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Effects of variation in precipitation on the distribution of soil bacterial diversity in the primitive Korean pine and broadleaved forests

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Abstract

Patterns of precipitation have changed as a result of climate change and will potentially keep changing in the future. Therefore, it is critical to understand how ecosystem processes will respond to the variation of precipitation. However, compared to aboveground processes, the effects of precipitation change on soil microorganisms remain poorly understood. Changbai Mountain is an ideal area to study the responses of temperate forests to the variations in precipitation. In this study, we conducted a manipulation experiment to simulation variation of precipitation in the virgin, broad-leaved Korean pine mixed forest in Changbai Mountain. Plots were designed to increase precipitation by 30 % [increased (+)] or decrease precipitation by 30 % [decreased (−)]. We analyzed differences in the diversity of the bacterial community in surface bulk soils (0–5 and 5–10 cm) and rhizosphere soils between precipitation treatments, including control. Bacteria were identified using the high-throughput 454 sequencing method. We obtained a total 271,496 optimized sequences, with a mean value of 33,242 (±1,412.39) sequences for each soil sample. Being the same among the sample plots with different precipitation levels, the dominant bacterial communities were Proteobacteria, Acidobacteria, Actinobacteria, Planctomycetes, and Chloroflexi. Bacterial diversity and abundance declined with increasing soil depth. In the bulk soil of 0–5 cm, the bacterial diversity and abundance was the highest in the control plots and the lowest in plots with reduced precipitation. However, in the soil of 5–10 cm, the diversity and abundance of bacteria was the highest in the plots of increased precipitation and the lowest in the control plots. Bacterial diversity and abundance in rhizosphere soils decreased with increased precipitation. This result implies that variation in precipitation did not change the composition of the dominant bacterial communities but affected bacterial abundance and the response patterns of the dominant communities to variation in precipitation.

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Acknowledgments

The authors are grateful to the scientists at Institute of Applied Ecology, Chinese Academy of Sciences for their supporting of field study site. We thank all the faculty and staff at Changbai Mountain station for their help in field work and those who have assisted in the lab experimental and paper writing. This work was supported by the National Natural Science Foundation of China (Grant No. 41105104), National Basic Scientific Talent Training Fund Projects (Grant No. J1210053), and Fundamental Research Funds for the Central Universities (Grant No. DL10CA05).

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Authors and Affiliations

  1. Northeast Forestry University, Harbin, 150040, China

    Nannan Wang, Meiju Wang, Shilan Li & Fujuan Feng

  2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China

    Xin Sui & Shijie Han

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  1. Nannan Wang
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  2. Meiju Wang
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  3. Shilan Li
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  4. Xin Sui
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  5. Shijie Han
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Correspondence to Nannan Wang.

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Wang, N., Wang, M., Li, S. et al. Effects of variation in precipitation on the distribution of soil bacterial diversity in the primitive Korean pine and broadleaved forests. World J Microbiol Biotechnol 30, 2975–2984 (2014). https://doi.org/10.1007/s11274-014-1725-x

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  • DOI: https://doi.org/10.1007/s11274-014-1725-x

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