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Leguminous plants nodulated by selected strains of Cupriavidus necator grow in heavy metal contaminated soils amended with calcium silicate

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Abstract

Increasing concern regarding mining area environmental contamination with heavy metals has resulted in an emphasis of current research on phytoremediation. The aim of the present study was to assess the efficiency of symbiotic Cupriavidus necator strains on different leguminous plants in soil contaminated with heavy metals following the application of inorganic materials. The application of limestone and calcium silicate induced a significant increase in soil pH, with reductions in zinc and cadmium availability of 99 and 94 %, respectively. In addition, improved nodulation of Mimosa caesalpiniaefolia, Leucaena leucocephala and Mimosa pudica in soil with different levels of contamination was observed. Significant increases in the nitrogen content of the aerial parts of the plant were observed upon nodulation of the root system of Leucaena leucocephala and Mimosa pudica by strain UFLA01-659 (36 and 40 g kg−1) and by strain UFLA02-71 in Mimosa caesalpiniaefolia (39 g kg−1). The alleviating effect of calcium silicate resulted in higher production of dry matter from the aerial part of the plant, an increase in nodule number and an increase in the nitrogen fixation rate. The results of the present study demonstrate that the combination of rhizobia, leguminous plants and calcium silicate may represent a key factor in the remediation of areas contaminated by heavy metals.

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Acknowledgments

We thank Fapemig, CAPES and CNPq for student fellowships, Fapemig for a research fellowship, grant and for financial support, CNPq for research fellowships and grants. This publication presents part of the findings of the project Monitoring and Recovery Strategies for Areas Impacted by Mining Activities.

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  1. Cleide Aparecida Bomfeti

    Present address: Instituto de Ciência e Tecnologia, Universidade dos Vales do Jequitinhonha e Mucuri, Teófilo Otoni, Minas Gerais, CEP 39803-371, Brazil

  2. Cláudio Roberto Fonseca de Sousa Soares

    Present address: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Trindade, Florianópolis, Santa Catarina, CEP 88040-970, Brazil

Authors and Affiliations

  1. Departamento de Ciência do Solo, Universidade Federal de Lavras, C.P.3037, Lavras, Minas Gerais, Brazil

    Paulo Ademar Avelar Ferreira, Guilherme Lopes, Cleide Aparecida Bomfeti, Silvia Maria de Oliveira Longatti, Cláudio Roberto Fonseca de Sousa Soares, Luiz Roberto Guimarães Guilherme & Fatima Maria de Souza Moreira

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  1. Paulo Ademar Avelar Ferreira
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  2. Guilherme Lopes
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  3. Cleide Aparecida Bomfeti
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Correspondence to Fatima Maria de Souza Moreira.

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Avelar Ferreira, P.A., Lopes, G., Bomfeti, C.A. et al. Leguminous plants nodulated by selected strains of Cupriavidus necator grow in heavy metal contaminated soils amended with calcium silicate. World J Microbiol Biotechnol 29, 2055–2066 (2013). https://doi.org/10.1007/s11274-013-1369-2

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  • DOI: https://doi.org/10.1007/s11274-013-1369-2

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