Abstract
Bioaerosols in commercial livestock barns threaten the health of animals and humans. To better understand microbial dynamics in the layer house, we characterized and compared the size-segregated bacterial community of the manure cleaning process in a typical commercial laying hen house equipped with an H-type manure removal belt and negative pressure tunnel ventilation system in Ningbo, China by using full-length 16S rRNA gene sequences. The third-generation single-molecule real-time sequencing (SMRT Cell) based on the Pacific BioSciences (PacBio) RS II platform was performed for capturing sequence information of the microbial aerosol samples. The results showed that the concentrations of total airborne bacteria in the layer house were in the range of 0.35 × 103 CFU/m3 to 0.70 × 103 CFU/m3, 2.76 × 103 CFU/m3 to 2.88 × 103 CFU/m3, and 2.10 × 103 CFU/m3 to 2.54 × 103 CFU/m3 before, during, and after manure removal, respectively. The airborne bacteria concentrations were significantly increased by the manure removal process. According to LEfSe analysis results, g_Escherichia_Shigella, s_Escherichia_coli, o_Enterobacteriales, f_Enterobacteriaceae could be suggested as biomarkers during manure removal. The dominant bacterial phyla in the layer house monitored were Proteobacteria, Bacteroidetes, Firmicutes, and genera were Pseudomonas, Escherichia-Shigella, Comamonas. The predominant potential pathogenic bacteria in the layer house were Pseudomonas, Escherichia, Enterobacter, and Acinetobacter.
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19 May 2022
A Correction to this paper has been published: https://doi.org/10.1007/s11270-022-05621-w
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Funding
This work was supported in part by the National Natural Science Foundation of China (31672467), the National Science Foundation for Young Scientists of China (41605094), and the Ningbo Science and Technology Bureau Public Welfare Program (2019C10076). We also thank Ningbo Yuejun Layer Farm for their supplying as the field experimental site.
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X. Dai: field measurements, formal analysis, data processing, resources, writing—original draft preparation, coordination. X. Wang: investigation, field measurements, writing—reviewing and editing. A. Wang: field measurements, data curation. P. Bao: resources, methodology. L. Wang-Li: writing—reviewing and editing. H. Xiao: resources, writing—review and editing, supervision. K. Wang: conceptualization, supervision, project administration, funding acquisition.
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Dai, X., Wang, X., Wang, A. et al. Identification of Size-segregated Bioaerosol Community and Pathogenic Bacteria in a Tunnel-ventilated Layer House: Effect of Manure Removal. Water Air Soil Pollut 233, 85 (2022). https://doi.org/10.1007/s11270-022-05536-6
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DOI: https://doi.org/10.1007/s11270-022-05536-6