Skip to main content
SpringerLink
Log in

Phenotypic and molecular characterization of rhizobacterium Burkholderia sp. strain R456 antagonistic to Rhizoctonia solani, sheath blight of rice

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The effect of rhizobacterium Burkholderia sp. strain R456 on the inhibition of Rhizoctonia solani, sheath blight of rice was examined. Results from this study indicated that strain R456 not only suppressed the in vitro mycelial growth of R. solani, but also reduced the incidence and severity of rice sheath blight under greenhouse conditions. However, similar to plant pathogenic strain LMG 1222T of Burkholderia cepacia, the type species of the genus, infiltration of tobacco leaves with cell suspension of strain R456 resulted in typical hypersensitivity reactions while the two bacterial strains were unable to cause disease symptoms on rice seedlings. The fatty acid methyl ester profile, sole carbon source utilization, and biochemical tests confirmed that the antagonistic rhizobacterium R456 is a member of the genus Burkholderia. Furthermore, strain R456 was differentiated from B. cepacia LMG 1222T and was identified as Burkholderia seminalis based on recA gene sequence analysis and multilocus sequence typing. In addition, this rhizobacterium had a lower proteolytic activity compared with that of the pathogenic B. cepacia LMG 1222T while no cblA and esmR marker genes were detected for the two bacterial strains. Overall, this is the first characterization of rhizobacterium B. seminalis that protected rice seedlings from infection by R. solani.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Baldwin A, Mahenthiralingam E, Thickett KM, Honeybourne D, Maiden MCJ, Govan JR, Speert DP, LiPuma JJ, Vandamme P, Dowson CG (2005) Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex. J Clin Microbiol 43:4665–4673. doi:10.1128/JCM.43.9.4665-4673.2005

    Article  CAS  Google Scholar 

  • Chumthong A, Kanjanamaneesathian M, Pengnoo A, Wiwattanapatapee R (2008) Water-soluble granules containing Bacillus megaterium for biological control of rice sheath blight: formulation, bacterial viability and efficacy testing. World J Microbiol Biotechnol 24:2499–2507. doi:10.1007/s11274-008-9774-7

    Article  Google Scholar 

  • Coenye T, Vandamme P (2003) Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol 5:719–729. doi:10.1046/j.1462-2920.2003.00471.x

    Article  CAS  Google Scholar 

  • De Costa DM, Samarasinghe SST, Dias HRD, Dissanayake DMN (2008) Control of rice sheath blight by phyllosphere epiphytic microbial antagonists. Phytoparasitica 36:52–65

    Article  Google Scholar 

  • Fain MG, Haddock JD (2001) Phenotypic and phylogenetic characterization of Burkholderia (Pseudomonas) sp. strain LB400. Curr Microbiol 42:269–275. doi:10.1007/s002840110216

    CAS  Google Scholar 

  • Fang Y, Zhang LX, Xie GL (2007) Internal bacterial rot of onion bulbs caused by Burkholderia cepacia in China. J Plant Pathol 89:304

    Google Scholar 

  • Fang Y, Li B, Wang F, Liu BP, Wu ZY, Qiu W, Xie GL (2009) Bacterial fruit rot of apricot caused by Burkholderia cepacia in China. Plant Pathol J 25:429–432

    Article  Google Scholar 

  • Fang Y, Lou MM, Li B, Xie GL, Wang F, Zhang LX, Luo YC (2010) Characterization of Burkholderia cepacia complex from cystic fibrosis patients in China and their chitosan susceptibility. World J Microbiol Biotechnol 26:443–450. doi:10.1007/s11274-009-0187-z

    Article  CAS  Google Scholar 

  • Gingues S, Kooi C, Visser MB, Subsin B, Sokol PA (2005) Distribution and expression of the ZmpA metalloprotease in the Burkholderia cepacia complex. J Bacteriol 187:8247–8255

    Article  CAS  Google Scholar 

  • Henry DA, Mahenthiralingam E, Vandamme P, Coenye T, Speert DP (2001) Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex. J Clin Microbiol 39:1073–1078. doi:10.1128/JCM.39.3.1073-1078.2001

    Article  CAS  Google Scholar 

  • Jacobs JL, Fasi AC, Ramette A, Smith JJ, Hammerschmidt R, Sundin GW (2008) Identification and onion pathogenicity of Burkholderia cepacia complex isolates from the onion rhizosphere and onion field soil. Appl Environ Microbiol 74:3121–3129. doi:10.1128/AEM.01941-07

    Article  CAS  Google Scholar 

  • Jolley KA, Chan MS, Maiden MC (2004) mlstdbNet–distributed multi-locus sequence typing (MLST) databases. BMC Bioinformatics 5:86. doi:10.1186/1471-2105-5-86

