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Rubrivivaxin, a new cytotoxic and cyclooxygenase-I inhibitory metabolite from Rubrivivax benzoatilyticus JA2

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Abstract

A new phenol terpenoid ester metabolite was isolated from the culture supernatant of Rubrivivax benzoatilyticus JA2 when the organism grown on L-phenylalanine as sole source of nitrogen under photoheterotrophic condition. The compound was characterized by HPLC, LCMS, IR, NMR (1H, 13C) and Mass spectrometry, as a 3, 4-dihydroxybenzoyl-terpenoid ester [IUPAC: 3, 4-dihydroxy, 5-carboxy, 3-methylpentyl ester], which has a molecular mass (m/z) of 298 and we designated as rubrivivaxin. The significance of cyclooxygenase-I (COX-I) inhibitory, antimicrobial, cytotoxic activities against U937 (Human leukemic monocyte lymphoma) and Jurkat (T lymphocyte) cell lines conferred by rubrivivaxin.

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Acknowledgments

Dr. Ranjith Naik Kumavath acknowledges the UGC, Govt. of India for the award of Dr.D.S.Kothari-PDF. Financial support received from CSIR and infrastructural facilities under DST-FIST are acknowledged. The authors thank Prof. D. Basavaiah (LCMS, NMR, and IR); Dr. Venkateswa Rao, IICT, Hyderabad and Prof. P. Reddanna, University of Hyderabad, India (Cytotoxity studies and Cox- 1&2 inhibitory studies) are acknowledged for analytical support.

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Authors and Affiliations

  1. School of Biological Sciences, Nanyang Technological Sciences, Singapore, 637551, Republic of Singapore

    Ranjith N. Kumavath

  2. Bacterial Discovery and Metabolites Inventory Laboratory, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500 046, India

    Ranjith N. Kumavath & Ch. V. Ramana

  3. Bacterial Discovery Laboratory, Center for Environment, IST, JNT University, Kukatpally, Hyderabad, 500 085, India

    Ch. Sasikala

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  1. Ranjith N. Kumavath
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  2. Ch. V. Ramana
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  3. Ch. Sasikala
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Correspondence to Ranjith N. Kumavath.

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Kumavath, R.N., Ramana, C.V. & Sasikala, C. Rubrivivaxin, a new cytotoxic and cyclooxygenase-I inhibitory metabolite from Rubrivivax benzoatilyticus JA2. World J Microbiol Biotechnol 27, 11–16 (2011). https://doi.org/10.1007/s11274-010-0420-9

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  • DOI: https://doi.org/10.1007/s11274-010-0420-9

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