Abstract
Lipase-catalyzed transesterification of soybean oil and methanol for biodiesel production in tert-amyl alcohol was investigated. The effects of different organic medium, molar ratio of substrate, reaction temperature, agitation speed, lipase dosage and water content on the total conversion were systematically analyzed. Under the optimal conditions identified (6 mL tert-amyl alcohol, three molar ratio of methanol to oil, 2% Novozym 435 lipase based on the soybean oil weight, temperature 40°C, 2% water content based on soybean oil weight, 150 rpm and 15 h), the highest biodiesel conversion yield of 97% was obtained. With tert-amyl alcohol as the reaction medium, the negative effects caused by excessive molar ratio of methanol to oil and the by-product glycerol could be reduced. Furthermore, there was no evident loss in the lipase activity even after being repeatedly used for more than 150 runs.
Similar content being viewed by others
References
Adlercreutz P (1999) Biocatalysis in non-conventional media. In: Straathof JJ (ed) Applied biocatalysis. Harwood Academic Publishers, Amsterdam
Carrea G, Ottolina G, Riva S (1995) Role of solvent in the control of enzyme selectivity in organic media. Trends Biotechnol 13:63–70. doi:10.1016/S0167-7799(00)88907-6
Catoni E, Cernia E, Palocci C (1996) Different aspects of ‘solvent engineering’ in lipase biocatalysed esterifications. J Mol Catal B Enzym 105:79–86
Claude S (1999) Research of new outlets for glycerol-recent developments in France. Fett/Lipid 101:101–104
Dossat V, Combes D, Marty A (1999) Continuous enzymatic transesterification of high oleic sunflower oil in a packed bed reactor: influence of the production of glycerol. Enzyme Microb Technol 25:194–200. doi:10.1016/S0141-0229(99)00026-5
Du W, Liu DH, Li LL et al (2007) Methanism exploration during lipase-mediated methanolysis of Renewable Oils for Biodiesel production in a tert-butanol system. Biotechnol Prog 23:1087–1090
Fernando S, Karra P, Hernandez R et al (2007) Effect of incompletely converted soybean oil on biodiesel quality. Energy 32:844–851. doi:10.1016/j.energy.2006.06.019
Fukuda H, Kondo A, Noda H (2001) Biodiesel fuel production by transesterification of oils. J Biosci Bioeng 92:405–416. doi:10.1263/jbb.92.405
Kaieda M, Samukawa T, Kondo A et al (2001) Effect of methanol and water contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a solvent-free system. J Biosci Bioeng 91:12–15. doi:10.1263/jbb.91.12
Krisnangkura K, Yimsuwan T, Pairintra R (2006) An empirical approach in predicting biodiesel viscosity at various temperatures. Fuel 85:107–113. doi:10.1016/j.fuel.2005.05.010
Kusdiana D, Saka S (2004) Effects of water on biodiesel fuel production by supercritical methanol treatment. Bioresour Technol 91:289–295. doi:10.1016/S0960-8524(03)00201-3
Li LL, Du W, Liu DH et al (2006) Lipase-catalyzed transesterification of rapeseed oils for biodiesel production with a novel organic solvent as the reaction medium. J Mol Catal B Enzym 43:58–62. doi:10.1016/j.molcatb.2006.06.012
Lu JK, Nie KL, Xie F et al (2007) Enzymatic synthesis of fatty acid methyl esters from lard with immobilized Candida sp. 99–125. Process Biochem 42:1367–1370. doi:10.1016/j.procbio.2007.06.004
Nie KL, Xie F, Wang F et al (2006) Lipase catalyzed methanolysis to produce biodiesel: optimization of the biodiesel production. J Mol Catal B Enzym 43:132–137. doi:10.1016/j.molcatb.2006.07.016
Shah S, Gupta MN (2007) Lipase catalyzed preparation of biodiesel from jatropha oil in a solvent free system. Process Biochem 42:409–414. doi:10.1016/j.procbio.2006.09.024
Shah S, Sharma A, Gupta MN (2006) Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a protein feeder. Anal Biochem 351:207–213. doi:10.1016/j.ab.2006.01.028
Shieh CJ, Liao HF, Lee CC (2003) Optimization of lipase-catalyzed biodiesel by response surface methodology. Bioresour Technol 88:103–106. doi:10.1016/S0960-8524(02)00292-4
Shimada Y, Watannabe Y, Samukawa T et al (1999) Conversion of vegetable oil to biodiesel using immobilized Candida antarctica lipase. J Am Oil Chem Soc 76:789–793. doi:10.1016/S1381-1177(02)00020-6
Shimada Y, Watanabe Y, Sughara A et al (2002) Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil process. J Mol Catal B Enzym 17:133–142. doi:10.1016/S1381-1177(02)00020-6
Soumanou MM, Bornscheuer UT (2003) Improved in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil. Enzyme Microb Technol 33:97–103. doi:10.1016/S0141-0229(03)00090-5
Twu YK, Shih IL YENYH et al (2005) Optimization of lipase catalyzed synthesis of octyl hydroxyphenylpropionate by response surface methodology. J Agric Food Chem 53:1012–1016. doi:10.1021/jf048732o
Vali SK, Sheng HY, Ju TH (2003) An efficient method for the purification of arachidonic acid from fungal single-cell oil (ARASCO). J Am Oil Soc 80:725–730. doi:10.1007/s11746-003-0764-3
Vanessa Lara P, Park EY (2004) Potential application of waste activated bleaching earch on the production of fatty acid alkyl esters using candida cylindracea lipase in organic solvent system. Enzyme Microb Technol 34:270–277. doi:10.1016/j.enzmictec.2003.10.015
Warabi Y, Kusdiana D, Saka S (2004) Reactivity of triglycerides and fatty acids of rapeseed oil in supercritical alcohols. Bioresour Technol 91:283–287. doi:10.1016/S0960-8524(03)00202-5
Xu YY, Du W, Liu DH et al (2003) A novel enzymatic route for biodiesel production from renewable oils in a solvent-free medium. Biotechnol Lett 25:1239–1241. doi:10.1023/A:1025065209983
Acknowledgments
This work was financially supported by the National Nature Science Foundation of Chinese (No. 50773009); the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0526); Biomedical Textile Materials “111 Project” from Ministry of Education of P.R. China (No.B07024)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zheng, Y., Quan, J., Ning, X. et al. Lipase-catalyzed transesterification of soybean oil for biodiesel production in tert-amyl alcohol. World J Microbiol Biotechnol 25, 41–46 (2009). https://doi.org/10.1007/s11274-008-9858-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-008-9858-4