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Characterization of the co-purified invertase and β-glucosidase of a multifunctional extract from Aspergillus terreus

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Abstract

The filamentous fungus Aspergillus terreus secretes both invertase and β-glucosidase when grown under submerged fermentation containing rye flour as the carbon source. The aim of this study was to characterize the co-purified fraction, especially the invertase activity. An invertase and a β-glucosidase were co-purified by two chromatographic steps, and the isolated enzymatic fraction was 139-fold enriched in invertase activity. SDS-PAGE analysis of the co-purified enzymes suggests that the protein fraction with invertase activity was heterodimeric, with subunits of 47 and 27 kDa. Maximal invertase activity, which was determined by response surface methodology, occurred in pH and temperature ranges of 4.0–6.0 and 55–65 °C, respectively. The invertase in co-purified enzymes was stable for 1 h at pH 3.0–10.0 and maintained full activity for up to 1 h at 55 °C when diluted in water. Invertase activity was stimulated by 1 mM concentrations of Mn2+ (161 %), Co2+ (68 %) and Mg2+ (61 %) and was inhibited by Al3+, Ag+, Fe2+ and Fe3+. In addition to sucrose, the co-purified enzymes hydrolyzed cellobiose, inulin and raffinose, and the apparent affinities for sucrose and cellobiose were quite similar (KM = 22 mM). However, in the presence of Mn2+, the apparent affinity and Vmax for sucrose hydrolysis increased approximately 2- and 2.9-fold, respectively, while for cellobiose, a 2.6-fold increase in Vmax was observed, but the apparent affinity decreased 5.5-fold. Thus, it is possible to propose an application of this multifunctional extract containing both invertase and β-glucosidase to degrade plant biomass, thus increasing the concentration of monosaccharides obtained from sucrose and cellobiose.

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Authors and Affiliations

  1. Instituto de Química de Araraquara, UNESP, Rua Professor Francisco Degni s/n, Quitandinha, Araraquara, SP, 14800-900, Brazil

    Marielle Aleixo Giraldo & Heloísa Bressan Gonçalves

  2. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, USP, Avenida Bandeirantes 3900, Monte Alegre, Ribeirão Preto, SP, 14040-901, Brazil

    Rosa dos Prazeres Melo Furriel, João Atílio Jorge & Luis Henrique Souza Guimarães

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  1. Marielle Aleixo Giraldo
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  2. Heloísa Bressan Gonçalves
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  3. Rosa dos Prazeres Melo Furriel
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  4. João Atílio Jorge
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  5. Luis Henrique Souza Guimarães
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Correspondence to Luis Henrique Souza Guimarães.

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Giraldo, M.A., Gonçalves, H.B., Furriel, R.P.M. et al. Characterization of the co-purified invertase and β-glucosidase of a multifunctional extract from Aspergillus terreus . World J Microbiol Biotechnol 30, 1501–1510 (2014). https://doi.org/10.1007/s11274-013-1570-3

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