40:261–268

    Article  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔC(T) method. Methods 25:402–408

    Article  CAS  Google Scholar 

  • Mao J, Liu Q, Yang X, Long C, Zhao M, Zeng H, Liu H, Yuan J, Qiu D (2010) Purification and expression of a protein elicitor from Alternaria tenuissima and elicitor-mediated defence responses in tobacco. Ann Appl Biol 156:411–420

    Article  CAS  Google Scholar 

  • Morrison IM (1972) A semi-micro method for the determination of lignin and its use in predicting the digestibility of forage crops. J Sci Food Agric 23:455–463

    Article  CAS  Google Scholar 

  • Nürnberger T (1999) Signal perception in plant pathogen defense. Cell Mol Life Sci 55:167–182

    Article  Google Scholar 

  • Peng DH, Qiu DW, Ruan LF (2011) Protein elicitor PemG1 from Magnaporthe grisea induces systemic acquired resistance (SAR) in plants. Mol Plant Microbe Interact 24:1239–1246

    Article  CAS  Google Scholar 

  • Pirie A, Mullins MG (1976) Changes in anthocyanin and phenolics content of grapevine leaf and fruit tissues treated with sucrose, nitrate and abscisic acid. Plant Physiol 58:468–472

    Article  CAS  Google Scholar 

  • Qiu D, Mao J, Yang X, Zeng H (2009) Expression of an elicitor encoding gene from Magnaporthe grisea enhances resistance against blast disease in transgenic rice. Plant Cell Rep 28:925–933

    Article  CAS  Google Scholar 

  • Rowland O, Ludwig AA, Merrick CJ, Baillieul F, Tracy FE, Durrant WE, Fritz-Laylin L, Nekrasov V, Sjolander K, Yoshioka H, Jones JD (2005) Functional analysis of Avr9/Cf-9 rapidly elicited genes identifies a protein kinase, ACIK1, that is essential for full Cf-9-dependent disease resistance in tomato. Plant Cell 17:295–310

    Article  CAS  Google Scholar 

  • Schoch GA, Nikov GN, Alworth WL, Werck-Reichhart D (2002) Chemical inactivation of the cinnamate 4-hydroxylase allows for the accumulation of salicylicacid in elicited cells. Plant Physiol 130:1022–1031

    Article  CAS  Google Scholar 

  • Silipo A, Erbs G, Shinya T, Dow JM, Parrilli M, Lanzetta R, Shibuya N, Newman MA, Molinaro A (2010) Glyco-conjugates as elicitors or suppressors of plant innate immunity. Glycobiology 20:406–419

    Article  CAS  Google Scholar 

  • Sun XJ, Li YC, Bi Y, Liu J, Yin Y (2009) The investigation and analysis of postharvest diseases of potato in northwest regions. Chin Potato J 23:364–365

    Google Scholar 

  • Sundar AR, Velazhahan R, Nagarathinam S, Vidhyasekaran P (2008) Induction of pathogenesis-related proteins in sugarcane leaves and cell-cultures by a glycoprotein elicitor isolated from Colletotrichum falcatum. Biol Plant 52:321–328

    Article  CAS  Google Scholar 

  • Terry LA, Joyce DC (2004) Elicitors of induced disease resistance in postharvest horticultural crops: a brief review. Postharvest Biol Technol 32:1–13

    Article  Google Scholar 

  • Tian SP, Wan YK, Qin GZ, Xu Y (2006) Induction of defense responses against Alternaria rot by different elicitors in harvested pear fruit. Appl Microbiol Biotechnol 70:729–734

    Article  CAS  Google Scholar 

  • Tian SP, Yao HJ, Deng X, Xu XB, Qin GZ, Chan ZL (2007) Characterization and expression of β-1,3-glucanase genes in jujube fruit induced by the microbial biocontrol agent Cryptococcus laurentii. Phytopathology 97:260–268

    Article  CAS  Google Scholar 

  • Van der Ent S, Van Hulten M, Pozo MJ, Czechowski T, Udvardi MK, Pieterse CMJ, Ton J (2009) Priming of plant innate immunity by rhizobacteria and β-aminobutyric acid: differences and similarities in regulation. New Phytol 183:419–431

