Abstract
Two distinct nitrifying bacterial consortia, namely an ammonia oxidizing non-penaeid culture (AMONPCU-1) and an ammonia oxidizing penaeid culture (AMOPCU-1), have been mass produced in a nitrifying bacterial consortia production unit (NBCPU). The consortia, maintained at 4°C were activated and cultured in a 2 l fermentor initially. At this stage the net biomass (0.105 and 0.112 g/l), maximum specific growth rate (0.112 and 0.105/h) and yield coefficients (1.315 and 2.08) were calculated respectively, for AMONPCU-1 and AMOPCU-1 on attaining stationary growth phase. Subsequently on mass production in a 200 l NBCPU under optimized culture conditions, the total amounts of NH4 +–N removed by AMONPCU-1 and AMOPCU-1 were 1.948 and 1.242 g/l within 160 and 270 days, respectively. Total alkalinity reduction of 11.7–14.4 and 7.5–9.1 g/l were observed which led to the consumption of 78 and 62 g Na2CO3. The yield coefficient and biomass of AMONPCU-1 were 0.67 and 125.3 g/l and those of AMOPCU-1 were 1.23 and 165 g/l. The higher yield coefficient and growth rate of AMOPCU-1 suggest better energy conversion efficiency and higher CO2 fixation potential. Both of the consortia were dominated by Nitrosomonas-like organisms. The consortia may find application in the establishment of nitrification within marine and brackish water culture systems.
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Acknowledgements
This work was carried out with the financial assistance from Department of Biotechnology, Government of India under project no. BT/DRI 794/AAQ/03/092/99. Acknowledge Dr. Valsamma Joseph, Lecturer, NCAAH, CUSAT for analysis of the data and Prof. Robert H. Reed, Central Queensland University, Australia for language editing.
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Rejish Kumar, V.J., Achuthan, C., Manju, N.J. et al. Mass production of nitrifying bacterial consortia for the rapid establishment of nitrification in saline recirculating aquaculture systems. World J Microbiol Biotechnol 25, 407–414 (2009). https://doi.org/10.1007/s11274-008-9905-1
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DOI: https://doi.org/10.1007/s11274-008-9905-1