Skip to main content
SpringerLink
Log in

Effect of additives on the esterification activity of immobilized Candida antarctica lipase

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

In this work, the stabilizing effect of bovine serum albumin (BSA), peptone (PEP), and polyethylene glycol (PEG) during immobilization of Candida antarctica lipase on activated carbon was investigated. The influence of enzyme concentration and type of additive, added during the immobilization procedure, was studied using a 22 factorial central composite design. The goal was to maximize the synthetic activity of butyl butyrate, using butyric acid and butanol as substrate in n-heptane. An increase of 31–58% in the esterification activity was obtained when enzyme concentration on the supernatant was enhanced from 86.50 U m L−1 to 226.80 U mL−1. An enhancement in esterification activity of 38–68.95% was observed, depending on the initial enzyme concentration, when PEP was used instead of BSA. No significant increase in the esterification activity was observed when PEP was replaced by PEG. However, thermal stability tests at 50 °C showed that PEG had a higher stabilizing effect.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Adriano WS, Veredas V, Santana CC, Gonçalves LRB (2005) Adsorption of amoxicillin on chitosan beads: kinetics, equilibrium and validation of finite bath models. Biochem Eng J 27:132–137

    Article  CAS  Google Scholar 

  • Arroyo M, Montero JMS, Sinisterra JV (1999) Thermal stabilization of immobilized lipase B from Candida antarctica on different supports: effect of water activity on enzymatic activity in organic media. Enzyme Microb Technol 24:3–12

    Article  CAS  Google Scholar 

  • Barros Neto BN, Scarminio IP, Bruns RE (2001) Planejamento e otimização de experimentos. 404 pp, ISBN 8526806661

  • Bastida A, Sabuquillo P, Armisen P, Lafuente RF, Huguet J, Guisan JM (1998) A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports. Biotechnol Bioeng 58:486–493

    Article  CAS  Google Scholar 

  • Blanco RM, Terreros P, Pérez MF, Otero C, González G (2004) Functionalization of mesoporous silica for lipase immobilization characterization of the support and the catalysts. J Mol Catal B: Enzym 30:83–93

    Article  CAS  Google Scholar 

  • Bornscheuer UT, Bessler C, Srinivas R, Krishna SH (2002) Optimizing lipases and related enzymes for efficient application. Trends Biotechnol 20:433–437

    Article  CAS  Google Scholar 

  • Bosley JA (1991) Supported enzymes. Patent application EQP 424130 A1

  • Fernandez-Lorente G, Fernandez-Lafuente R, Palomo JM, Mateo C, Bastida A, Coca J, Haramboure T, Hernandez-Justiz O, Terreni M, Guisán JM (2001) Biocatalyst engineering exerts a dramatic effect on selectivity of hydrolysis catalyzed by immobilized lipases in aqueous medium. J Mol Catal B: Enzym 11:649–656

    Article  CAS  Google Scholar 

  • Garrido-Lopez A, Tena MT (2005) Experimental design approach for the optimisation of pressurised fluid extraction of additives from polyethylene films. J Chromatogr A 1099:75–83

    Article  CAS  Google Scholar 

  • Gitlesen T, Bauer M, Adlercreutz PA (1997) Adsorption of lipase on polypropylene powder. Biochim Biophys Acta 1345:188–196

    CAS  Google Scholar 

  • Hung TC, Giridhar R, Chiou SH, Wu WT (2003) Binary immobilization of Candida rugosa lipase on chitosan. J Mol Catal B: Enzym 26:69–78

    Article  CAS  Google Scholar 

  • Kiran KR, Krishna SH, Babu CVS, Karant NG, Divakar S (2000) An esterification method for determination of lipase activity. Biotechnol Lett 22:1511–1514

    Article  CAS  Google Scholar 

  • Martinelle M, Holmquist M, Hult K (1995) On the interfacial activation of Candida antarctica lipase-A and lipase-B as compared with Humicola lanuginosa lipase. Biochim Biophys Acta 1258:272–276

    Google Scholar 

  • Montgomery DC, Runger GC (2003) Applied statistics and probability for engineers. John Wiley & Sons Inc., 720 pp, ISBN 978-0-471-20454-1