    Article  Google Scholar 

  • Lawongsa P, Boonkerd N, Wongkaew S, O’Gara F, Teaumroong N (2008) Molecular and phenotypic characterization of potential plant growth-promoting Pseudomonas from rice and maize rhizospheres. World J Microbiol Biotechnol 24:1877–1884. doi:10.1007/s11274-008-9685-7

    Article  Google Scholar 

  • Li B, Xie GL, Zhang JZ, Janssens D, Swings J (2006) Identification of the bacterial leaf spot pathogen of poinsettia in China. J Phytopathol 154:711–715. doi:10.1111/j.1439-0434.2006.01178.x

    Article  CAS  Google Scholar 

  • Li B, Xu LH, Lou MM, Li F, Zhang YD, Xie GL (2008) Isolation and characterization of antagonistic bacteria against bacterial leaf spot of Euphorbia pulcherrima. Lett Appl Microbiol 46:450–455. doi:10.1111/j.1472-765X.2008.02337.x

    Article  CAS  Google Scholar 

  • Li B, Yu RR, Yu SH, Qiu W, Fang Y, Xie GL (2009) First report on bacterial heart rot of garlic caused by Pseudomonas fluorescens in China. Plant Pathol J 25:91–94

    Article  Google Scholar 

  • Li B, Fang Y, Zhang GQ, Yu RR, Lou MM, Xie GL, Wang YL, Sun GC (2010) Molecular characterization of Burkholderia cepacia complex isolates causing bacterial fruit rot of apricot. Plant Pathol J 26:223–230

    Article  CAS  Google Scholar 

  • Ludovic V, Groleau MC, Dekimpe V, Deziel E (2007) Burkholderia diversity and versatility: an inventory of the extracellular products. J Microbiol Biotechnol 17:1407–1429

    Google Scholar 

  • Mahenthiralingam E, Bischof J, Byrne SK, Radomski C, Davies JE, Av-Gay Y, Vandamme P (2000) DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J Clin Microbiol 38:3165–3173

    CAS  Google Scholar 

  • Mahenthiralingam E, Baldwin A, Dowson CG (2008) Burkholderia cepacia complex bacteria: opportunistic pathogens with important natural biology. J Appl Microbiol 104:1539–1551. doi:10.1111/j.1365-2672.2007.03706.x

    Article  CAS  Google Scholar 

  • Padaria JC, Singh A (2009) Molecular characterization of soil bacteria antagonistic to Rhizoctonia solani, sheath blight of rice. J Environ Sci Heal B 44:397–402

    Article  CAS  Google Scholar 

  • Payne GW, Vandamme P, Morgan SH, LiPuma JJ, Coenye T, Weightman AJ, Hefin Jones T, Mahenthiralingam E (2005) Development of a recA gene-based identification approach for the entire Burkholderia genus. Appl Environ Microbiol 71:3917–3927. doi:10.1128/AEM.71.7.3917-3927.2005

    Article  CAS  Google Scholar 

  • Pengnoo A, Wiwattanapattapee R, Chumthong A, Kanjanamaneesathian M (2006) Bacterial antagonist as seed treatment to control leaf blight disease of bambara groundnut (Vigna subterranea). World J Microbiol Biotechnol 22:9–14. doi:10.1007/s11274-005-2820-9

    Article  Google Scholar 

  • Perin L, Martínez-Aguilar L, Castro-González R, Estrada-de los Santos P, Cabellos-Avelar T, Guedes HV, Reis VM, Caballero-Mellado J (2006) Diazotrophic Burkholderia species associated with field-grown maize and sugarcane. Appl Environ Microbiol 72:3103–3110. doi:10.1128/AEM.72.5.3103-3110.2006

    Article  CAS  Google Scholar 

  • Ren HY, Gu GY, Long JY, Yin Q, Wu TQ, Song T, Zhang SJ, Chen ZY, Dong HS (2006) Combinative effects of a bacterial type-III effector and a biocontrol bacterium on rice growth and disease resistance. J Biosci 31:617–627

    Article  CAS  Google Scholar 

  • Saikia R, Kumar R, Arora DK, Gogoi DK, Azad P (2006) Pseudomonas aeruginosa inducing rice resistance against Rhizoctonia solani: production of salicylic acid and peroxidases. Folia Microbiol 51:375–380

    Article  CAS  Google Scholar 

  • Schaad NW, Jones JB, Chun W (2001) Laboratory guide for identification of plant pathogenic bacteria, 3rd edn. American Phytopathological Society, Minnesota

