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Improvement of echinacoside and acteoside production by two-stage elicitation in cell suspension culture of Cistanche deserticola

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Abstract

At appropriate concentrations, polyamines promoted the callus growth and echinacoside content of Cistanche deserticola while Ag+ increased the content of echinacoside and acteoside. In a 20-day culture period, when putrescine (25 μM) and Ag+ (10 μM) were added on day 8 and day 16, respectively, the echinacoside production (1.7 g l−1) and acteoside production (0.4 g l−1) reached the maximum, which were 1.4-fold and 1.5-fold of those in single putrescine treatment, 1.6-fold and 1.4-fold of those in single Ag+ treatment, respectively. Exogenous putrescine enhanced cell viability and antioxidant enzyme activity markedly, so increased the final biomass. Ag+ addition increased H2O2 content and phenylalanine ammonia-lyase activity significantly which led to higher echinacoside and acteoside contents.

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Abbreviations

APOX:

Ascorbate peroxidase

CAT:

Catalase

H2O2 :

Hydrogen peroxide

PAL:

Phenylalanine ammonia lyase

PeGs:

Phenylethanoid glycosides

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

References

  • Alcázar R, Marco F, Cuevas JC, Patron M, Ferrando A, Carrasco P, Tiburcio AF, Altabella T (2006) Involvement of polyamines in plant response to abiotic stress. Biotechnol Lett 28:1867–1876

    Article  Google Scholar 

  • Bae KH, Choi YE, Shin CG, Kim YY, Kim YS (2006) Enhanced ginsenoside productivity by combination of ethephon and methyl jasmoante in ginseng (Panax ginseng C.A. Meyer) adventitious root cultures. Biotechnol Lett 28:1163–1166

    Article  CAS  Google Scholar 

  • Bais HP, Ravishankar GA (2002) Role of polyamines in the ontogeny of plants and their biotechnological applications. Plant Cell Tissue Organ Cult 69:1–34

    Article  CAS  Google Scholar 

  • Cheng XY, Guo B, Zhou HY, Ni W, Liu CZ (2005) Repeated elicitation enhances phenylethanoid glycosides accumulation in cell suspension cultures of Cistanche deserticola. Biochem Eng J 24:203–207

    Article  CAS  Google Scholar 

  • Cheng XY, Zhou HY, Cui X, Ni W, Liu CZ (2006) Improvement of phenylethanoid glycosides biosynthesis in Cistanche deserticola cell suspension cultures by chitosan elicitor. J Biotechnol 121:253–260

    Article  CAS  Google Scholar 

  • Cona A, Rea G, Angelini R, Federico R, Tavladoraki P (2006) Functions of amine oxidases in plant development and defence. Trends Plant Sci 11:80–88

    Article  CAS  Google Scholar 

  • Desikan R, Reynolds A, Hancock JT, Neill SJ (1998) Harpin and hydrogen peroxide both initiate programmed cell death but have differential effects on defense gene expression in Arabidopsis suspension cultures. Biochem J 330:115–120

    CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  CAS  Google Scholar 

  • Jebara S, Jebara M, Limam F, Aouani ME (2005) Changes in ascorbate peroxidase, catalase, guaiacol peroxidase and superoxide dismutase activities in common bean (Phaseolus vulgaris) nodules under salt stress. J Plant Physiol 162:929–936

    Article  CAS  Google Scholar 

  • Khosroushahi AY, Valizadeh M, Ghasempour A, Khosrowshahli M, Naghdibadi H, Dadpour MR, Omid Y (2006) Improved taxol production by combination of inducing factors in suspension cell culture of Taxus baccata. Cell Biol Int 30:262–269

    Article  CAS  Google Scholar 

  • Koukol J, Conn EE (1961) The metabolism of aromatic and properties of the phenylalanine deaminase of Hordeum vulgare. J Biol Chem 236:2692–2698

