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Rhizobacterial potential to alter auxin content and growth of Vigna radiata (L.)

Abstract

Potential of non-symbiotic plant growth promoting rhizobacteria (PGPR) to influence the endogenous indole-3-acetic acid (IAA) content and growth of Vigna radiata (L.) was evaluated. The bacterial strains used belonged to Pseudomonas, Escherichia, Micrococcus and Staphylococcus genera. All strains were able to produce IAA (1.16–8.22 μg ml−1) in the presence of 1,000 μg ml−1 of l-tryptophan as revealed by gas chromatography and mass spectrometric (GC–MS) analysis. However, strains exhibited variable results for other growth promoting traits such as phosphate solubilization and siderophore or hydrogen cyanide production. Bacterial IAA production showed significant positive correlation with endogenous IAA content of roots (r = 0.969; P = 0.01) and leaves (r = 0.905; P = 0.01) under axenic conditions. Bacterization of V. radiata seeds significantly enhanced shoot length (up to 48.10%) and shoot fresh biomass (up to 43.80%) under fully axenic conditions. Bacterial strains applied under wire-house conditions also improved shoot length, number of pods, and grain weight up to 58, 65, and 17.15% respectively, over control. Hence, free living (non-symbiotic) PGPR have the ability to influence endogenous IAA content and growth of leguminous plants.

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Acknowledgments

We thank Dr. Karin Ljung, Umeå Plant Science Centre (UPSC), Swedish University of Agricultural Sciences, Umeå, Sweden, for GC–MS facilities.

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Correspondence to Shahida Hasnain.

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Ali, B., Sabri, A.N. & Hasnain, S. Rhizobacterial potential to alter auxin content and growth of Vigna radiata (L.). World J Microbiol Biotechnol 26, 1379–1384 (2010). https://doi.org/10.1007/s11274-010-0310-1

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Keywords

  • Non-symbiotic PGPR
  • Endogenous IAA
  • Growth promoting traits
  • l-Tryptophan
  • Axenic conditions