Abstract
Proteins from the extracellular medium of Aeropyrum pernix K1 were separated by two-dimensional electrophoresis and identified using mass spectrometry. Six different substrate-binding proteins (SBPs) from the ATP-binding cassette (ABC) transporter family were identified: (1) ABC transporter SBP (Q9YC61); (2) Branched-chain amino-acid ABC transporter, branched-chain amino-acid-binding protein (Q9YDJ6); (3) Oligopeptide ABC transporter, oligopeptide-binding protein (Q9YBL5); (4) Probable ABC transporter SBP (Q9Y9N4); (5) ABC transporter SBP (Q9YBG7); (6) ABC transporter SBP (Q9YFD7). Based on their orthology, division into the following classes was predicted: (1) multiple sugar-transport system SBPs; (2) peptide/nickel-transport system SBPs; and (3) branched-chain amino-acid-transport system SBPs. Further bioinformatic analyses showed that the identified SBPs differ in motif and in transmembrane-domain and signal-peptide organisation. Additionally, for all of these SBPs, sequence homology was found for archaeal proteins, and homologous proteins in bacteria were also found for the ABC transporter SBP Q9YBG7 and the ABC transporter SBP Q9YFD7. This is the first study, where different ABC SBPs from the extracellular medium of A. pernix have been identified using the combined methodology of two-dimensional electrophoresis and mass spectrometry.
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References
Aivaliotis M, Macek B, Gnad F, Reichelt P, Mann M, Oesterhelt D (2009) Ser/Thr/Try protein phosphorylation in the archaeon Halobacterium salinarum—a representative of the third domain of life. PLoS ONE 4:e4777
Albers SV, Driessen AJM (2002) Signal peptides of secreted proteins of the archaeon Sulfolobus solfataricus: a genomic survey. Arch Microbiol 177:209–216
Albers SV, Driessen AJM (2005) Analysis of ATPases of putative secretion operons in the thermoacidophilic archaeon Sulfolobus solfataricus. Microbiology 151:763–773
Albers SV, Elferink MG, Charlebois RL, Sensen CW, Driessen AJ, Konnings WN (1999) Glucose transport in the extremely thermoacidophilic Sulfolobus solfataricus involves a high-affinity membrane-integrated binding protein. J Bacteriol 181:4285–4291
Albers SV, Koning SM, Konings WN, Driessen AJ (2004) Insights into ABC transport in archaea. J Bioenerg Biomembr 36:5–15
Albers SV, Szabo Z, Driessen AJ (2006) Protein secretion in the archaea: multiple paths towards a unique cell surface. Nat Rev Microbiol 4:537–547
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal Biochem 72:248–254
Catara G, Ruggiero G, La Cara F, Digilio FA, Capasso A, Rossi M (2003) A novel extracellular subtilisin-like protease from the hyperthermophile Aeropyrum pernix K1: biochemical properties, cloning, and expression. Extremophiles 7:391–399
Detmers FJ, Lanfermeijer FC, Poolman B (2001) Peptides and ATP binding cassette peptide transporters. Res Microbiol 152:245–258
Eichler J, Adams MWW (2005) Posttranslational protein modification in Archaea. Microbiol Mol Biol Rev 69:393–425
Elferink MG, Albers SV, Koning WN, Driessen AJ (2001) Sugar transport in Sulfolobus solfataricus is mediated by two families of binding protein-dependent ABC transporters. Mol Microbiol 39:1494–1503
Görg A (1991) Two-dimensional electrophoresis. Nature 349:545–546
Horlacher R, Xavier KB, Santos H, Di Ruggiero J, Kossmann M, Boos W (1998) Archaeal binding protein-dependent ABC transporter: molecular and biochemical analysis of the trehalose/maltose transport system of the hyperthermophilic archaeon Thermococcus litoralis. J Bacteriol 180:680–689
Huber H, Stetter KO (1998) Hyperthermophiles and their possible potential in biotechnology. J Biotechnol 64:39–52
Kim KW, Lee SB (2003) Growth of the hyperthermophilic marine archaeon Aeropyrum pernix in a defined medium. J Biosci Bioeng 95:618–622
Milek I, Cigić B, Skrt M, Kaletunc G, Poklar Ulrih N (2005) Optimisation of growth for the hyperthermophilic archaeon Aeropyrum pernix on a small-batch scale. Can J Microbiol 51:805–809
Nanavati DA, Thirangoon K, Noll KM (2006) Several archaeal homologues of putative oligopeptide-binding proteins encoded by Thermotoga maritima bind sugars. Appl Environ Microbiol 72:1336–1345
Nelson KE, Eisen JA, Fraser CM (2001) Genome of Thermotoga maritima MSB8. Methods Enzymol 330:169–180
Nesbo CL, L’Haridon S, Stetter KO, Doolittle WF (2001) Phylogenetic analyses of two “archaeal” genes in Thermotoga maritima reveal multiple transfers between archaea and bacteria. Mol Biol Evol 18:362–375
Nesbo CL, Nelson KE, Doolittle WF (2002) Suppressive subtractive hybridisation detects extensive genomic diversity in Thermotoga maritima. J Bacteriol 184:4475–4488
Palmieri G, Casbarra A, Marino G, Catara G, Ruggiero G, Capasso A, Rossi M (2005) High cleavage specificity of a subtilisin-like protease from a hyperthermophilic archaeon under extreme conditions. Enzyme Microb Technol 37:745–749
Palmieri G, Casbarra A, Fiume I, Catara G, Capasso A, Marino G, Onesti S, Rossi M (2006) Identification of the first archaeal oligopeptide-binding protein from the hyperthermophile Aeropyrum pernix. Extremophiles 10:393–402
Sako Y, Nomura N, Uchida A, Ishida Y, Morii H, Koga Y, Hoaki T, Maruyama T (1996) Aeropyrum pernix gen. nov., sp. nov., a novel aerobic hyperthermophilic archaeon growing at temperatures up to 100°C. Int J Syst Bacteriol 46:1070–1077
Stetter K (1996) Hyperthermophilic prokaryotes. FEMS Microbiol Rev 18:149–158
Szabo ZA, Stahl O, Albers SV, Kissinger JC, Driessen AJM, Pohlschroder M (2007) Identification of diverse archaeal proteins with class III signal peptides cleaved by distinct archaeal prepilin peptidases. J Bacteriol 189:772–778
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This study was supported by the Ministry of Higher Education, Science and Technology of the Republic of Slovenia (Research programme: Biochemical and Biophysical Characterisation of Natural Compounds: P4-0121). The Aberdeen Proteome Facility is funded jointly by the Scottish Higher Education Funding Council (SHEFC), the Biotechnology and Biological Sciences Research Council (BBSRC) and the University of Aberdeen.
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Jamnik, P., Meglen, M., Raspor, P. et al. Identification of various substrate-binding proteins of the hyperthermophylic archaeon Aeropyrum pernix K1. World J Microbiol Biotechnol 26, 1579–1586 (2010). https://doi.org/10.1007/s11274-010-0333-7
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DOI: https://doi.org/10.1007/s11274-010-0333-7