    CAS  Google Scholar 

  • Lu CT, Mei XG (2003) Improvement of phenylethanoid glycosides production by a fungal elicitor in cell suspension culture of Cistanche deserticola. Biotechnol Lett 25:1437–1439

    Article  CAS  Google Scholar 

  • Ouyang J, Wang XD, Zhao B, Yuan XF, Wang YC (2003a) Formation of phenylethanoid glycosides by Cistanche deserticola callus grown on solid media. Biotechnol Lett 25:223–225

    Article  CAS  Google Scholar 

  • Ouyang J, Wang XD, Zhao B, Yuan XF, Wang YC (2003b) Effects of rare earth elements on the growth of Cistanche deserticola cells and the production of phenylethanoid glycosides. J Biotechnol 102:129–134

    Article  CAS  Google Scholar 

  • Papadakis AK, Roubelakis-Angelakis KA (2005) Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide. Planta 220:826–837

    Article  CAS  Google Scholar 

  • Song ZH, Lei L, Tu PF (2003) Advances in research of pharmacological activity in plants of Cistanche Hoffing et Link. Chin Tradit Herbal Drugs 34:16–18

    Google Scholar 

  • Tang W, Newton RJ (2005) Polyamines reduce salt-induced oxidative damage by increasing the activities of antioxidant enzymes and decreasing lipid peroxidation in Virginia pine. Plant Growth Regul 46:31–43

    Article  CAS  Google Scholar 

  • Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated been plants protective role of exogenous polyamines. Plant Sci 151:59–66

    Article  CAS  Google Scholar 

  • Verleysen H, Samyn G, Van Bockstaele E, Debergh P (2004) Evaluation of analytical techniques to predict viability after cryopreservation. Plant Cell Tissue Organ Cult 77:11–21

    Article  CAS  Google Scholar 

  • Xu MJ, Dong JF (2005) O2 − from elicitor-induced oxidative burst is necessary for triggering phenylalanine ammonia-lyase activation and catharanthine synthesis in Catharanthus roseus cell cultures. Enzyme Microb Technol 36:280–284

    Article  CAS  Google Scholar 

  • Xu LS, Xue XF, Fu CX, Jin ZP, Chen YQ, Zhao DX (2005) Effects of methyl jasmonate and salicylic acid on phenylethanoid glycosides synthesis in suspension cultures of Cistanche deserticola. Chin J Biotechnol 21:402–406

    CAS  Google Scholar 

  • Yuan YJ, Li C, Hu ZD, Wu JC (2001) Signal transduction pathway for oxidative burst and taxol production in suspension cultures of Taxus chinensis var. mairei induced by oligosaccharide from Fusarium oxysprum. Enzyme Microb Technol 29:372–379

    Article  CAS  Google Scholar 

  • Yuan YJ, Wei ZJ, Miao ZQ, Wu JC (2002) Acting paths of elicitors on Taxol biosynthesis pathway and their synergistic effect. Biochem Eng J 10:77–83

    Article  CAS  Google Scholar 

  • Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, P. R. China

    Wen-Hao Chen, Chun-Ming Xu, Jian-Li Zeng, Bing Zhao, Xiao-Dong Wang & Yu-Chun Wang

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, P. R. China

    Wen-Hao Chen, Chun-Ming Xu & Jian-Li Zeng

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  1. Wen-Hao Chen
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  2. Chun-Ming Xu
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  3. Jian-Li Zeng
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  4. Bing Zhao
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  5. Xiao-Dong Wang
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  6. Yu-Chun Wang
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Correspondence to Yu-Chun Wang.

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Chen, WH., Xu, CM., Zeng, JL. et al. Improvement of echinacoside and acteoside production by two-stage elicitation in cell suspension culture of Cistanche deserticola . World J Microbiol Biotechnol 23, 1451–1458 (2007). https://doi.org/10.1007/s11274-007-9389-4

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  • DOI: https://doi.org/10.1007/s11274-007-9389-4

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