Abstract
The various marine cyanobacterial strains tested showed wide variation in growth patterns and decolourization patterns of the lignin model polymeric dye Poly R-478. The study revealed the presence of laccases (LACs) and polyphenol oxidases (PPOs) in marine cyanobacteria. All the ten tested strains were found to possess constitutive PPOs, whereas only four strains showed the presence of constitutive laccases. Within 7 days of incubation the highest percentage of decolourization was shown by Phormidium valderianum BDU140441 (65%), and Oscillatoria chlorina BDU 140691 (12%) showed the least. Isoforms of LACs were found to be induced by the laccase elicitors veratryl aldehyde, caffeic acid, guaiacol and tannic acid. Cyanobacterial strains that possess both LACs and PPOs were relatively more efficient in decolourizing the dye. Altering the concentrations of nitrogen, phosphorus, potassium and sulphur from the basal medium influenced the efficiency of dye decolourization.
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The authors thank the Department of Biotechnology (DBT), Government of India, for financing the facility and fellowship to P Swaminathan.
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Palanisami, S., Saha, S.K. & Lakshmanan, U. Laccase and polyphenol oxidase activities of marine cyanobacteria: a study with Poly R-478 decolourization. World J Microbiol Biotechnol 26, 63–69 (2010). https://doi.org/10.1007/s11274-009-0143-y
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DOI: https://doi.org/10.1007/s11274-009-0143-y