Abstract
Ginger (Zingiber officinale) is cultivated commercially in most parts of the world especially in India for its culinary and medicinal applications. One of the major challenges that limit the yield of ginger is rhizome rot disease caused by organisms including Pythium myriotylum. A feasible ecofriendly method is yet to be devised to prevent the plant from this threatening disease. Recent studies on plant microbiome show the possibility of having endophytic organisms with plant protective characteristics associated with the plants. Because of the uniquely evolved underground nature of the ginger rhizome and its peculiar survival in soil for a long time, many interesting endophytic microbes with plant protective characters can be well expected from it. In the current study, previously isolated endophytic Pseudomonas aeruginosa from ginger was investigated in detail for its effect on Pythium myriotylum. The rhizome protective effect of the organism was also studied by co-inoculation studies, which confirmed that Pseudomonas aeruginosa has very potent inhibitory effect on Pythium myriotylum. On further studies, the active antifungal compound was identified as phenazine 1-carboxylic acid.
References
Amith Abraham, Shaji Philip, C Kuruvilla Jacob, K Jayachandran (2013) Novel bacterial endophytes from Hevea brasiliensis as biocontrol agent against Phytophthora leaf fall disease. BioControl 58:675–684
Bano N, Musarrat J (2003) Characterization of a new Pseudomonas aeruginosa strain NJ-15 as a potential biocontrol agent. Curr Microbiol 46:324–328
Chin-A-Woeng TFC, Bloemberg GV, Bij AJ, Drift KMGF, Schripsema J, Kroon B, Scheffer RJ, Keel C, Bakker PAHM, Tichy HV, Bruijn FJ, Thomas-Oates JE, Lugtenberg BJJ (1998) Biocontrol by phenazine-1-carboxamide producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporum f sp radicis-lycopersici. Mol Plant Microbe Interact 11:1069–1077
Chin-A-Woeng TFC, Thomas-Oates JE, Lugtenberg BJJ, Bloemberg GV (2001) Introduction of the phzH gene of Pseudomonas chlororaphis PCL1391 extends the range of biocontrol ability of phenazine-1-carboxylic acid-producing Pseudomonas spp strains. Mol Plant Microbe Interact 14:1006–1015
Duffy BK, Defago G (1999) Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strain. Appl Environ Microb 65(6):2429–2438
Hallmann J, Kloepper JW, Rodriguez-Kibana R, Sikora RA (1995) Endophytic rhizobacteria as antagonists of Meloidogyne incognita on cucumber. Phytopathology 85:1136
Jasim B, Joseph AA, Jimtha John C, Mathew J, Radhakrishnan EK (2013a) Isolation and characterization of plant growth promoting endophytic bacteria from the rhizome of Zingiber officinale. Biotech 3:1–8
Jasim B, Sabu Rohini C, Anisha CJ, John JM, Radhakrishnan EK (2013b) Antifungal and plant growth promoting properties of endophytic Pseudomonas aeruginosa from Zingiber officinale. J Pure Appl Microbiol 7(2):1–7
Meena KK, Mesapogu S, Kumar M, Yandigeri MS, Singh G, Saxena AK (2010) Co-inoculation of the endophytic fungus Piriformospora indica with the phosphate-solubilising bacterium Pseudomonas striata affects population dynamics and plant growth in chickpea. Biol Fertil Soils 46(2):169–174
Minaxi SJ (2010) Characterization of Pseudomonas aeruginosa RM-3 as a potential biocontrol agent. Mycopathologia 170(3):181–193
Shanmugaiah V, Balasubramanian N, Gomathinayagam S, Monoharan PT, Rajendran A (2009) Effect of single application of Trichoderma viride and Pseudomonas fluorescens on growth promotion in cotton plants. Afr J Agric Res 4(11):1220–1225
Stead P, Rudd BB, Noble HD, Dawson MJ (1996) Induction of phenazine biosynthesis in cultures of Pseudomonas aeruginosa by L-N-(3-oxohexanoyl) homoserine lactone. FEMS Microbiol 140(1):15–22
Stone JK, Bacon CW, White JF Jr (2000) An overview of endophytic microbes: endophytism defined. In: Bacon CW, White JF (eds) Microbial endophytes. Marcel Dekker, New York, USA
Tambong JT, Hofte M (2001) Phenazines are involved in biocontrol of Pythium myriotylum on cocoyam by Pseudomonas aeruginosa PNA1. Eur J Plant Pathol 107:511–521
Thomashow LS, Weller DM (1996) Current concepts in the use of introduced bacteria for biological disease control: mechanisms and antifungal metabolites. In: Stacey K (ed) Plant–microbe interactions, vol 1. Chapman and Hall, New York
Whipps JM (1997) Interactions between fungi and plant pathogens in soil and rhizosphere. In: Gange AC, Brown VK (eds) Multitrophic interactions in terrestrial systems. Blackwell Science, Oxford
Whipps JM (2001) Microbial interactions and biocontrol in the rhizosphere. J Exp Bot 52:487–511
Acknowledgments
This study was supported by Department of Biotechnology (DBT), Government of India under DBT-RGYI support scheme, Department of Science and Technology, Government of India under DST-PURSE program and Kerala State Council for Science, Technology and Environment, Government of Kerala.
Conflict of interest
Authors declare to have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jasim, B., Anisha, C., Rohini, S. et al. Phenazine carboxylic acid production and rhizome protective effect of endophytic Pseudomonas aeruginosa isolated from Zingiber officinale . World J Microbiol Biotechnol 30, 1649–1654 (2014). https://doi.org/10.1007/s11274-013-1582-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-013-1582-z