    Article  Google Scholar 

  • Wang B, Yang X, Zeng H, Liu H, Zhou T, Tan B, Yuan J, Guo L, Qiu D (2012) The purification and characterization of a novel hypersensitive-like response-inducing elicitor from Verticilliumdahliae that induces resistance responses in tobacco. Appl Microbiol Biotechnol 93:91–201

    Google Scholar 

  • Wang XL, Xu F, Wang J, Jin P, Zheng YH (2013) Bacillus cereus AR156 induces resistance against Rhizopus rot through priming of defense responses in peach fruit. Food Chem 136:400–406

    Article  CAS  Google Scholar 

  • Wei ZM, Laby RJ, Zumoff CH, Bauer DW, He SY, Collmer A, Beer SV (1992) Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora. Science 257:85–88

    Article  CAS  Google Scholar 

  • Wei ZQ, Zhang TY, Du X (2006) The occurrence and control of dry rot of potato tuber. Plant Prot 32:103–105

    Google Scholar 

  • Xu L, Zhu L, Tu L, Liu L, Yuan D, Jin L, Long L, Zhang X (2011) Lignin metabolism has a central role in the resistance of cotton to the wilt fungus Verticllium dahlia as revealed by RNA-Seadependent transcriptional analysis and histochemistry. J Exp Bot 62:5607–5621

    Article  CAS  Google Scholar 

  • Yin Y, Li YC, Bi Y, Cheng SJ, Li YC, Yuan L (2010) Postharvest treatment with baminobutyric acid induces resistance against dry rot caused by Fusarium sulphureum in potato tuber. Agric Sci China 9:1372–1380

    Article  CAS  Google Scholar 

  • Yu MM, Shen L, Zhang AJ, Sheng JP (2011) Methyl jasmonate-induced defense responses are associated with elevation of 1-aminocyclopropane-1-carboxylate oxidase in Lycopersicon esculentum fruit. J Plant Physiol 168:1820–1827

    Article  CAS  Google Scholar 

  • Yun MS, Chen W, Deng F, Kiyokawa T, Mametsuka K, Yogo Y (2006) An in vitro screening assay to discover novel inhibitors of 4-coumarate: CoA ligase. Pest Manag Sci 62:1065–1071

    Article  CAS  Google Scholar 

  • Zhang W, Yang X, Qiu D, Guo L, Zeng H, Mao J, Gao Q (2011) PeaT1-induced systemic acquired resistance in tobacco follows salicylic acid-dependent pathway. Mol Biol Rep 38:2549–2556

    Article  CAS  Google Scholar 

  • Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333

    Article  CAS  Google Scholar 

  • Zhao MZ, Yang XF, Zhang M, Yuan JJ, Qiu DW (2007) Purification and bioactivities of a protein growth-activator from Aternaria tenuissima. Chin J Biol Control 23:170–173

    Google Scholar 

Download references

Acknowledgments

This research was financially supported by Special Fund for Agri-biotechnology of Gansu Provincial Department of Agriculture and Animal Husbandry (GNSW-2011-11), and“Fuxi Distinguished Young Talent Cultivation Project”of Gansu Agricultural University.

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  1. College of Food Science and Engineering, Gansu Agricultural University, No. 1 Yingmen Village, Anning District, Lanzhou, 730070, People’s Republic of China

    Xiao-yan Yu, Yang Bi, Lu Yan, Xiao Liu, Yi Wang, Ke-ping Shen & Yong-cai Li

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  1. Xiao-yan Yu
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  2. Yang Bi
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  3. Lu Yan
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Correspondence to Yong-cai Li.

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Yu, Xy., Bi, Y., Yan, L. et al. Activation of phenylpropanoid pathway and PR of potato tuber against Fusarium sulphureum by fungal elicitor from Trichothecium roseum . World J Microbiol Biotechnol 32, 142 (2016). https://doi.org/10.1007/s11274-016-2108-2

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