    Google Scholar 

  • Sexton MM, Jones AL, Chaowagul W, Woods DE (1994) Purification and characterization of a protease from Pseudomonas pseudomallei. Can J Microbiol 40:903–910

    Article  CAS  Google Scholar 

  • Sokol PA, Ohman DE, Iglewski BH (1979) A more sensitive plate assay for detection of protease production by Pseudomonas aeruginosa. J Clin Microbiol 9:538–540

    CAS  Google Scholar 

  • Stanier RY, Palleroni NJ, Doudoroff M (1966) The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43:159–271. doi:10.1099/00221287-43-2-159

    CAS  Google Scholar 

  • Stead DE (1992) Grouping of plant-pathogenic bacteria and some other Pseudornonas spp. by using cellular fatty acid profiles. Int J Syst Bacteriol 42:281–295

    Article  CAS  Google Scholar 

  • Vanlaere E, LiPuma JJ, Baldwin A, Henry D, Brandt ED, Mahenthiralingam E, Speert DP, Dowson C, Vandamme P (2008) Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. Int J Syst Evol Microbiol 58:1580–1590. doi:10.1099/ijs.0.65634-0

    Article  CAS  Google Scholar 

  • Vanlaere E, Baldwin A, Gevers D, Henry D, Brandt ED, LiPuma JJ, Mahenthiralingam E, Speert DP, Dowson C, Vandamme P (2009) Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species: Burkholderia contaminans sp. Nov. and Burkholderia lata sp. Nov. Int J Syst Evol Microbiol 59:102–111. doi:10.1099/ijs.0.001123-0

    Article  CAS  Google Scholar 

  • Vermis K, Coenye T, Mahenthiralingam E, Nelis HJ, Vandamme P (2002) Evaluation of species-specific recA-based PCR tests for genomovar level identification within the Burkholderia cepacia complex. J Med Microbiol 51:937–940

    CAS  Google Scholar 

  • Wiwattanapatapee R, Chumthong A, Pengnoo A, Kanjanamaneesathian A (2007) Effervescent fast-disintegrating bacterial fortnulation for biological control of rice sheath blight. J Control Release 119:229–235. doi:10.1016/j.jconrel.2007.01.015

    Article  CAS  Google Scholar 

  • Yabuuchi E, Kosako Y, Oyaizu H, Yano I, Hotta H, Hashimoto Y, Ezaki T, Arakawa M (1992) Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol 36:1251–1275

    CAS  Google Scholar 

  • Yang DJ, Wang B, Wang JX, Chen Y, Zhou MG (2009) Activity and efficacy of Bacillus subtilis strain NJ-18 against rice sheath blight and Sclerotinia stem rot of rape. Biol Control 51:61–65. doi:10.1016/j.biocontrol.2009.05.021

    Article  Google Scholar 

  • Yu YM, Zhao BP, Gao HR, Wu HT, Yu M (2010) Restraining effect of biological controlling strain R13 on rice sheath blight. Heilongjiang Agricult Sci 1:3–4

    Google Scholar 

  • Zhang LX, Xie GL (2007) Diversity and distribution of Burkholderia cepacia complex in the rhizosphere of rice and maize. FEMS Microbiol Lett 266:231–235. doi:10.1111/j.1574-6968.2006.00530.x

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This project was supported by Zhejiang Provincial Natural Science Foundation of China (Y3090150), the Fundamental Research Funds for the Central Universities (KYJD09022), Zhejiang Provincial Project (2010R10091), the Agricultural Ministry of China (nyhyzx07-056; nyhyzx200803010) and Specialized Research Fund for the Doctoral Program of Higher Education (20090101120083).

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, China

    Bin Li, Bao-Ping Liu, Miao-Miao Lou, Guan-Lin Xie & Hong-Ye Li

  2. Zhejiang University of Technology, Hangzhou, 310032, China

    Rong-Rong Yu

  3. State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest and Disease Control, Institute of Plant Protection Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Yan-Li Wang & Guo-Chang Sun

Authors
  1. Bin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bao-Ping Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rong-Rong Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Miao-Miao Lou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yan-Li Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Guan-Lin Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hong-Ye Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Guo-Chang Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Bin Li, Guan-Lin Xie or Guo-Chang Sun.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, B., Liu, BP., Yu, RR. et al. Phenotypic and molecular characterization of rhizobacterium Burkholderia sp. strain R456 antagonistic to Rhizoctonia solani, sheath blight of rice. World J Microbiol Biotechnol 27, 2305–2313 (2011). https://doi.org/10.1007/s11274-011-0696-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-011-0696-4

Keywords

Search

Navigation