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The effect of Baccharis glutinosa extract on the growth of mycotoxigenic fungi and fumonisin B1 and aflatoxin B1 production

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Abstract

The aim of this study was to evaluate the effect of Baccharis glutinosa isolated extract on the growth of Aspergillus flavus and Aspergillus parasiticus, and their aflatoxin B1 production; and growth of Fusarium verticillioides, and their fumonisin B1 production. The three fungi were exposed to an antifungal fraction, designated as fraction F6-1, isolated from B. glutinosa by methanolic extraction followed by silica gel chromatography. The growth of the fungi was evaluated in kinetics of radial extension growth, kinetics of spores germination, length and diameter of hyphae, spores diameter, as well as in aflatoxin B1 and fumonisin B1 production. Fraction F6-1 caused radial growth inhibition of the three fungi mainly F. verticillioides. Spores germination of A. flavus and A. parasiticus was delayed in the early stage of the incubation time, although they completely germinated at 27 h. In contrast, spore germination of F. verticillioides was inhibited 87.7% up to 96 h. The lengths and diameters of hyphae, and spore diameters of the three fungi, were significantly smaller in comparison with those of the controls, and several morphological alterations were observed. Concerning aflatoxin B1 and fumonisin B1, fraction F6-1 did not show any inhibition effect at the concentration used. Fraction F6-1 was able to significantly inhibit the development of the three fungi, mainly F. verticillioides. The strong inhibitory effect of F6-1 on hyphae and spores suggests that it interacted with the fungi cell walls, which caused severe deformities. Nevertheless, this fraction was unable in inhibiting mycotoxin production from the three fungi at the concentration tested.

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Authors and Affiliations

  1. Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Unidad Regional Centro, Blvd. Luis Encinas y Rosales s/n. Col. Centro, P.O. Box 1658, 83000, Hermosillo, Sonora, México

    Ema Carina Rosas-Burgos, Mario Onofre Cortez-Rocha, Maribel Plascencia-Jatomea & Francisco Javier Cinco-Moroyoqui

  2. Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Apdo Postal 1658, 83000, Hermosillo, Sonora, México

    Ramón Enrique Robles-Zepeda

  3. Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de febrero No. 818 Sur, Apdo Postal 541, 85000, Ciudad Obregón, Sonora, México

    Ema Carina Rosas-Burgos, Jaime López-Cervantes, Dalia Isabel Sánchez-Machado & Fernando Lares-Villa

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  1. Ema Carina Rosas-Burgos
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  2. Mario Onofre Cortez-Rocha
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  3. Maribel Plascencia-Jatomea
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  4. Francisco Javier Cinco-Moroyoqui
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  6. Jaime López-Cervantes
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  7. Dalia Isabel Sánchez-Machado
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Correspondence to Ema Carina Rosas-Burgos.

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Rosas-Burgos, E.C., Cortez-Rocha, M.O., Plascencia-Jatomea, M. et al. The effect of Baccharis glutinosa extract on the growth of mycotoxigenic fungi and fumonisin B1 and aflatoxin B1 production. World J Microbiol Biotechnol 27, 1025–1033 (2011). https://doi.org/10.1007/s11274-010-0547-8

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  • DOI: https://doi.org/10.1007/s11274-010-0547-8

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