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Actinomycetes and fungi isolated from plant-parasitic nematode infested soils: screening of the effective biocontrol potential, indole-3-acetic acid and siderophore production

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Abstract

Root-knot nematodes are serious pathogens that severe damage to major crops. They damage plant root system that caused significant yield losses. Moreover, the predisposition of nematode-infected plants is secondary infection from fungal plant pathogen that additional adverse effects on plant growth. Our target is to find the antagonist for control nematode, and secondary infection agents and stimulate plant growth. Twenty-three plant-parasitic nematode infested soils were taken from some provinces in the northern and center of Thailand and actinomycetes and fungi were isolated. Eighty-three isolates belong to actinomycete and 67 isolates were fungi. The predominant actinomycete taxa was Streptomyces (97.6%). The predominant fungal taxa were Penicillium (37.3%) and Fusarium (32.8%). All actinomycete and fungal isolates were subjected for primary screening in vitro for their effects on egg hatching and juvenile mortality of Meloidogyne incognita. Secondary screening was evaluated for antagonist effect on plant pathogenic fungi collected from nematode-infected plant, plant growth hormone (indole-3-acetic acid; IAA) and siderophore production. From primary screening, 7 actinomycete and 10 fungal isolates reduced egg hatching and kill juveniles of M. incognita after 7 days incubation. In secondary screening, 10 nematophagous microbes produced IAA and 9 isolates produced hydroxamate siderophore. Streptomyces sp. CMU-MH021 was selected as a potential biocontrol agent. It reduced egg hatching rate to 33.1% and increased juvenile mortality rate to 82% as contrasted to the control of 79.6 and 3.6%, respectively. This strain had high activity to against tested fungi and high ability on IAA (28.5 μg ml−1) and siderophore (26.0 μg ml−1) production.

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Abbreviations

Ca:

Circa

CAS:

Chrome azurol S

RNKs:

Root-knot nematodes

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Acknowledgments

This research was supported by The Royal Golden Jubilee Ph. D. Program (PHD/0064/2549), The Commission on Higher Education, Thailand on Target Research Initiative Program and Graduate School, Chiang Mai University. We are grateful to Professor John Peberdy, University of Nottingham and Arjan Morakot Sukchotiratana, Chiang Mai University for improving the English text.

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Authors and Affiliations

  1. Biotechnology Program, Graduate School, Chiang Mai University, Chiang Mai, 50200, Thailand

    Pornthip Ruanpanun & Saisamorn Lumyong

  2. Department of Agriculture, Nematology Section, Plant Protection Research and Development Office, Bangkok, 10900, Thailand

    Nuchanart Tangchitsomkid

  3. School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    Kevin D. Hyde

  4. Microbiology Division, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand

    Pornthip Ruanpanun & Saisamorn Lumyong

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  1. Pornthip Ruanpanun
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Correspondence to Saisamorn Lumyong.

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Ruanpanun, P., Tangchitsomkid, N., Hyde, K.D. et al. Actinomycetes and fungi isolated from plant-parasitic nematode infested soils: screening of the effective biocontrol potential, indole-3-acetic acid and siderophore production. World J Microbiol Biotechnol 26, 1569–1578 (2010). https://doi.org/10.1007/s11274-010-0332-8

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