  • Palomo JM, Munõz G, Lorente GF, Mateo C, Lafuente RF, Guisán JM, (2002) Interfacial adsorption of lipases on very hydriphobic support (octadecyl-sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases. J Mol Catal B: Enzym 19:279–286

    Article  Google Scholar 

  • Reetz M, Zonta A, Simpelkamp J (1996) Efficient immobilization of lipases by entrapment in hydrophobic sol-gel materials. Biotechnol Bioeng 49:527–534

    Article  CAS  Google Scholar 

  • Rocha JMS, Gil MH, Garcia FAP (1998) Effects of additives on the activity of a covalently immobilised lipase in organic media. J Biotechnol 66:61–67

    Article  CAS  Google Scholar 

  • Rodrigues DS, Cavalcante GP, Ferreira ALO, Goncalves LRB (2008) Immobilization of Candida antarctica lipase type B by adsorption on activated carbon. Chem Biochem Eng Q (in press)

  • Soares CMF, Santana MHA, Zanin GM, Castro HF (2003a) Covalent coupling method for lipase immobilization on controlled pore silica in the presence of nonenzymatic proteins. Biotechnol Progr 19:803–807

    Article  CAS  Google Scholar 

  • Soares CMF, Santana MHA, Zanin GM, Castro HF (2003b) Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico. Química Nova 26:832–838

    Article  CAS  Google Scholar 

  • Soares CMF, Castro HF, Santana MHA, Zanin GM (2002) Intensification of lipase performance for long-term operation by immobilization on controlled pore silica in presence of polyethylene glycol. Appl Biochem Biotechnol 98:863–874

    Article  Google Scholar 

  • Soares CMF, Castro HF, Santana MHA, Zanin GM (2001) Selection of stabilizing additive for lipase immobilization on controlled pore silica by factorial design. Appl Biochem Biotechnol 91:703–718

    Article  Google Scholar 

  • Squire PG, Moser P, O’Konski CT (1968) The hydrodynamic properties of bovine serum albumin monomer and dimer. Biochemistry 7:4261–4272

    Article  CAS  Google Scholar 

  • Stark MB, Holmberg K (1989) Covalent immobilization of lipase in organic solvents. Biotechnol Bioeng 34:942–950

    Article  CAS  Google Scholar 

  • Triantaflyllou AO, Wehtje E, Adlercreutz P, Mattiasson B (1995) Effects of sorbitol addition on the action of free and immobilized hydrolytic enzymes in organic media. Biotechnol Bioeng 45:406–414

    Article  Google Scholar 

  • Uppenberg J, Hansen MT, Patkar S, Jones TA (1994) The sequence, crystal-structure determination and refinement of 2 crystal forms of lipase-B from Candida-antarctica. Structure 2:293–308

    Article  CAS  Google Scholar 

  • Wehtje E, Adlercreutz P, Mattiasson B (1993) Improved activity retention of enzymes deposited on solid supports. Biotechnol Bioeng 41:171–178

    Article  CAS  Google Scholar 

  • Xu HX, Li MQ, He BL (1995) Immobilization of Candida-cylindracea lipase on methyl acrylate-divinyl benzene copolymer and its derivatives. Enzyme Microb Technol 17:194–199

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the Brazilian research-funding agencies Finep, CNPq and CAPES (Federal) and FUNCAP (State of Ceará).

Author information

Authors and Affiliations

  1. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, Fortaleza, CE, CEP: 60455-760, Brazil

    Dasciana S. Rodrigues, George P. Cavalcante, Andrea L. O. Ferreira & Luciana R. B. Gonçalves

  2. Tecnologias Bioenergéticas – TECBIO, R. Rômulo Proença, S/N – Campus do Pici, Fortaleza, CE, 60455-760, Brazil

    Giovanilton F. Silva

Authors
  1. Dasciana S. Rodrigues
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. George P. Cavalcante
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Giovanilton F. Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Andrea L. O. Ferreira
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Luciana R. B. Gonçalves
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Luciana R. B. Gonçalves.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rodrigues, D.S., Cavalcante, G.P., Silva, G.F. et al. Effect of additives on the esterification activity of immobilized Candida antarctica lipase. World J Microbiol Biotechnol 24, 833–839 (2008). https://doi.org/10.1007/s11274-007-9548-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-007-9548-7

Keywords

Search

Navigation